Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08

Detalhes bibliográficos
Autor(a) principal: Macedo,E. P.
Data de Publicação: 2013
Outros Autores: Cerqueira,C. L. O., Souza,D. A. J., Bispo,A. S. R., Coelho,R. R. R., Nascimento,R. P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005
Resumo: Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse.
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spelling Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08Streptomyces sp. SLBA-08Cellulose-degrading enzymeSisal bagasseSugarcane strawSugarcane bagasseSeveral types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse.Brazilian Society of Chemical Engineering2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005Brazilian Journal of Chemical Engineering v.30 n.4 2013reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322013000400005info:eu-repo/semantics/openAccessMacedo,E. P.Cerqueira,C. L. O.Souza,D. A. J.Bispo,A. S. R.Coelho,R. R. R.Nascimento,R. P.eng2014-01-10T00:00:00Zoai:scielo:S0104-66322013000400005Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2014-01-10T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
title Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
spellingShingle Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
Macedo,E. P.
Streptomyces sp. SLBA-08
Cellulose-degrading enzyme
Sisal bagasse
Sugarcane straw
Sugarcane bagasse
title_short Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
title_full Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
title_fullStr Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
title_full_unstemmed Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
title_sort Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
author Macedo,E. P.
author_facet Macedo,E. P.
Cerqueira,C. L. O.
Souza,D. A. J.
Bispo,A. S. R.
Coelho,R. R. R.
Nascimento,R. P.
author_role author
author2 Cerqueira,C. L. O.
Souza,D. A. J.
Bispo,A. S. R.
Coelho,R. R. R.
Nascimento,R. P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Macedo,E. P.
Cerqueira,C. L. O.
Souza,D. A. J.
Bispo,A. S. R.
Coelho,R. R. R.
Nascimento,R. P.
dc.subject.por.fl_str_mv Streptomyces sp. SLBA-08
Cellulose-degrading enzyme
Sisal bagasse
Sugarcane straw
Sugarcane bagasse
topic Streptomyces sp. SLBA-08
Cellulose-degrading enzyme
Sisal bagasse
Sugarcane straw
Sugarcane bagasse
description Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322013000400005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.30 n.4 2013
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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