BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100075 |
Resumo: | Abstract In the present study, a pure culture of bacterium (Pseudomonas sp. Strain NBM11) was isolated from the soil sample from a site contaminated with medical wastes and wastewater. The isolated strain can degrade up to 1000 mg/L of phenol completely. It was observed that temperature, pH and initial concentration of phenol play key roles in determining the rate of phenol degradation. The isolated strain exhibited the maximal degradation of the substrate within a range of pH 6.8 to 7.2 and an incubation temperature between 30 ºC and 32 ºC. It was found that by increasing the concentration of phenol, the lag phase gets extended due to the inhibitory nature of phenol. The kinetic parameters such as µmax (maximum specific growth rate), Ks (half-saturation coefficient) and Ki (substrate inhibition constant) were estimated as 0.184 1/h, 7.79 mg/L and 319.24 mg/L, respectively, by fitting the growth kinetics data to the Haldane model of substrate inhibition. The bacterial strain was immobilized in alginate beads and its phenol degradation efficiency was observed to increase many fold. The immobilized cells were found to be used efficiently for seven cycles consecutively without any decrease in their efficiency. |
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Brazilian Journal of Chemical Engineering |
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BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11Pseudomonas sp.ImmobilizationCalcium alginateAcinetobacter calcoaceticusPhenol BiodegradationAbstract In the present study, a pure culture of bacterium (Pseudomonas sp. Strain NBM11) was isolated from the soil sample from a site contaminated with medical wastes and wastewater. The isolated strain can degrade up to 1000 mg/L of phenol completely. It was observed that temperature, pH and initial concentration of phenol play key roles in determining the rate of phenol degradation. The isolated strain exhibited the maximal degradation of the substrate within a range of pH 6.8 to 7.2 and an incubation temperature between 30 ºC and 32 ºC. It was found that by increasing the concentration of phenol, the lag phase gets extended due to the inhibitory nature of phenol. The kinetic parameters such as µmax (maximum specific growth rate), Ks (half-saturation coefficient) and Ki (substrate inhibition constant) were estimated as 0.184 1/h, 7.79 mg/L and 319.24 mg/L, respectively, by fitting the growth kinetics data to the Haldane model of substrate inhibition. The bacterial strain was immobilized in alginate beads and its phenol degradation efficiency was observed to increase many fold. The immobilized cells were found to be used efficiently for seven cycles consecutively without any decrease in their efficiency.Brazilian Society of Chemical Engineering2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100075Brazilian Journal of Chemical Engineering v.34 n.1 2017reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20170341s20150388info:eu-repo/semantics/openAccessMohanty,Satya SundarJena,Hara Mohaneng2017-06-19T00:00:00Zoai:scielo:S0104-66322017000100075Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2017-06-19T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
title |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
spellingShingle |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 Mohanty,Satya Sundar Pseudomonas sp. Immobilization Calcium alginate Acinetobacter calcoaceticus Phenol Biodegradation |
title_short |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
title_full |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
title_fullStr |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
title_full_unstemmed |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
title_sort |
BIODEGRADATION OF PHENOL BY FREE AND IMMOBILIZED CELLS OF A NOVEL Pseudomonas sp. NBM11 |
author |
Mohanty,Satya Sundar |
author_facet |
Mohanty,Satya Sundar Jena,Hara Mohan |
author_role |
author |
author2 |
Jena,Hara Mohan |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Mohanty,Satya Sundar Jena,Hara Mohan |
dc.subject.por.fl_str_mv |
Pseudomonas sp. Immobilization Calcium alginate Acinetobacter calcoaceticus Phenol Biodegradation |
topic |
Pseudomonas sp. Immobilization Calcium alginate Acinetobacter calcoaceticus Phenol Biodegradation |
description |
Abstract In the present study, a pure culture of bacterium (Pseudomonas sp. Strain NBM11) was isolated from the soil sample from a site contaminated with medical wastes and wastewater. The isolated strain can degrade up to 1000 mg/L of phenol completely. It was observed that temperature, pH and initial concentration of phenol play key roles in determining the rate of phenol degradation. The isolated strain exhibited the maximal degradation of the substrate within a range of pH 6.8 to 7.2 and an incubation temperature between 30 ºC and 32 ºC. It was found that by increasing the concentration of phenol, the lag phase gets extended due to the inhibitory nature of phenol. The kinetic parameters such as µmax (maximum specific growth rate), Ks (half-saturation coefficient) and Ki (substrate inhibition constant) were estimated as 0.184 1/h, 7.79 mg/L and 319.24 mg/L, respectively, by fitting the growth kinetics data to the Haldane model of substrate inhibition. The bacterial strain was immobilized in alginate beads and its phenol degradation efficiency was observed to increase many fold. The immobilized cells were found to be used efficiently for seven cycles consecutively without any decrease in their efficiency. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100075 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100075 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20170341s20150388 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.34 n.1 2017 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213175441489920 |