Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis

Detalhes bibliográficos
Autor(a) principal: He,Junna
Data de Publicação: 2021
Outros Autores: Zhang,Xiangjian, He,Weiliang, Xie,Yanzhao, Chen,Yanxia, Yang,Yang, Chen,Rong
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608
Resumo: It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia.
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spelling Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosisCerebral ischemiaZonisamideNeuroprotectionApoptosisNeuronal cellsIt is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia.Associação Brasileira de Divulgação Científica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608Brazilian Journal of Medical and Biological Research v.54 n.4 2021reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x202010498info:eu-repo/semantics/openAccessHe,JunnaZhang,XiangjianHe,WeiliangXie,YanzhaoChen,YanxiaYang,YangChen,Rongeng2021-02-24T00:00:00Zoai:scielo:S0100-879X2021000400608Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2021-02-24T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
spellingShingle Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
He,Junna
Cerebral ischemia
Zonisamide
Neuroprotection
Apoptosis
Neuronal cells
title_short Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_full Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_fullStr Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_full_unstemmed Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
title_sort Neuroprotective effects of zonisamide on cerebral ischemia injury via inhibition of neuronal apoptosis
author He,Junna
author_facet He,Junna
Zhang,Xiangjian
He,Weiliang
Xie,Yanzhao
Chen,Yanxia
Yang,Yang
Chen,Rong
author_role author
author2 Zhang,Xiangjian
He,Weiliang
Xie,Yanzhao
Chen,Yanxia
Yang,Yang
Chen,Rong
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv He,Junna
Zhang,Xiangjian
He,Weiliang
Xie,Yanzhao
Chen,Yanxia
Yang,Yang
Chen,Rong
dc.subject.por.fl_str_mv Cerebral ischemia
Zonisamide
Neuroprotection
Apoptosis
Neuronal cells
topic Cerebral ischemia
Zonisamide
Neuroprotection
Apoptosis
Neuronal cells
description It is known that neuronal apoptosis contributes to pathology of cerebral ischemia injury. Zonisamide (ZNS) has shown anti-apoptosis effects in recent studies. The present study investigated whether the anti-apoptotic effect can account for the neuroprotective action of ZNS on cerebral ischemia. Neuronal cells were maintained under oxygen-glucose deprivation conditions to simulate cerebral ischemia and treated with ZNS simultaneously. The apoptosis of the cells and expression of apoptosis-related proteins were investigated by flow cytometry and western blot analysis, respectively. A cerebral ischemia mouse model was created via middle cerebral artery occlusion, and the mice were treated with ZNS. Neurological deficit scores and infarct volumes of the cerebral ischemia mice were measured. The apoptosis status of the neuronal cells was evaluated by TUNEL staining. In vitro, the ZNS treatment inhibited both the apoptosis of the neuronal cells and apoptosis-related protein expression (caspase-3, caspase-8, and calpain-1) induced by the oxygen-glucose deprivation. The anti-apoptosis effect of ZNS could occur through the blocking of reactive oxygen species. Moreover, ZNS treatment significantly ameliorated neurological deficits and reduced infarct volumes in the cerebral ischemia mice model. In this study, ZNS exerted neuroprotective effects by inhibition of apoptosis in neuronal cells in cerebral ischemia. Therefore, ZNS might be a promising therapy for cerebral ischemia.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400608
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431x202010498
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.54 n.4 2021
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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