Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005 |
Resumo: | Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse. |
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Brazilian Journal of Chemical Engineering |
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Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08Streptomyces sp. SLBA-08Cellulose-degrading enzymeSisal bagasseSugarcane strawSugarcane bagasseSeveral types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse.Brazilian Society of Chemical Engineering2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005Brazilian Journal of Chemical Engineering v.30 n.4 2013reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322013000400005info:eu-repo/semantics/openAccessMacedo,E. P.Cerqueira,C. L. O.Souza,D. A. J.Bispo,A. S. R.Coelho,R. R. R.Nascimento,R. P.eng2014-01-10T00:00:00Zoai:scielo:S0104-66322013000400005Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2014-01-10T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
title |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
spellingShingle |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 Macedo,E. P. Streptomyces sp. SLBA-08 Cellulose-degrading enzyme Sisal bagasse Sugarcane straw Sugarcane bagasse |
title_short |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
title_full |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
title_fullStr |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
title_full_unstemmed |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
title_sort |
Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08 |
author |
Macedo,E. P. |
author_facet |
Macedo,E. P. Cerqueira,C. L. O. Souza,D. A. J. Bispo,A. S. R. Coelho,R. R. R. Nascimento,R. P. |
author_role |
author |
author2 |
Cerqueira,C. L. O. Souza,D. A. J. Bispo,A. S. R. Coelho,R. R. R. Nascimento,R. P. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Macedo,E. P. Cerqueira,C. L. O. Souza,D. A. J. Bispo,A. S. R. Coelho,R. R. R. Nascimento,R. P. |
dc.subject.por.fl_str_mv |
Streptomyces sp. SLBA-08 Cellulose-degrading enzyme Sisal bagasse Sugarcane straw Sugarcane bagasse |
topic |
Streptomyces sp. SLBA-08 Cellulose-degrading enzyme Sisal bagasse Sugarcane straw Sugarcane bagasse |
description |
Several types of lignocellulosic raw materials including wheat straw, sisal bagasse and sugarcane bagasse have different contents of cellulosic components. In our studies, aimed at isolating cellulose-degrading actinobacteria from Brazilian soils, a promising strain was selected and identified as Streptomyces sp. SLBA-08. This strain was tested for cellulase production under submerged fermentation in a mineral medium using different carbon sources (sisal bagasse, sugarcane bagasse and straw), as well as ammonium sulphate in different concentrations as nitrogen source. The results showed that medium containing 2.4% (w/v) sisal bagasse and 0.3% (w/v) ammonium sulphate resulted in the highest production of carboxymethylcellulase (1.11 U mL-1), after 48 hours. The pH and temperature profile showed optimal activity at pH 6.0 and 50 °C. As for thermostability, carboxymethylcellulases were tolerant at 50 °C, retaining 70% of the maximal activity even after 2h of incubation. The results obtained indicate that Streptomyces sp. SLBA-08 was capable of producing CMCase using lignocellulosic residues, especially sisal bagasse. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322013000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322013000400005 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.30 n.4 2013 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213174214656000 |