Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006 |
Resumo: | The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging. |
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Brazilian Journal of Medical and Biological Research |
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Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytesP-glycoproteinMultidrug resistanceLymphocytesRhodamine 123Flow cytometryThe multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging.Associação Brasileira de Divulgação Científica2003-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006Brazilian Journal of Medical and Biological Research v.36 n.12 2003reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2003001200006info:eu-repo/semantics/openAccessMachado,C.G.Calado,R.T.Garcia,A.B.Falcão,R.P.eng2003-11-17T00:00:00Zoai:scielo:S0100-879X2003001200006Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2003-11-17T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
title |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
spellingShingle |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes Machado,C.G. P-glycoprotein Multidrug resistance Lymphocytes Rhodamine 123 Flow cytometry |
title_short |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
title_full |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
title_fullStr |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
title_full_unstemmed |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
title_sort |
Age-related changes of the multidrug resistance P-glycoprotein function in normal human peripheral blood T lymphocytes |
author |
Machado,C.G. |
author_facet |
Machado,C.G. Calado,R.T. Garcia,A.B. Falcão,R.P. |
author_role |
author |
author2 |
Calado,R.T. Garcia,A.B. Falcão,R.P. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Machado,C.G. Calado,R.T. Garcia,A.B. Falcão,R.P. |
dc.subject.por.fl_str_mv |
P-glycoprotein Multidrug resistance Lymphocytes Rhodamine 123 Flow cytometry |
topic |
P-glycoprotein Multidrug resistance Lymphocytes Rhodamine 123 Flow cytometry |
description |
The multidrug resistance P-glycoprotein is a transmembrane efflux pump expressed by lymphocytes and is involved in their cytolytic activity. In the present study, we investigated the age-related changes of P-glycoprotein function in normal peripheral blood lymphocytes. Blood samples from 90 normal volunteers (age range, 0 to 86 years) were analyzed. P-glycoprotein function was assessed by the flow cytometric rhodamine 123 assay. P-glycoprotein function was highest in cord blood and progressively declined with age in peripheral blood T CD4+ and CD8+ cells. In contrast, P-glycoprotein function did not vary with age in CD19+ B or CD16+CD56+ natural killer cells. These data suggest that the decline in P-glycoprotein function in T CD4+ and CD8+ lymphocytes as a function of age may contribute to the decrease in T cell cytolytic activity with aging. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2003001200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.36 n.12 2003 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302932584497152 |