Immune response to respiratory syncytial virus in young Brazilian children
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011 |
Resumo: | We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV. |
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Brazilian Journal of Medical and Biological Research |
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Immune response to respiratory syncytial virus in young Brazilian childrenRespiratory syncytial virusPeripheral blood mononuclear cellsYoung childrenSurface antigensCytokinesWe have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV.Associação Brasileira de Divulgação Científica2002-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011Brazilian Journal of Medical and Biological Research v.35 n.10 2002reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2002001000011info:eu-repo/semantics/openAccessQueiróz,D.A.O.Durigon,E.L.Botosso,V.F.Ejzemberg,B.Vieira,S.E.Mineo,J.R.Yamashita,C.Hein,N.Lopes,C.L.Cacharo,A.L.Stewien,K.E.eng2002-10-13T00:00:00Zoai:scielo:S0100-879X2002001000011Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2002-10-13T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Immune response to respiratory syncytial virus in young Brazilian children |
title |
Immune response to respiratory syncytial virus in young Brazilian children |
spellingShingle |
Immune response to respiratory syncytial virus in young Brazilian children Queiróz,D.A.O. Respiratory syncytial virus Peripheral blood mononuclear cells Young children Surface antigens Cytokines |
title_short |
Immune response to respiratory syncytial virus in young Brazilian children |
title_full |
Immune response to respiratory syncytial virus in young Brazilian children |
title_fullStr |
Immune response to respiratory syncytial virus in young Brazilian children |
title_full_unstemmed |
Immune response to respiratory syncytial virus in young Brazilian children |
title_sort |
Immune response to respiratory syncytial virus in young Brazilian children |
author |
Queiróz,D.A.O. |
author_facet |
Queiróz,D.A.O. Durigon,E.L. Botosso,V.F. Ejzemberg,B. Vieira,S.E. Mineo,J.R. Yamashita,C. Hein,N. Lopes,C.L. Cacharo,A.L. Stewien,K.E. |
author_role |
author |
author2 |
Durigon,E.L. Botosso,V.F. Ejzemberg,B. Vieira,S.E. Mineo,J.R. Yamashita,C. Hein,N. Lopes,C.L. Cacharo,A.L. Stewien,K.E. |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Queiróz,D.A.O. Durigon,E.L. Botosso,V.F. Ejzemberg,B. Vieira,S.E. Mineo,J.R. Yamashita,C. Hein,N. Lopes,C.L. Cacharo,A.L. Stewien,K.E. |
dc.subject.por.fl_str_mv |
Respiratory syncytial virus Peripheral blood mononuclear cells Young children Surface antigens Cytokines |
topic |
Respiratory syncytial virus Peripheral blood mononuclear cells Young children Surface antigens Cytokines |
description |
We have evaluated the cellular and humoral immune response to primary respiratory syncytial virus (RSV) infection in young infants. Serum specimens from 65 patients <=12 months of age (39 males and 26 females, 28 cases <3 months and 37 cases > or = 3 months; median 3 ± 3.9 months) were tested for anti-RSV IgG and IgG subclass antibodies by EIA. Flow cytometry was used to characterize cell surface markers expressed on peripheral blood mononuclear cells (PBMC) from 29 RSV-infected children. There was a low rate of seroconversion in children <3 months of age, whose acute-phase PBMC were mostly T lymphocytes (63.0 ± 9.0%). In contrast, a higher rate of seroconversion was observed in children >3 months of age, with predominance of B lymphocytes (71.0 ± 17.7%). Stimulation of PBMC with RSV (2 x 10(5) TCID50) for 48 h did not induce a detectable increase in intracellular cytokines and only a few showed a detectable increase in RSV-specific secreted cytokines. These data suggest that age is an important factor affecting the infants' ability to develop an immune response to RSV. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002001000011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-879X2002001000011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.35 n.10 2002 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302932019314688 |