Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp

Detalhes bibliográficos
Autor(a) principal: Guimarães, Elisalva Teixeira
Data de Publicação: 2011
Outros Autores: Cruz, Gabriela Silva, Jesus, Alan Araújo de, Carvalho, Acácia Fernandes Lacerda de, Rogatto, Silvia Regina, Pereira, Lygia da Veiga, Santos, Ricardo Ribeiro dos, Soares, Milena Botelho Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5311
Resumo: texto completo: acesso restrito. p.1247 – 1255.
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spelling Guimarães, Elisalva TeixeiraCruz, Gabriela SilvaJesus, Alan Araújo deCarvalho, Acácia Fernandes Lacerda deRogatto, Silvia ReginaPereira, Lygia da VeigaSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraGuimarães, Elisalva TeixeiraCruz, Gabriela SilvaJesus, Alan Araújo deCarvalho, Acácia Fernandes Lacerda deRogatto, Silvia ReginaPereira, Lygia da VeigaSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2012-02-07T18:24:53Z20111879-1506http://www.repositorio.ufba.br/ri/handle/ri/5311v. 56texto completo: acesso restrito. p.1247 – 1255.Objective: Several studies have demonstrated that human dental pulp is a source of mes- enchymal stem cells. To better understand the biological properties of these cells we isolated and characterized stem cells from the dental pulp of EGFP transgenic mice. Methods: The pulp tissue was gently separated from the roots of teeth extracted from C57BL/ 6 mice, and cultured under appropriate conditions. Flow cytometry, RT-PCR, light micros- copy (staining for alkaline phosphatase) and immunofluorescence were used to investigate the expression of stem cell markers. The presence of chromosomal abnormalities was evaluated by G banding. Results: The mouse dental pulp stem cells (mDPSC) were highly proliferative, plastic- adherent, and exhibited a polymorphic morphology predominantly with stellate or fusiform shapes. The presence of cell clusters was observed in cultures of mDPSC. Some cells were positive for alkaline phosphatase. The karyotype was normal until the 5th passage. The Pou5f1/Oct-4 and ZFP42/Rex-1, but not Nanog transcripts were detected in mDPSC. Flow cytometry and fluorescence analyses revealed the presence of a heterogeneous population positive for embryonic and mesenchymal cell markers. Adipogenic, chondrogenic and osteogenic differentiation was achieved after two weeks of cell culture under chemically defined in vitro conditions. In addition, some elongated cells spontaneously acquired a contraction capacity. Conclusions: Our results reinforce that the dental pulp is an important source of adult stem cells and encourage studies on therapeutic potential of mDPSC in experimental disease models.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-07T18:24:53Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0003996911001622-main.pdf: 630366 bytes, checksum: f8594f5ab87d76d968413377ab5b2ecf (MD5)Made available in DSpace on 2012-02-07T18:24:53Z (GMT). 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dc.title.pt_BR.fl_str_mv Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
dc.title.alternative.pt_BR.fl_str_mv Archives of Oral Biology
title Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
spellingShingle Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
Guimarães, Elisalva Teixeira
Dental pulp
Stem cells
Mouse
Phenotype
Differentiation
title_short Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
title_full Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
title_fullStr Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
title_full_unstemmed Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
title_sort Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
author Guimarães, Elisalva Teixeira
author_facet Guimarães, Elisalva Teixeira
Cruz, Gabriela Silva
Jesus, Alan Araújo de
Carvalho, Acácia Fernandes Lacerda de
Rogatto, Silvia Regina
Pereira, Lygia da Veiga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author_role author
author2 Cruz, Gabriela Silva
Jesus, Alan Araújo de
Carvalho, Acácia Fernandes Lacerda de
Rogatto, Silvia Regina
Pereira, Lygia da Veiga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Guimarães, Elisalva Teixeira
Cruz, Gabriela Silva
Jesus, Alan Araújo de
Carvalho, Acácia Fernandes Lacerda de
Rogatto, Silvia Regina
Pereira, Lygia da Veiga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
Guimarães, Elisalva Teixeira
Cruz, Gabriela Silva
Jesus, Alan Araújo de
Carvalho, Acácia Fernandes Lacerda de
Rogatto, Silvia Regina
Pereira, Lygia da Veiga
Santos, Ricardo Ribeiro dos
Soares, Milena Botelho Pereira
dc.subject.por.fl_str_mv Dental pulp
Stem cells
Mouse
Phenotype
Differentiation
topic Dental pulp
Stem cells
Mouse
Phenotype
Differentiation
description texto completo: acesso restrito. p.1247 – 1255.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-02-07T18:24:53Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5311
dc.identifier.issn.none.fl_str_mv 1879-1506
dc.identifier.number.pt_BR.fl_str_mv v. 56
identifier_str_mv 1879-1506
v. 56
url http://www.repositorio.ufba.br/ri/handle/ri/5311
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv doi:10.1016/j.archoralbio.2011.05.008
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