Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion

Detalhes bibliográficos
Autor(a) principal: Saraiva, Karina Lidianne Alcântara
Data de Publicação: 2009
Outros Autores: Soares e Silva, Amanda Karolina, Wanderley, Maria Inês, Araújo, Araken Almeida de, Souza, José Roberto Botelho de, Peixoto, Christina Alves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27464
Resumo: Este trabalho foi apoiado pela Fundação Oswaldo Cruz (FIOCRUZ) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
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spelling Saraiva, Karina Lidianne AlcântaraSoares e Silva, Amanda KarolinaWanderley, Maria InêsAraújo, Araken Almeida deSouza, José Roberto Botelho dePeixoto, Christina Alves2018-07-11T11:14:18Z2018-07-11T11:14:18Z2009SARAIVA, K. L. A. et al. Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion. International Journal of Experimental Pathology, v. 90, n. 4, p. 454-462, Aug. 2009.1365-2613https://www.arca.fiocruz.br/handle/icict/2746410.1111/j.1365-2613.2009.00660.xEste trabalho foi apoiado pela Fundação Oswaldo Cruz (FIOCRUZ) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).2020-01-01Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil / Centro de Tecnologias Estratégicas do Nordeste. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Fisiologia e Farmacologia. Recife, PE, Brasil.Universidade de Pernambuco. Faculdade de Ciências Médicas. Recife, PE, Brasil.Universidade Federal de Pernambuco. Departamento de Zoologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Ultraestrutura. Recife, PE, Brasil / Centro de Tecnologias Estratégicas do Nordeste. Recife, PE, Brasil.The phosphodiesterase type 5 (PDE5) inhibitor, Sildenafil, is a novel, oral treatment approach for pulmonary hypertension. As Leydig cells present PDE5, this study was conducted to investigate the effects of the chronic treatment with Sildenafil (25 mg/kg) on male Swiss Webster mice steroidogenesis. After a 4-week long experimental design, Leydig cells were analysed by morphological and immunocytochemical procedures. Serum testosterone was assayed by radioimmunoassay. Leydig cells presented noteworthy ultrastructural alterations, such as a vesicular smooth endoplasmic reticulum, large vacuoles scattered through the cytoplasm, enlarged mitochondria with discontinue cristaes and whorle membranes with vesicles at the periphery, which are typical characteristics of an activated steroid-secreting cell. Important immunocytochemical labelling for steroidogenic acute regulatory protein, cytochrome P450 side-chain cleavage enzyme and testosterone were detected in isolated Leydig cells. In addition, Sildenafil-treated mice showed significant increased levels of total testosterone. The results obtained in the present study are consistent with the hypothesis that the accumulation of cyclic guanosine monophosphate by PDE5 inhibition could be involved in the androgen biosynthesis stimulation. Important clinical implications of hormonal disorders should be taken into account for patients with pulmonary hypertension.engCélulas de LeydigP450sccSildenafilStAREsteroidogêneseTestosteronaLeydig cellsP450sccSildenafilStARSteroidogenesisTestosteroneCélulas de Leydig / metabolismoInibidores da Fosfodiesterase 5Piperazinas / farmacologiaSulfonas / farmacologiaTestosterona / biossínteseAgentes vasodilatadores / farmacologiaChronic treatment with sildenafil stimulates Leydig cell and testosterone secretioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALChronic treatment with sildenafil stimulates Leydig cell and.pdfChronic treatment with sildenafil stimulates Leydig cell and.pdfapplication/pdf421895https://www.arca.fiocruz.br/bitstream/icict/27464/2/Chronic%20treatment%20with%20sildenafil%20stimulates%20Leydig%20cell%20and.pdffcc6ddd7048ae64106f0e97538b70297MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27464/3/license.txt5a560609d32a3863062d77ff32785d58MD53TEXTChronic treatment with sildenafil stimulates Leydig cell and.pdf.txtChronic treatment with sildenafil stimulates Leydig cell and.pdf.txtExtracted texttext/plain40975https://www.arca.fiocruz.br/bitstream/icict/27464/4/Chronic%20treatment%20with%20sildenafil%20stimulates%20Leydig%20cell%20and.pdf.txt71e496cd89385aaa858f093c47c6849fMD54icict/274642021-03-24 16:32:27.073oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:27Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
title Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
spellingShingle Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
Saraiva, Karina Lidianne Alcântara
Células de Leydig
P450scc
Sildenafil
StAR
Esteroidogênese
Testosterona
Leydig cells
P450scc
Sildenafil
StAR
Steroidogenesis
Testosterone
Células de Leydig / metabolismo
Inibidores da Fosfodiesterase 5
Piperazinas / farmacologia
Sulfonas / farmacologia
Testosterona / biossíntese
Agentes vasodilatadores / farmacologia
title_short Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
title_full Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
title_fullStr Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
title_full_unstemmed Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
title_sort Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion
author Saraiva, Karina Lidianne Alcântara
author_facet Saraiva, Karina Lidianne Alcântara
Soares e Silva, Amanda Karolina
Wanderley, Maria Inês
Araújo, Araken Almeida de
Souza, José Roberto Botelho de
Peixoto, Christina Alves
author_role author
author2 Soares e Silva, Amanda Karolina
Wanderley, Maria Inês
Araújo, Araken Almeida de
Souza, José Roberto Botelho de
Peixoto, Christina Alves
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Saraiva, Karina Lidianne Alcântara
Soares e Silva, Amanda Karolina
Wanderley, Maria Inês
Araújo, Araken Almeida de
Souza, José Roberto Botelho de
Peixoto, Christina Alves
dc.subject.other.none.fl_str_mv Células de Leydig
P450scc
Sildenafil
StAR
Esteroidogênese
Testosterona
topic Células de Leydig
P450scc
Sildenafil
StAR
Esteroidogênese
Testosterona
Leydig cells
P450scc
Sildenafil
StAR
Steroidogenesis
Testosterone
Células de Leydig / metabolismo
Inibidores da Fosfodiesterase 5
Piperazinas / farmacologia
Sulfonas / farmacologia
Testosterona / biossíntese
Agentes vasodilatadores / farmacologia
dc.subject.en.none.fl_str_mv Leydig cells
P450scc
Sildenafil
StAR
Steroidogenesis
Testosterone
dc.subject.decs.none.fl_str_mv Células de Leydig / metabolismo
Inibidores da Fosfodiesterase 5
Piperazinas / farmacologia
Sulfonas / farmacologia
Testosterona / biossíntese
Agentes vasodilatadores / farmacologia
description Este trabalho foi apoiado pela Fundação Oswaldo Cruz (FIOCRUZ) e Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-07-11T11:14:18Z
dc.date.available.fl_str_mv 2018-07-11T11:14:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SARAIVA, K. L. A. et al. Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion. International Journal of Experimental Pathology, v. 90, n. 4, p. 454-462, Aug. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27464
dc.identifier.issn.pt_BR.fl_str_mv 1365-2613
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-2613.2009.00660.x
identifier_str_mv SARAIVA, K. L. A. et al. Chronic treatment with sildenafil stimulates Leydig cell and testosterone secretion. International Journal of Experimental Pathology, v. 90, n. 4, p. 454-462, Aug. 2009.
1365-2613
10.1111/j.1365-2613.2009.00660.x
url https://www.arca.fiocruz.br/handle/icict/27464
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27464/2/Chronic%20treatment%20with%20sildenafil%20stimulates%20Leydig%20cell%20and.pdf
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