Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Chemical Engineering |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001 |
Resumo: | The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cp max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L−1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis. |
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Brazilian Journal of Chemical Engineering |
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Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerusClavulanic acidStreptomyces clavuligerusGlycerolOrnithineFed-batchThe influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cp max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L−1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis.Brazilian Society of Chemical Engineering2010-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001Brazilian Journal of Chemical Engineering v.27 n.4 2010reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322010000400001info:eu-repo/semantics/openAccessTeodoro,J. C.Baptista-Neto,A.Araujo,M. L. G. C.Hokka,C. O.Badino,A. C.eng2011-01-04T00:00:00Zoai:scielo:S0104-66322010000400001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2011-01-04T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false |
dc.title.none.fl_str_mv |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
spellingShingle |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus Teodoro,J. C. Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
title_short |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_full |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_fullStr |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_full_unstemmed |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
title_sort |
Influence of glycerol and ornithine feeding on clavulanic acid production by Streptomyces clavuligerus |
author |
Teodoro,J. C. |
author_facet |
Teodoro,J. C. Baptista-Neto,A. Araujo,M. L. G. C. Hokka,C. O. Badino,A. C. |
author_role |
author |
author2 |
Baptista-Neto,A. Araujo,M. L. G. C. Hokka,C. O. Badino,A. C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Teodoro,J. C. Baptista-Neto,A. Araujo,M. L. G. C. Hokka,C. O. Badino,A. C. |
dc.subject.por.fl_str_mv |
Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
topic |
Clavulanic acid Streptomyces clavuligerus Glycerol Ornithine Fed-batch |
description |
The influence of glycerol and ornithine feeding on clavulanic acid (CA) production by Streptomyces clavuligerus was investigated. In batch experiments, CA maximum concentration (Cp max) ranged randomly from 430 to 560 mg.L-1, with a maximum increase of 10% in relation to the control run, without ornithine. However, the maximum volumetric productivity of CA (Pp max) of 13.7 mg.L-1.h-1 was obtained with 0.66 g.L-1 of ornithine, 44.2% higher than the Pp max in the control run. In fed-batch experiments, Cp max varied within the narrow range from 1.254 to 1.405 g.L-1, 2.5 times higher than that obtained in the control run. The presence of ornithine increased the Pp max, although it influenced only slightly the Cp max. Concerning glycerol, the highest CA production of 1.6 g.L-1 was obtained in the fed-batch with glycerol and ornithine (180 and 3.7 g.L−1) in a 10-L bioreactor, showing a positive effect of ornithine and glycerol, in the proper proportion (48.6:1), on CA biosynthesis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322010000400001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0104-66322010000400001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
publisher.none.fl_str_mv |
Brazilian Society of Chemical Engineering |
dc.source.none.fl_str_mv |
Brazilian Journal of Chemical Engineering v.27 n.4 2010 reponame:Brazilian Journal of Chemical Engineering instname:Associação Brasileira de Engenharia Química (ABEQ) instacron:ABEQ |
instname_str |
Associação Brasileira de Engenharia Química (ABEQ) |
instacron_str |
ABEQ |
institution |
ABEQ |
reponame_str |
Brazilian Journal of Chemical Engineering |
collection |
Brazilian Journal of Chemical Engineering |
repository.name.fl_str_mv |
Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ) |
repository.mail.fl_str_mv |
rgiudici@usp.br||rgiudici@usp.br |
_version_ |
1754213173140914176 |