VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100019 |
Resumo: | Abstract A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction. |
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Brazilian Journal of Chemical Engineering |
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VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMSColorimetric methodMicroplateOleaginous yeast and microalgaeLipidAbstract A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction.Brazilian Society of Chemical Engineering2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100019Brazilian Journal of Chemical Engineering v.34 n.1 2017reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/0104-6632.20170341s20140222info:eu-repo/semantics/openAccessAnschau,A.Caruso,C. S.Kuhn,R. C.Franco,T. T.eng2017-06-19T00:00:00Zoai:scielo:S0104-66322017000100019Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2017-06-19T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
title |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
spellingShingle |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS Anschau,A. Colorimetric method Microplate Oleaginous yeast and microalgae Lipid |
title_short |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
title_full |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
title_fullStr |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
title_full_unstemmed |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
title_sort |
VALIDATION OF THE SULFO-PHOSPHO-VANILLIN (SPV) METHOD FOR THE DETERMINATION OF LIPID CONTENT IN OLEAGINOUS MICROORGANISMS |
author |
Anschau,A. |
author_facet |
Anschau,A. Caruso,C. S. Kuhn,R. C. Franco,T. T. |
author_role |
author |
author2 |
Caruso,C. S. Kuhn,R. C. Franco,T. T. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Anschau,A. Caruso,C. S. Kuhn,R. C. Franco,T. T. |
dc.subject.por.fl_str_mv |
Colorimetric method Microplate Oleaginous yeast and microalgae Lipid |
topic |
Colorimetric method Microplate Oleaginous yeast and microalgae Lipid |
description |
Abstract A colorimetric sulfo-phospho-vanillin (SPV) method was used to validate a high throughput method for total lipids analysis in fresh and lyophilized oleaginous microorganisms. This method uses a small amount of biological material, does not require a lot of sample manipulation, can be automated, is reproducible and easy to implement. A 96-well microplate SPV assay allows one to determine quickly total lipids in lyophilized cells of oleaginous yeast and microalgae. The new assay method possesses many advantages compared to the others described in the literature: requires a small amount sample, less time (around 1 h) and less labor and does not require organic reagents like chloroform in the reaction. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100019 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322017000100019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-6632.20170341s20140222 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.34 n.1 2017 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
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1754213175433101312 |