Differential proteomic analysis of children infected with respiratory syncytial virus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Medical and Biological Research |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609 |
Resumo: | Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection. |
id |
ABDC-1_fa63d1e2868282de96449a4848c45897 |
---|---|
oai_identifier_str |
oai:scielo:S0100-879X2021000400609 |
network_acronym_str |
ABDC-1 |
network_name_str |
Brazilian Journal of Medical and Biological Research |
repository_id_str |
|
spelling |
Differential proteomic analysis of children infected with respiratory syncytial virusChildrenRespiratory syncytial virusMouse modelDifferentially expressed proteinBiomarkersRespiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection.Associação Brasileira de Divulgação Científica2021-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609Brazilian Journal of Medical and Biological Research v.54 n.4 2021reponame:Brazilian Journal of Medical and Biological Researchinstname:Associação Brasileira de Divulgação Científica (ABDC)instacron:ABDC10.1590/1414-431x20209850info:eu-repo/semantics/openAccessYin,Gen-QuanZeng,Hui-XuanLi,Zi-LongChen,ChenZhong,Jia-YongXiao,Mi-SiZeng,QiangJiang,Wen-HuiWu,Pei-QiongZeng,Jie-MinHu,Xiao-YinChen,Huan-HuiRuo-Hu,Zhao,Hai-JinGao,LinLiu,CongCai,Shao-Xieng2021-02-24T00:00:00Zoai:scielo:S0100-879X2021000400609Revistahttps://www.bjournal.org/https://old.scielo.br/oai/scielo-oai.phpbjournal@terra.com.br||bjournal@terra.com.br1414-431X0100-879Xopendoar:2021-02-24T00:00Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC)false |
dc.title.none.fl_str_mv |
Differential proteomic analysis of children infected with respiratory syncytial virus |
title |
Differential proteomic analysis of children infected with respiratory syncytial virus |
spellingShingle |
Differential proteomic analysis of children infected with respiratory syncytial virus Yin,Gen-Quan Children Respiratory syncytial virus Mouse model Differentially expressed protein Biomarkers |
title_short |
Differential proteomic analysis of children infected with respiratory syncytial virus |
title_full |
Differential proteomic analysis of children infected with respiratory syncytial virus |
title_fullStr |
Differential proteomic analysis of children infected with respiratory syncytial virus |
title_full_unstemmed |
Differential proteomic analysis of children infected with respiratory syncytial virus |
title_sort |
Differential proteomic analysis of children infected with respiratory syncytial virus |
author |
Yin,Gen-Quan |
author_facet |
Yin,Gen-Quan Zeng,Hui-Xuan Li,Zi-Long Chen,Chen Zhong,Jia-Yong Xiao,Mi-Si Zeng,Qiang Jiang,Wen-Hui Wu,Pei-Qiong Zeng,Jie-Min Hu,Xiao-Yin Chen,Huan-Hui Ruo-Hu, Zhao,Hai-Jin Gao,Lin Liu,Cong Cai,Shao-Xi |
author_role |
author |
author2 |
Zeng,Hui-Xuan Li,Zi-Long Chen,Chen Zhong,Jia-Yong Xiao,Mi-Si Zeng,Qiang Jiang,Wen-Hui Wu,Pei-Qiong Zeng,Jie-Min Hu,Xiao-Yin Chen,Huan-Hui Ruo-Hu, Zhao,Hai-Jin Gao,Lin Liu,Cong Cai,Shao-Xi |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Yin,Gen-Quan Zeng,Hui-Xuan Li,Zi-Long Chen,Chen Zhong,Jia-Yong Xiao,Mi-Si Zeng,Qiang Jiang,Wen-Hui Wu,Pei-Qiong Zeng,Jie-Min Hu,Xiao-Yin Chen,Huan-Hui Ruo-Hu, Zhao,Hai-Jin Gao,Lin Liu,Cong Cai,Shao-Xi |
dc.subject.por.fl_str_mv |
Children Respiratory syncytial virus Mouse model Differentially expressed protein Biomarkers |
topic |
Children Respiratory syncytial virus Mouse model Differentially expressed protein Biomarkers |
description |
Respiratory syncytial virus (RSV) infection is the main cause of lower respiratory tract infection in children. However, there is no effective treatment for RSV infection. Here, we aimed to identify potential biomarkers to aid in the treatment of RSV infection. Children in the acute and convalescence phases of RSV infection were recruited and proteomic analysis was performed to identify differentially expressed proteins (DEPs). Subsequently, promising candidate proteins were determined by functional enrichment and protein-protein interaction network analysis, and underwent further validation by western blot both in clinical and mouse model samples. Among the 79 DEPs identified in RSV patient samples, 4 proteins (BPGM, TPI1, PRDX2, and CFL1) were confirmed to be significantly upregulated during RSV infection. Functional analysis showed that BPGM and TPI1 were mainly involved in glycolysis, indicating an association between RSV infection and the glycolysis metabolic pathway. Our findings provide insights into the proteomic profile during RSV infection and indicated that BPGM, TPI1, PRDX2, and CFL1 may be potential therapeutic biomarkers or targets for the treatment of RSV infection. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2021000400609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/1414-431x20209850 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
publisher.none.fl_str_mv |
Associação Brasileira de Divulgação Científica |
dc.source.none.fl_str_mv |
Brazilian Journal of Medical and Biological Research v.54 n.4 2021 reponame:Brazilian Journal of Medical and Biological Research instname:Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
instname_str |
Associação Brasileira de Divulgação Científica (ABDC) |
instacron_str |
ABDC |
institution |
ABDC |
reponame_str |
Brazilian Journal of Medical and Biological Research |
collection |
Brazilian Journal of Medical and Biological Research |
repository.name.fl_str_mv |
Brazilian Journal of Medical and Biological Research - Associação Brasileira de Divulgação Científica (ABDC) |
repository.mail.fl_str_mv |
bjournal@terra.com.br||bjournal@terra.com.br |
_version_ |
1754302948403314688 |