Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000200008 |
Resumo: | To change the recalcitrant nature of the lignocellulosic material for maximum hydrolysis yield, a comprehensive study was done by using sulphuric acid as an exclusive catalyst for the pretreatment process. The enzymatic digestibility of the biomass [Water Hyacinth: Eichhornia crassipes] after pretreatment was determined by measuring the hydrolysis yield of the pretreated material obtained from twenty four different pretreatment conditions. These included different concentrations of sulphuric acid (0.0, 1.0, 2.0 and 3.0%), at two different temperatures (108 and 121 ºC) for different residence times (1.0, 2.0 and 3.0h).The highest reducing sugar yield (36.65 g/L) from enzymatic hydrolysis was obtained when plant material was pretreated at 121 ºC for 1.0 h residence time using 3.0% (v/v) sulphuric acid and at 1:10 (w/v) solid to liquid ratio. The total reducing sugars obtained from the two-stage process (pretreatment + enzymatic hydrolysis) was 69.6g/L. The resulting sugars were fermented into ethanol by using Saccharomyces cerevisiae. The ethanol yield from the enzymatic hydrolyzate was 95.2% of the theoretical yield (0.51g/g glucose), as determined by GS-MS, and nearly 100% since no reducing sugars were detected in the fermenting media by TLC and DNS analysis. |
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Brazilian Journal of Chemical Engineering |
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Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol productionEichhornia crasspiesLignocellulosicWater hyacinthSaccharomyces cerevisiaeEthanolFermentationTo change the recalcitrant nature of the lignocellulosic material for maximum hydrolysis yield, a comprehensive study was done by using sulphuric acid as an exclusive catalyst for the pretreatment process. The enzymatic digestibility of the biomass [Water Hyacinth: Eichhornia crassipes] after pretreatment was determined by measuring the hydrolysis yield of the pretreated material obtained from twenty four different pretreatment conditions. These included different concentrations of sulphuric acid (0.0, 1.0, 2.0 and 3.0%), at two different temperatures (108 and 121 ºC) for different residence times (1.0, 2.0 and 3.0h).The highest reducing sugar yield (36.65 g/L) from enzymatic hydrolysis was obtained when plant material was pretreated at 121 ºC for 1.0 h residence time using 3.0% (v/v) sulphuric acid and at 1:10 (w/v) solid to liquid ratio. The total reducing sugars obtained from the two-stage process (pretreatment + enzymatic hydrolysis) was 69.6g/L. The resulting sugars were fermented into ethanol by using Saccharomyces cerevisiae. The ethanol yield from the enzymatic hydrolyzate was 95.2% of the theoretical yield (0.51g/g glucose), as determined by GS-MS, and nearly 100% since no reducing sugars were detected in the fermenting media by TLC and DNS analysis.Brazilian Society of Chemical Engineering2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000200008Brazilian Journal of Chemical Engineering v.31 n.2 2014reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20140312s00002415info:eu-repo/semantics/openAccessIdrees,M.Adnan,A.Bokhari,S. A.Qureshi,F. A.eng2014-07-07T00:00:00Zoai:scielo:S0104-66322014000200008Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2014-07-07T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
title |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
spellingShingle |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production Idrees,M. Eichhornia crasspies Lignocellulosic Water hyacinth Saccharomyces cerevisiae Ethanol Fermentation |
title_short |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
title_full |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
title_fullStr |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
title_full_unstemmed |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
title_sort |
Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production |
author |
Idrees,M. |
author_facet |
Idrees,M. Adnan,A. Bokhari,S. A. Qureshi,F. A. |
author_role |
author |
author2 |
Adnan,A. Bokhari,S. A. Qureshi,F. A. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Idrees,M. Adnan,A. Bokhari,S. A. Qureshi,F. A. |
dc.subject.por.fl_str_mv |
Eichhornia crasspies Lignocellulosic Water hyacinth Saccharomyces cerevisiae Ethanol Fermentation |
topic |
Eichhornia crasspies Lignocellulosic Water hyacinth Saccharomyces cerevisiae Ethanol Fermentation |
description |
To change the recalcitrant nature of the lignocellulosic material for maximum hydrolysis yield, a comprehensive study was done by using sulphuric acid as an exclusive catalyst for the pretreatment process. The enzymatic digestibility of the biomass [Water Hyacinth: Eichhornia crassipes] after pretreatment was determined by measuring the hydrolysis yield of the pretreated material obtained from twenty four different pretreatment conditions. These included different concentrations of sulphuric acid (0.0, 1.0, 2.0 and 3.0%), at two different temperatures (108 and 121 ºC) for different residence times (1.0, 2.0 and 3.0h).The highest reducing sugar yield (36.65 g/L) from enzymatic hydrolysis was obtained when plant material was pretreated at 121 ºC for 1.0 h residence time using 3.0% (v/v) sulphuric acid and at 1:10 (w/v) solid to liquid ratio. The total reducing sugars obtained from the two-stage process (pretreatment + enzymatic hydrolysis) was 69.6g/L. The resulting sugars were fermented into ethanol by using Saccharomyces cerevisiae. The ethanol yield from the enzymatic hydrolyzate was 95.2% of the theoretical yield (0.51g/g glucose), as determined by GS-MS, and nearly 100% since no reducing sugars were detected in the fermenting media by TLC and DNS analysis. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000200008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322014000200008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20140312s00002415 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.31 n.2 2014 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213174291202048 |