Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002 |
Resumo: | We investigated the day-night differences in intestinal oxidative-injury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period - 1 h after light onset) or evening (activity span - 13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P < 0.05) and TB (P < 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric acid reactive substance (TBARS) levels. Melatonin treatment in the morning (P < 0.05) or evening (P < 0.05) decreased TBARS levels after TB irradiation. After AP irradiation, melatonin treatment only in the morning caused a significant decrease in TBARS levels (P < 0.05). Although we have confirmed the development of inflammation after radiotherapy by histological findings, neither AP nor TB irradiation caused any marked changes in myeloperoxidase activity in the morning or evening. Our results indicate that oxidative damage is more prominent in rats receiving TB and AP irradiation in the morning and melatonin appears to have beneficial effects on oxidative damage irrespective of the time of administration. Increased neutrophil accumulation indicates that melatonin administration exerts a protective effect on AP irradiation-induced tissue oxidative injury, especially in the morning. |
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Brazilian Journal of Medical and Biological Research |
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Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestineIrradiationMelatoninCircadian rhythmOxidative stressThiobarbituric acid reactive substancesMyeloperoxidaseWe investigated the day-night differences in intestinal oxidative-injury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period - 1 h after light onset) or evening (activity span - 13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P < 0.05) and TB (P < 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric acid reactive substance (TBARS) levels. Melatonin treatment in the morning (P < 0.05) or evening (P < 0.05) decreased TBARS levels after TB irradiation. After AP irradiation, melatonin treatment only in the morning caused a significant decrease in TBARS levels (P < 0.05). Although we have confirmed the development of inflammation after radiotherapy by histological findings, neither AP nor TB irradiation caused any marked changes in myeloperoxidase activity in the morning or evening. Our results indicate that oxidative damage is more prominent in rats receiving TB and AP irradiation in the morning and melatonin appears to have beneficial effects on oxidative damage irrespective of the time of administration. Increased neutrophil accumulation indicates that melatonin administration exerts a protective effect on AP irradiation-induced tissue oxidative injury, especially in the morning.Associação Brasileira de Divulgação Científica2007-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002Brazilian Journal of Medical and Biological Research v.40 n.10 2007reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2006005000156info:eu-repo/semantics/openAccessGuney,Y.Hicsonmez,A.Uluoglu,C.Guney,H.Z.Ozel Turkcu,U.Take,G.Yucel,B.Caglar,G.Bilgihan,A.Erdogan,D.Nalca Andrieu,M.Kurtman,C.Zengil,H.eng2007-11-05T00:00:00Zoai:scielo:S0100-879X2007001000002Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2007-11-05T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
title |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
spellingShingle |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine Guney,Y. Irradiation Melatonin Circadian rhythm Oxidative stress Thiobarbituric acid reactive substances Myeloperoxidase |
title_short |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
title_full |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
title_fullStr |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
title_full_unstemmed |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
title_sort |
Melatonin prevents inflammation and oxidative stress caused by abdominopelvic and total body irradiation of rat small intestine |
author |
Guney,Y. |
author_facet |
Guney,Y. Hicsonmez,A. Uluoglu,C. Guney,H.Z. Ozel Turkcu,U. Take,G. Yucel,B. Caglar,G. Bilgihan,A. Erdogan,D. Nalca Andrieu,M. Kurtman,C. Zengil,H. |
author_role |
author |
author2 |
Hicsonmez,A. Uluoglu,C. Guney,H.Z. Ozel Turkcu,U. Take,G. Yucel,B. Caglar,G. Bilgihan,A. Erdogan,D. Nalca Andrieu,M. Kurtman,C. Zengil,H. |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Guney,Y. Hicsonmez,A. Uluoglu,C. Guney,H.Z. Ozel Turkcu,U. Take,G. Yucel,B. Caglar,G. Bilgihan,A. Erdogan,D. Nalca Andrieu,M. Kurtman,C. Zengil,H. |
dc.subject.por.fl_str_mv |
Irradiation Melatonin Circadian rhythm Oxidative stress Thiobarbituric acid reactive substances Myeloperoxidase |
topic |
Irradiation Melatonin Circadian rhythm Oxidative stress Thiobarbituric acid reactive substances Myeloperoxidase |
description |
We investigated the day-night differences in intestinal oxidative-injury and the inflammatory response following total body (TB) or abdominopelvic (AP) irradiation, and the influence of melatonin administration on tissue injury induced by radiation. Rats (male Wistar, weighing 220-280 g) in the irradiated groups were exposed to a dose of 8 Gy to the TB or AP region in the morning (resting period - 1 h after light onset) or evening (activity span - 13 h after light onset). Vehicle or melatonin was administered immediately before, immediately after and 24 h after irradiation (10, 2.0 and 10 mg/kg, ip, respectively) to the irradiated rats. AP (P < 0.05) and TB (P < 0.05) irradiation applied in the morning caused a significant increase in thiobarbituric acid reactive substance (TBARS) levels. Melatonin treatment in the morning (P < 0.05) or evening (P < 0.05) decreased TBARS levels after TB irradiation. After AP irradiation, melatonin treatment only in the morning caused a significant decrease in TBARS levels (P < 0.05). Although we have confirmed the development of inflammation after radiotherapy by histological findings, neither AP nor TB irradiation caused any marked changes in myeloperoxidase activity in the morning or evening. Our results indicate that oxidative damage is more prominent in rats receiving TB and AP irradiation in the morning and melatonin appears to have beneficial effects on oxidative damage irrespective of the time of administration. Increased neutrophil accumulation indicates that melatonin administration exerts a protective effect on AP irradiation-induced tissue oxidative injury, especially in the morning. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001000002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2006005000156 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.40 n.10 2007 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
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1754302936034312192 |