Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats

Detalhes bibliográficos
Autor(a) principal: Ribeiro,L.P.
Data de Publicação: 2018
Outros Autores: Freitas-Lima,L.C., Naumann,G.B., Meyrelles,S.S., Lunz,W., Pires,S.F., Andrade,H.M., Carnielli,J.B.T., Figueiredo,S.G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609
Resumo: In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (α-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, α-crystallin B chain and HSPβ-2). In addition, the cytoskeletal proteins desmin and α-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals.
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spelling Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred ratsInborn aerobic capacityProteomics2-DEMSHeart proteinsIn the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (α-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, α-crystallin B chain and HSPβ-2). In addition, the cytoskeletal proteins desmin and α-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals.Associação Brasileira de Divulgação Científica2018-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609Brazilian Journal of Medical and Biological Research v.51 n.3 2018reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20177033info:eu-repo/semantics/openAccessRibeiro,L.P.Freitas-Lima,L.C.Naumann,G.B.Meyrelles,S.S.Lunz,W.Pires,S.F.Andrade,H.M.Carnielli,J.B.T.Figueiredo,S.G.eng2019-03-19T00:00:00Zoai:scielo:S0100-879X2018000300609Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2019-03-19T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
title Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
spellingShingle Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
Ribeiro,L.P.
Inborn aerobic capacity
Proteomics
2-DE
MS
Heart proteins
title_short Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
title_full Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
title_fullStr Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
title_full_unstemmed Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
title_sort Cardiac protein expression patterns are associated with distinct inborn exercise capacity in non-selectively bred rats
author Ribeiro,L.P.
author_facet Ribeiro,L.P.
Freitas-Lima,L.C.
Naumann,G.B.
Meyrelles,S.S.
Lunz,W.
Pires,S.F.
Andrade,H.M.
Carnielli,J.B.T.
Figueiredo,S.G.
author_role author
author2 Freitas-Lima,L.C.
Naumann,G.B.
Meyrelles,S.S.
Lunz,W.
Pires,S.F.
Andrade,H.M.
Carnielli,J.B.T.
Figueiredo,S.G.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ribeiro,L.P.
Freitas-Lima,L.C.
Naumann,G.B.
Meyrelles,S.S.
Lunz,W.
Pires,S.F.
Andrade,H.M.
Carnielli,J.B.T.
Figueiredo,S.G.
dc.subject.por.fl_str_mv Inborn aerobic capacity
Proteomics
2-DE
MS
Heart proteins
topic Inborn aerobic capacity
Proteomics
2-DE
MS
Heart proteins
description In the present study, we successfully demonstrated for the first time the existence of cardiac proteomic differences between non-selectively bred rats with distinct intrinsic exercise capacities. A proteomic approach based on two-dimensional gel electrophoresis coupled to mass spectrometry was used to study the left ventricle (LV) tissue proteome of rats with distinct intrinsic exercise capacity. Low running performance (LRP) and high running performance (HRP) rats were categorized by a treadmill exercise test, according to distance run to exhaustion. The running capacity of HRPs was 3.5-fold greater than LRPs. Protein profiling revealed 29 differences between HRP and LRP rats (15 proteins were identified). We detected alterations in components involved in metabolism, antioxidant and stress response, microfibrillar and cytoskeletal proteins. Contractile proteins were upregulated in the LVs of HRP rats (α-myosin heavy chain-6, myosin light chain-1 and creatine kinase), whereas the LVs of LRP rats exhibited upregulation in proteins associated with stress response (aldehyde dehydrogenase 2, α-crystallin B chain and HSPβ-2). In addition, the cytoskeletal proteins desmin and α-actin were upregulated in LRPs. Taken together, our results suggest that the increased contractile protein levels in HRP rats partly accounted for their improved exercise capacity, and that proteins considered risk factors to the development of cardiovascular disease were expressed in higher amounts in LRP animals.
publishDate 2018
dc.date.none.fl_str_mv 2018-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300609
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1414-431x20177033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.51 n.3 2018
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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