Oxidative stress is involved in LLLT mechanism of action on skin healing in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600608 |
Resumo: | The skin injury healing process involves the main phases of homoeostasis, inflammation, proliferation, and remodeling. The present study aimed to analyze the effects of low-level laser therapy (LLLT) on hematological dynamics, oxidative stress markers, and its relation with tissue healing following skin injury. Wistar rats were divided into control, sham, skin injury, and skin injury LLLT. The biochemical and morphological analyses were performed in the inflammatory (1 and 3 days) and regenerative phases (7, 14, and 21 days) following injury. The skin injury was performed in the dorsal region, between the intrascapular lines, using a surgical punch. LLLT (Al-Ga-In-P, λ=660 nm, energy density of 20 J/cm2, 30 mW power, and a time of 40 s) was applied at the area immediately after injury and on every following day according to the experimental subgroups. LLLT maintained hematocrit and hemoglobin levels until the 3rd day of treatment. Surprisingly, LLLT increased total leukocytes levels compared to control until the 3rd day. The effects of LLLT on mitochondrial activity were demonstrated by the significant increase in MTT levels in both inflammatory and regenerative phases (from the 1st to the 7th day), but only when associated with skin injury. The results indicated that LLLT modulated the inflammatory response intensity and accelerated skin tissue healing by a mechanism that involved oxidative damage reduction mostly at early stages of skin healing (inflammatory phase). |
id |
ABDC-1_98b3ff056fb4326cfe083d83a1efa331 |
---|---|
oai_identifier_str |
oai:scielo:S0100-879X2021000600608 |
network_acronym_str |
ABDC-1 |
network_name_str |
Brazilian Journal of Medical and Biological Research |
repository_id_str |
|
spelling |
Oxidative stress is involved in LLLT mechanism of action on skin healing in ratsRehabilitationPhototherapyLLLTSkin tissueInflammationThe skin injury healing process involves the main phases of homoeostasis, inflammation, proliferation, and remodeling. The present study aimed to analyze the effects of low-level laser therapy (LLLT) on hematological dynamics, oxidative stress markers, and its relation with tissue healing following skin injury. Wistar rats were divided into control, sham, skin injury, and skin injury LLLT. The biochemical and morphological analyses were performed in the inflammatory (1 and 3 days) and regenerative phases (7, 14, and 21 days) following injury. The skin injury was performed in the dorsal region, between the intrascapular lines, using a surgical punch. LLLT (Al-Ga-In-P, λ=660 nm, energy density of 20 J/cm2, 30 mW power, and a time of 40 s) was applied at the area immediately after injury and on every following day according to the experimental subgroups. LLLT maintained hematocrit and hemoglobin levels until the 3rd day of treatment. Surprisingly, LLLT increased total leukocytes levels compared to control until the 3rd day. The effects of LLLT on mitochondrial activity were demonstrated by the significant increase in MTT levels in both inflammatory and regenerative phases (from the 1st to the 7th day), but only when associated with skin injury. The results indicated that LLLT modulated the inflammatory response intensity and accelerated skin tissue healing by a mechanism that involved oxidative damage reduction mostly at early stages of skin healing (inflammatory phase).Associação Brasileira de Divulgação Científica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600608Brazilian Journal of Medical and Biological Research v.54 n.6 2021reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x202010293info:eu-repo/semantics/openAccessHartmann,D.D.Martins,R.P.Silva,T.C. daStefanello,S.T.Courtes,A.A.Gonçalves,D.F.Furtado,A.B.V.Duarte,B.S.L.Signori,L.U.Soares,F.A.A.Puntel,G.O.eng2021-04-23T00:00:00Zoai:scielo:S0100-879X2021000600608Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2021-04-23T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
title |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
spellingShingle |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats Hartmann,D.D. Rehabilitation Phototherapy LLLT Skin tissue Inflammation |
title_short |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
title_full |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
title_fullStr |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
title_full_unstemmed |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
title_sort |
Oxidative stress is involved in LLLT mechanism of action on skin healing in rats |
author |
Hartmann,D.D. |
author_facet |
Hartmann,D.D. Martins,R.P. Silva,T.C. da Stefanello,S.T. Courtes,A.A. Gonçalves,D.F. Furtado,A.B.V. Duarte,B.S.L. Signori,L.U. Soares,F.A.A. Puntel,G.O. |
author_role |
author |
author2 |
Martins,R.P. Silva,T.C. da Stefanello,S.T. Courtes,A.A. Gonçalves,D.F. Furtado,A.B.V. Duarte,B.S.L. Signori,L.U. Soares,F.A.A. Puntel,G.O. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Hartmann,D.D. Martins,R.P. Silva,T.C. da Stefanello,S.T. Courtes,A.A. Gonçalves,D.F. Furtado,A.B.V. Duarte,B.S.L. Signori,L.U. Soares,F.A.A. Puntel,G.O. |
dc.subject.por.fl_str_mv |
Rehabilitation Phototherapy LLLT Skin tissue Inflammation |
topic |
Rehabilitation Phototherapy LLLT Skin tissue Inflammation |
description |
The skin injury healing process involves the main phases of homoeostasis, inflammation, proliferation, and remodeling. The present study aimed to analyze the effects of low-level laser therapy (LLLT) on hematological dynamics, oxidative stress markers, and its relation with tissue healing following skin injury. Wistar rats were divided into control, sham, skin injury, and skin injury LLLT. The biochemical and morphological analyses were performed in the inflammatory (1 and 3 days) and regenerative phases (7, 14, and 21 days) following injury. The skin injury was performed in the dorsal region, between the intrascapular lines, using a surgical punch. LLLT (Al-Ga-In-P, λ=660 nm, energy density of 20 J/cm2, 30 mW power, and a time of 40 s) was applied at the area immediately after injury and on every following day according to the experimental subgroups. LLLT maintained hematocrit and hemoglobin levels until the 3rd day of treatment. Surprisingly, LLLT increased total leukocytes levels compared to control until the 3rd day. The effects of LLLT on mitochondrial activity were demonstrated by the significant increase in MTT levels in both inflammatory and regenerative phases (from the 1st to the 7th day), but only when associated with skin injury. The results indicated that LLLT modulated the inflammatory response intensity and accelerated skin tissue healing by a mechanism that involved oxidative damage reduction mostly at early stages of skin healing (inflammatory phase). |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600608 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000600608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x202010293 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.54 n.6 2021 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302948454694912 |