Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus

Detalhes bibliográficos
Autor(a) principal: Baptista-Neto,A.
Data de Publicação: 2000
Outros Autores: Gouveia,E.R., Badino-Jr,A.C., Hokka,C.O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043
Resumo: The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.
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spelling Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerusclavulanic acidStreptomyces clavuligeruskinetic modelThe kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.Brazilian Society of Chemical Engineering2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043Brazilian Journal of Chemical Engineering v.17 n.4-7 2000reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322000000400043info:eu-repo/semantics/openAccessBaptista-Neto,A.Gouveia,E.R.Badino-Jr,A.C.Hokka,C.O.eng2001-03-16T00:00:00Zoai:scielo:S0104-66322000000400043Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2001-03-16T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
title Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
spellingShingle Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
Baptista-Neto,A.
clavulanic acid
Streptomyces clavuligerus
kinetic model
title_short Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
title_full Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
title_fullStr Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
title_full_unstemmed Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
title_sort Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
author Baptista-Neto,A.
author_facet Baptista-Neto,A.
Gouveia,E.R.
Badino-Jr,A.C.
Hokka,C.O.
author_role author
author2 Gouveia,E.R.
Badino-Jr,A.C.
Hokka,C.O.
author2_role author
author
author
dc.contributor.author.fl_str_mv Baptista-Neto,A.
Gouveia,E.R.
Badino-Jr,A.C.
Hokka,C.O.
dc.subject.por.fl_str_mv clavulanic acid
Streptomyces clavuligerus
kinetic model
topic clavulanic acid
Streptomyces clavuligerus
kinetic model
description The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.
publishDate 2000
dc.date.none.fl_str_mv 2000-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322000000400043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.17 n.4-7 2000
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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