Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5311 |
Resumo: | texto completo: acesso restrito. p.1247 – 1255. |
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Guimarães, Elisalva TeixeiraCruz, Gabriela SilvaJesus, Alan Araújo deCarvalho, Acácia Fernandes Lacerda deRogatto, Silvia ReginaPereira, Lygia da VeigaSantos, Ricardo Ribeiro dosSoares, Milena Botelho PereiraGuimarães, Elisalva TeixeiraCruz, Gabriela SilvaJesus, Alan Araújo deCarvalho, Acácia Fernandes Lacerda deRogatto, Silvia ReginaPereira, Lygia da VeigaSantos, Ricardo Ribeiro dosSoares, Milena Botelho Pereira2012-02-07T18:24:53Z20111879-1506http://www.repositorio.ufba.br/ri/handle/ri/5311v. 56texto completo: acesso restrito. p.1247 – 1255.Objective: Several studies have demonstrated that human dental pulp is a source of mes- enchymal stem cells. To better understand the biological properties of these cells we isolated and characterized stem cells from the dental pulp of EGFP transgenic mice. Methods: The pulp tissue was gently separated from the roots of teeth extracted from C57BL/ 6 mice, and cultured under appropriate conditions. Flow cytometry, RT-PCR, light micros- copy (staining for alkaline phosphatase) and immunofluorescence were used to investigate the expression of stem cell markers. The presence of chromosomal abnormalities was evaluated by G banding. Results: The mouse dental pulp stem cells (mDPSC) were highly proliferative, plastic- adherent, and exhibited a polymorphic morphology predominantly with stellate or fusiform shapes. The presence of cell clusters was observed in cultures of mDPSC. Some cells were positive for alkaline phosphatase. The karyotype was normal until the 5th passage. The Pou5f1/Oct-4 and ZFP42/Rex-1, but not Nanog transcripts were detected in mDPSC. Flow cytometry and fluorescence analyses revealed the presence of a heterogeneous population positive for embryonic and mesenchymal cell markers. Adipogenic, chondrogenic and osteogenic differentiation was achieved after two weeks of cell culture under chemically defined in vitro conditions. In addition, some elongated cells spontaneously acquired a contraction capacity. Conclusions: Our results reinforce that the dental pulp is an important source of adult stem cells and encourage studies on therapeutic potential of mDPSC in experimental disease models.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-07T18:24:53Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0003996911001622-main.pdf: 630366 bytes, checksum: f8594f5ab87d76d968413377ab5b2ecf (MD5)Made available in DSpace on 2012-02-07T18:24:53Z (GMT). No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0003996911001622-main.pdf: 630366 bytes, checksum: f8594f5ab87d76d968413377ab5b2ecf (MD5) Previous issue date: 2011doi:10.1016/j.archoralbio.2011.05.008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBADental pulpStem cellsMousePhenotypeDifferentiationMesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulpArchives of Oral Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__pdn.sciencedirect.com_....0-S0003996911001622-main.pdf__pdn.sciencedirect.com_....0-S0003996911001622-main.pdfapplication/pdf630366https://repositorio.ufba.br/bitstream/ri/5311/1/__pdn.sciencedirect.com_....0-S0003996911001622-main.pdff8594f5ab87d76d968413377ab5b2ecfMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5311/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__pdn.sciencedirect.com_....0-S0003996911001622-main.pdf.txt__pdn.sciencedirect.com_....0-S0003996911001622-main.pdf.txtExtracted texttext/plain36319https://repositorio.ufba.br/bitstream/ri/5311/3/__pdn.sciencedirect.com_....0-S0003996911001622-main.pdf.txt146b8e237aa1c4e5b685d140dbff17a0MD53ri/53112022-07-05 14:03:21.001oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:21Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
dc.title.alternative.pt_BR.fl_str_mv |
Archives of Oral Biology |
title |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
spellingShingle |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp Guimarães, Elisalva Teixeira Dental pulp Stem cells Mouse Phenotype Differentiation |
title_short |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
title_full |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
title_fullStr |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
title_full_unstemmed |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
title_sort |
Mesenchymal and embryonic characteristics of stem cells obtained from mouse dental pulp |
author |
Guimarães, Elisalva Teixeira |
author_facet |
Guimarães, Elisalva Teixeira Cruz, Gabriela Silva Jesus, Alan Araújo de Carvalho, Acácia Fernandes Lacerda de Rogatto, Silvia Regina Pereira, Lygia da Veiga Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
author_role |
author |
author2 |
Cruz, Gabriela Silva Jesus, Alan Araújo de Carvalho, Acácia Fernandes Lacerda de Rogatto, Silvia Regina Pereira, Lygia da Veiga Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Guimarães, Elisalva Teixeira Cruz, Gabriela Silva Jesus, Alan Araújo de Carvalho, Acácia Fernandes Lacerda de Rogatto, Silvia Regina Pereira, Lygia da Veiga Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira Guimarães, Elisalva Teixeira Cruz, Gabriela Silva Jesus, Alan Araújo de Carvalho, Acácia Fernandes Lacerda de Rogatto, Silvia Regina Pereira, Lygia da Veiga Santos, Ricardo Ribeiro dos Soares, Milena Botelho Pereira |
dc.subject.por.fl_str_mv |
Dental pulp Stem cells Mouse Phenotype Differentiation |
topic |
Dental pulp Stem cells Mouse Phenotype Differentiation |
description |
texto completo: acesso restrito. p.1247 – 1255. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2012-02-07T18:24:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/5311 |
dc.identifier.issn.none.fl_str_mv |
1879-1506 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 56 |
identifier_str_mv |
1879-1506 v. 56 |
url |
http://www.repositorio.ufba.br/ri/handle/ri/5311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.pt_BR.fl_str_mv |
doi:10.1016/j.archoralbio.2011.05.008 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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Universidade Federal da Bahia (UFBA) |
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Repositório Institucional da UFBA |
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