Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey

Detalhes bibliográficos
Autor(a) principal: Rech,R.
Data de Publicação: 2006
Outros Autores: Ayub,M. A. Z.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322006000400001
Resumo: Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest beta-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.
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spelling Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese wheyRecombinant Saccharomyces cerevisiaebeta-galactosidaseCheese-wheyFed-batch cultivationSaccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest beta-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.Brazilian Society of Chemical Engineering2006-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322006000400001Brazilian Journal of Chemical Engineering v.23 n.4 2006reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322006000400001info:eu-repo/semantics/openAccessRech,R.Ayub,M. A. Z.eng2007-04-25T00:00:00Zoai:scielo:S0104-66322006000400001Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2007-04-25T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
spellingShingle Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
Rech,R.
Recombinant Saccharomyces cerevisiae
beta-galactosidase
Cheese-whey
Fed-batch cultivation
title_short Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_full Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_fullStr Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_full_unstemmed Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_sort Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
author Rech,R.
author_facet Rech,R.
Ayub,M. A. Z.
author_role author
author2 Ayub,M. A. Z.
author2_role author
dc.contributor.author.fl_str_mv Rech,R.
Ayub,M. A. Z.
dc.subject.por.fl_str_mv Recombinant Saccharomyces cerevisiae
beta-galactosidase
Cheese-whey
Fed-batch cultivation
topic Recombinant Saccharomyces cerevisiae
beta-galactosidase
Cheese-whey
Fed-batch cultivation
description Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify beta-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and beta-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest beta-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322006000400001
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322006000400001
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322006000400001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.23 n.4 2006
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
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