Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2000 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043 |
Resumo: | The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide. |
id |
ABEQ-1_45754f6ea8ff44f82bfb5a451e6d237b |
---|---|
oai_identifier_str |
oai:scielo:S0104-66322000000400043 |
network_acronym_str |
ABEQ-1 |
network_name_str |
Brazilian Journal of Chemical Engineering |
repository_id_str |
|
spelling |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerusclavulanic acidStreptomyces clavuligeruskinetic modelThe kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide.Brazilian Society of Chemical Engineering2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043Brazilian Journal of Chemical Engineering v.17 n.4-7 2000reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322000000400043info:eu-repo/semantics/openAccessBaptista-Neto,A.Gouveia,E.R.Badino-Jr,A.C.Hokka,C.O.eng2001-03-16T00:00:00Zoai:scielo:S0104-66322000000400043Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2001-03-16T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
title |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
spellingShingle |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus Baptista-Neto,A. clavulanic acid Streptomyces clavuligerus kinetic model |
title_short |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
title_full |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
title_fullStr |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
title_full_unstemmed |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
title_sort |
Phenomenological model of the clavulanic acid production process utilizing Streptomyces clavuligerus |
author |
Baptista-Neto,A. |
author_facet |
Baptista-Neto,A. Gouveia,E.R. Badino-Jr,A.C. Hokka,C.O. |
author_role |
author |
author2 |
Gouveia,E.R. Badino-Jr,A.C. Hokka,C.O. |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Baptista-Neto,A. Gouveia,E.R. Badino-Jr,A.C. Hokka,C.O. |
dc.subject.por.fl_str_mv |
clavulanic acid Streptomyces clavuligerus kinetic model |
topic |
clavulanic acid Streptomyces clavuligerus kinetic model |
description |
The kinetics of clavulanic acid production process by Streptomyces clavuligerus NRRL 3585 was studied. Experiments were carried out in a 4 liters bioreactor, utilizing 2 complex media containing glycerol as the carbon and energy source, and peptone or Samprosoy 90NB (soybean protein) as nitrogen source. Temperature was kept at 28°C and the dissolved oxygen was controlled automatically at 40 % saturation value. Samples were withdrawn for determination of cell mass (only peptone medium), glycerol and product concentrations. Gas analyzers allowed on line determination of CO2 and O2 contents in the exit gas. With Samprosoy, cell mass was evaluated by determining glycerol consumption and considering the cell yield, Y X/S, as being the same for both cases. Oxygen uptake and CO2 production rates were strongly related to growth and substrate consumption, allowing determination of stoichiometric constants in relation to growth, substrate, oxygen, product and carbon dioxide. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322000000400043 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322000000400043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.17 n.4-7 2000 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213170781618176 |