Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits

Detalhes bibliográficos
Autor(a) principal: Castro,A.C.S.
Data de Publicação: 2002
Outros Autores: Berndtson,W.E., Cardoso,F.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Medical and Biological Research
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400014
Resumo: Plasma and tissue testosterone concentrations were determined by radioimmunoassay in 12 eight-month-old sexually mature New Zealand White rabbits and evaluated for possible associations with spermatogenic efficiency as well as with volume density and number of Leydig cells. Testicular tissue was processed histologically and histometry was performed in order to quantify germ cells, Sertoli cells and Leydig cells. Spermatogenic efficiency, reported as the ratios among germ cells (spermatogonia, primary spermatocytes and round spermatids) and by the ratio of germ cells to Sertoli cells, was not associated with testosterone levels. However, Leydig cell parameters such as number of Leydig cells per gram of testis, total number of Leydig cells per testis and percent cell volume of Leydig cell nuclei were correlated significantly with testosterone levels. The statistically significant correlation (r = 0.82, P<0.05) observed between testosterone levels and the number of Leydig cells per gram of testis suggests that, in the rabbit, the latter parameter can serve as a criterion for monitoring testosterone levels in this species under normal conditions.
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spelling Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbitsRabbitTestosteroneLeydig cellSpermatogenesisHistometryPlasma and tissue testosterone concentrations were determined by radioimmunoassay in 12 eight-month-old sexually mature New Zealand White rabbits and evaluated for possible associations with spermatogenic efficiency as well as with volume density and number of Leydig cells. Testicular tissue was processed histologically and histometry was performed in order to quantify germ cells, Sertoli cells and Leydig cells. Spermatogenic efficiency, reported as the ratios among germ cells (spermatogonia, primary spermatocytes and round spermatids) and by the ratio of germ cells to Sertoli cells, was not associated with testosterone levels. However, Leydig cell parameters such as number of Leydig cells per gram of testis, total number of Leydig cells per testis and percent cell volume of Leydig cell nuclei were correlated significantly with testosterone levels. The statistically significant correlation (r = 0.82, P<0.05) observed between testosterone levels and the number of Leydig cells per gram of testis suggests that, in the rabbit, the latter parameter can serve as a criterion for monitoring testosterone levels in this species under normal conditions.Associação Brasileira de Divulgação Científica2002-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400014Brazilian Journal of Medical and Biological Research v.35 n.4 2002reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/S0100-879X2002000400014info:eu-repo/semantics/openAccessCastro,A.C.S.Berndtson,W.E.Cardoso,F.M.eng2002-04-09T00:00:00Zoai:scielo:S0100-879X2002000400014Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2002-04-09T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false
dc.title.none.fl_str_mv Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
title Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
spellingShingle Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
Castro,A.C.S.
Rabbit
Testosterone
Leydig cell
Spermatogenesis
Histometry
title_short Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
title_full Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
title_fullStr Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
title_full_unstemmed Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
title_sort Plasma and testicular testosterone levels, volume density and number of Leydig cells and spermatogenic efficiency of rabbits
author Castro,A.C.S.
author_facet Castro,A.C.S.
Berndtson,W.E.
Cardoso,F.M.
author_role author
author2 Berndtson,W.E.
Cardoso,F.M.
author2_role author
author
dc.contributor.author.fl_str_mv Castro,A.C.S.
Berndtson,W.E.
Cardoso,F.M.
dc.subject.por.fl_str_mv Rabbit
Testosterone
Leydig cell
Spermatogenesis
Histometry
topic Rabbit
Testosterone
Leydig cell
Spermatogenesis
Histometry
description Plasma and tissue testosterone concentrations were determined by radioimmunoassay in 12 eight-month-old sexually mature New Zealand White rabbits and evaluated for possible associations with spermatogenic efficiency as well as with volume density and number of Leydig cells. Testicular tissue was processed histologically and histometry was performed in order to quantify germ cells, Sertoli cells and Leydig cells. Spermatogenic efficiency, reported as the ratios among germ cells (spermatogonia, primary spermatocytes and round spermatids) and by the ratio of germ cells to Sertoli cells, was not associated with testosterone levels. However, Leydig cell parameters such as number of Leydig cells per gram of testis, total number of Leydig cells per testis and percent cell volume of Leydig cell nuclei were correlated significantly with testosterone levels. The statistically significant correlation (r = 0.82, P<0.05) observed between testosterone levels and the number of Leydig cells per gram of testis suggests that, in the rabbit, the latter parameter can serve as a criterion for monitoring testosterone levels in this species under normal conditions.
publishDate 2002
dc.date.none.fl_str_mv 2002-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2002000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-879X2002000400014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv Brazilian Journal of Medical and Biological Research v.35 n.4 2002
reponame:Brazilian Journal of Medical and Biological Research
instname:Associação Brasileira de Divulgação Científica (ABDC)
instacron:ABDC
instname_str Associação Brasileira de Divulgação Científica (ABDC)
instacron_str ABDC
institution ABDC
reponame_str Brazilian Journal of Medical and Biological Research
collection Brazilian Journal of Medical and Biological Research
repository.name.fl_str_mv Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)
repository.mail.fl_str_mv bjournal@terra.com.br||bjournal@terra.com.br
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