Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes

Detalhes bibliográficos
Autor(a) principal: Machado,C.G.
Data de Publicação: 2003
Outros Autores: Calado,R.T., Garcia,A.B., Falcão,R.P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006
Resumo: The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging.
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spelling Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytesP-glycoproteinMultidrug resistanceLymphocytesRhodamine 123Flow cytometryThe multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging.Associação Brasileira de Divulgação Científica2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006Brazilian Journal of Medical and Biological Research v.36 n.12 2003reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2003001200006info:eu-repo/semantics/openAccessMachado,C.G.Calado,R.T.Garcia,A.B.Falcão,R.P.eng2003-11-17T00:00:00Zoai:scielo:S0100-879X2003001200006Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2003-11-17T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
title Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
spellingShingle Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
Machado,C.G.
P-glycoprotein
Multidrug resistance
Lymphocytes
Rhodamine 123
Flow cytometry
title_short Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
title_full Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
title_fullStr Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
title_full_unstemmed Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
title_sort Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
author Machado,C.G.
author_facet Machado,C.G.
Calado,R.T.
Garcia,A.B.
Falcão,R.P.
author_role author
author2 Calado,R.T.
Garcia,A.B.
Falcão,R.P.
author2_role author
author
author
dc.contributor.author.fl_str_mv Machado,C.G.
Calado,R.T.
Garcia,A.B.
Falcão,R.P.
dc.subject.por.fl_str_mv P-glycoprotein
Multidrug resistance
Lymphocytes
Rhodamine 123
Flow cytometry
topic P-glycoprotein
Multidrug resistance
Lymphocytes
Rhodamine 123
Flow cytometry
description The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging.
publishDate 2003
dc.date.none.fl_str_mv 2003-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2003001200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.36 n.12 2003
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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