Use of fluorescent probes to follow membrane traffic in nerve terminals
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018 |
Resumo: | Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals. |
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Brazilian Journal of Medical and Biological Research |
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Use of fluorescent probes to follow membrane traffic in nerve terminalslaser scanning confocal microscopyFM1-43exocytosissynaptic vesiclesendocytosisOptical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals.Associação Brasileira de Divulgação Científica1998-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018Brazilian Journal of Medical and Biological Research v.31 n.11 1998reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1998001100018info:eu-repo/semantics/openAccessGuatimosim,C.Romano-Silva,M.A.Gomez,M.V.Prado,M.A.M.eng1998-10-20T00:00:00Zoai:scielo:S0100-879X1998001100018Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1998-10-20T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
title |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
spellingShingle |
Use of fluorescent probes to follow membrane traffic in nerve terminals Guatimosim,C. laser scanning confocal microscopy FM1-43 exocytosis synaptic vesicles endocytosis |
title_short |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
title_full |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
title_fullStr |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
title_full_unstemmed |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
title_sort |
Use of fluorescent probes to follow membrane traffic in nerve terminals |
author |
Guatimosim,C. |
author_facet |
Guatimosim,C. Romano-Silva,M.A. Gomez,M.V. Prado,M.A.M. |
author_role |
author |
author2 |
Romano-Silva,M.A. Gomez,M.V. Prado,M.A.M. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Guatimosim,C. Romano-Silva,M.A. Gomez,M.V. Prado,M.A.M. |
dc.subject.por.fl_str_mv |
laser scanning confocal microscopy FM1-43 exocytosis synaptic vesicles endocytosis |
topic |
laser scanning confocal microscopy FM1-43 exocytosis synaptic vesicles endocytosis |
description |
Optical tracers in conjunction with fluorescence microscopy have become widely used to follow the movement of synaptic vesicles in nerve terminals. The present review discusses the use of these optical methods to understand the regulation of exocytosis and endocytosis of synaptic vesicles. The maintenance of neurotransmission depends on the constant recycling of synaptic vesicles and important insights have been gained by visualization of vesicles with the vital dye FM1-43. A number of questions related to the control of recycling of synaptic vesicles by prolonged stimulation and the role of calcium to control membrane internalization are now being addressed. It is expected that optical monitoring of presynaptic activity coupled to appropriate genetic models will contribute to the understanding of membrane traffic in synaptic terminals. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X1998001100018 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.31 n.11 1998 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302929621221376 |