Use of fluorescent probes to follow membrane traffic in nerve terminals

Detalhes bibliográficos
Autor(a) principal: Guatimosim,C.
Data de Publicação: 1998
Outros Autores: Romano-Silva,M.A., Gomez,M.V., Prado,M.A.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018
Resumo: Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.
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spelling Use of fluorescent probes to follow membrane traffic in nerve terminalslaser scanning confocal microscopyFM1-43exocytosissynaptic vesiclesendocytosisOptical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.Associação Brasileira de Divulgação Científica1998-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018Brazilian Journal of Medical and Biological Research v.31 n.11 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998001100018info:eu-repo/semantics/openAccessGuatimosim,C.Romano-Silva,M.A.Gomez,M.V.Prado,M.A.M.eng1998-10-20T00:00:00Zoai:scielo:S0100-879X1998001100018Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Use of fluorescent probes to follow membrane traffic in nerve terminals
title Use of fluorescent probes to follow membrane traffic in nerve terminals
spellingShingle Use of fluorescent probes to follow membrane traffic in nerve terminals
Guatimosim,C.
laser scanning confocal microscopy
FM1-43
exocytosis
synaptic vesicles
endocytosis
title_short Use of fluorescent probes to follow membrane traffic in nerve terminals
title_full Use of fluorescent probes to follow membrane traffic in nerve terminals
title_fullStr Use of fluorescent probes to follow membrane traffic in nerve terminals
title_full_unstemmed Use of fluorescent probes to follow membrane traffic in nerve terminals
title_sort Use of fluorescent probes to follow membrane traffic in nerve terminals
author Guatimosim,C.
author_facet Guatimosim,C.
Romano-Silva,M.A.
Gomez,M.V.
Prado,M.A.M.
author_role author
author2 Romano-Silva,M.A.
Gomez,M.V.
Prado,M.A.M.
author2_role author
author
author
dc.contributor.author.fl_str_mv Guatimosim,C.
Romano-Silva,M.A.
Gomez,M.V.
Prado,M.A.M.
dc.subject.por.fl_str_mv laser scanning confocal microscopy
FM1-43
exocytosis
synaptic vesicles
endocytosis
topic laser scanning confocal microscopy
FM1-43
exocytosis
synaptic vesicles
endocytosis
description Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.
publishDate 1998
dc.date.none.fl_str_mv 1998-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X1998001100018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.31 n.11 1998
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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