Immunological properties of gene vaccines delivered by different routes

Detalhes bibliográficos
Autor(a) principal: Oliveira,S.C.
Data de Publicação: 1999
Outros Autores: Rosinha,G.M.S., de-Brito,C.F.A., Fonseca,C.T., Afonso,R.R., Costa,M.C.M.S., Goes,A.M., Rech,E.L., Azevedo,V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009
Resumo: Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection raised a predominantly Th1 response with mostly IgG2a anti-ßgal produced, while gene gun immunization induced a mixed Th1/Th2 profile with a balanced production of IgG2a and IgG1 subclasses. Distinct types of immune responses were generated by different methods of gene delivery. These findings have important implications for genetic vaccine design. Firstly, a combination between these two systems may create optimal conditions for the induction of a broad-based immune response. Alternatively, a particular gene vaccine delivery method might be used according to the immune response required for host protection. Here, we describe the characteristics of the immune response induced by gene vaccination and the properties of DNA involved in this process.
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spelling Immunological properties of gene vaccines delivered by different routesgene vaccinationnaked DNAgene guncytokinesimmune responseGene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection raised a predominantly Th1 response with mostly IgG2a anti-ßgal produced, while gene gun immunization induced a mixed Th1/Th2 profile with a balanced production of IgG2a and IgG1 subclasses. Distinct types of immune responses were generated by different methods of gene delivery. These findings have important implications for genetic vaccine design. Firstly, a combination between these two systems may create optimal conditions for the induction of a broad-based immune response. Alternatively, a particular gene vaccine delivery method might be used according to the immune response required for host protection. Here, we describe the characteristics of the immune response induced by gene vaccination and the properties of DNA involved in this process.Associação Brasileira de Divulgação Científica1999-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009Brazilian Journal of Medical and Biological Research v.32 n.2 1999reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X1999000200009info:eu-repo/semantics/openAccessOliveira,S.C.Rosinha,G.M.S.de-Brito,C.F.A.Fonseca,C.T.Afonso,R.R.Costa,M.C.M.S.Goes,A.M.Rech,E.L.Azevedo,V.eng1999-02-04T00:00:00Zoai:scielo:S0100-879X1999000200009Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:1999-02-04T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Immunological properties of gene vaccines delivered by different routes
title Immunological properties of gene vaccines delivered by different routes
spellingShingle Immunological properties of gene vaccines delivered by different routes
Oliveira,S.C.
gene vaccination
naked DNA
gene gun
cytokines
immune response
title_short Immunological properties of gene vaccines delivered by different routes
title_full Immunological properties of gene vaccines delivered by different routes
title_fullStr Immunological properties of gene vaccines delivered by different routes
title_full_unstemmed Immunological properties of gene vaccines delivered by different routes
title_sort Immunological properties of gene vaccines delivered by different routes
author Oliveira,S.C.
author_facet Oliveira,S.C.
Rosinha,G.M.S.
de-Brito,C.F.A.
Fonseca,C.T.
Afonso,R.R.
Costa,M.C.M.S.
Goes,A.M.
Rech,E.L.
Azevedo,V.
author_role author
author2 Rosinha,G.M.S.
de-Brito,C.F.A.
Fonseca,C.T.
Afonso,R.R.
Costa,M.C.M.S.
Goes,A.M.
Rech,E.L.
Azevedo,V.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira,S.C.
Rosinha,G.M.S.
de-Brito,C.F.A.
Fonseca,C.T.
Afonso,R.R.
Costa,M.C.M.S.
Goes,A.M.
Rech,E.L.
Azevedo,V.
dc.subject.por.fl_str_mv gene vaccination
naked DNA
gene gun
cytokines
immune response
topic gene vaccination
naked DNA
gene gun
cytokines
immune response
description Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection raised a predominantly Th1 response with mostly IgG2a anti-ßgal produced, while gene gun immunization induced a mixed Th1/Th2 profile with a balanced production of IgG2a and IgG1 subclasses. Distinct types of immune responses were generated by different methods of gene delivery. These findings have important implications for genetic vaccine design. Firstly, a combination between these two systems may create optimal conditions for the induction of a broad-based immune response. Alternatively, a particular gene vaccine delivery method might be used according to the immune response required for host protection. Here, we describe the characteristics of the immune response induced by gene vaccination and the properties of DNA involved in this process.
publishDate 1999
dc.date.none.fl_str_mv 1999-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X1999000200009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.32 n.2 1999
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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