Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey

Detalhes bibliográficos
Autor(a) principal: Rech, Rosane
Data de Publicação: 2006
Outros Autores: Ayub, Marco Antônio Záchia
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/107573
Resumo: Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest β-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.
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spelling Rech, RosaneAyub, Marco Antônio Záchia2014-11-29T02:20:52Z20060104-6632http://hdl.handle.net/10183/107573000567377Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest β-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.application/pdfporBrazilian Journal of Chemical Engineering. São Paulo. vol. 23, n. 4 (oct./dec. 2006), p. 435-442Saccharomyces cerevisiae recombinanteSoro de leitebeta-GalactosidaseRecombinant Saccharomyces cerevisiaeβ-galactosidaseCheese-wheyFed-batch cultivationFed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese wheyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000567377.pdf000567377.pdfTexto completo (inglês)application/pdf230988http://www.lume.ufrgs.br/bitstream/10183/107573/1/000567377.pdf5ec5f59b3b239fbf03c47833ed2465f6MD51TEXT000567377.pdf.txt000567377.pdf.txtExtracted Texttext/plain26226http://www.lume.ufrgs.br/bitstream/10183/107573/2/000567377.pdf.txt348c0d58e96d1a956caf6404881ad7f0MD52THUMBNAIL000567377.pdf.jpg000567377.pdf.jpgGenerated Thumbnailimage/jpeg2015http://www.lume.ufrgs.br/bitstream/10183/107573/3/000567377.pdf.jpg9e0c358b23cfa6e92933bd84d79501dfMD5310183/1075732018-10-22 08:22:36.555oai:www.lume.ufrgs.br:10183/107573Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-22T11:22:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
spellingShingle Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
Rech, Rosane
Saccharomyces cerevisiae recombinante
Soro de leite
beta-Galactosidase
Recombinant Saccharomyces cerevisiae
β-galactosidase
Cheese-whey
Fed-batch cultivation
title_short Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_full Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_fullStr Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_full_unstemmed Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
title_sort Fed-batch bioreactor process with recombinant Saccharomyces cerevisiae growing on cheese whey
author Rech, Rosane
author_facet Rech, Rosane
Ayub, Marco Antônio Záchia
author_role author
author2 Ayub, Marco Antônio Záchia
author2_role author
dc.contributor.author.fl_str_mv Rech, Rosane
Ayub, Marco Antônio Záchia
dc.subject.por.fl_str_mv Saccharomyces cerevisiae recombinante
Soro de leite
beta-Galactosidase
topic Saccharomyces cerevisiae recombinante
Soro de leite
beta-Galactosidase
Recombinant Saccharomyces cerevisiae
β-galactosidase
Cheese-whey
Fed-batch cultivation
dc.subject.eng.fl_str_mv Recombinant Saccharomyces cerevisiae
β-galactosidase
Cheese-whey
Fed-batch cultivation
description Saccharomyces cerevisiae strain W303 was transformed with two yeast integrative plasmids containing Kluyveromyces lactis LAC4 and LAC12 genes that codify β-galactosidase and lactose permease respectively. The BLR030 recombinant strain was selected due to its growth and β-galactosidase production capacity. Different culture media based on deproteinized cheese whey (DCW) were tested and the best composition (containing DCW, supplemented with yeast extract 1 %, and peptone 3 % (w/v)) was chosen for bioreactor experiments. Batch, and fed-batch cultures with linear ascending feeding for 25 (FB25), 35 (FB35), and 50 (FB50) hours, were performed. FB35 and FB50 produced the highest β-galactosidase specific activities (around 1,800 U/g cells), and also the best productivities (180 U/L.h). Results show the potential use of fed-batch cultures of recombinant S. cerevisiae on industrial applications using supplemented whey as substrate.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2014-11-29T02:20:52Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/107573
dc.identifier.issn.pt_BR.fl_str_mv 0104-6632
dc.identifier.nrb.pt_BR.fl_str_mv 000567377
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url http://hdl.handle.net/10183/107573
dc.language.iso.fl_str_mv por
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Chemical Engineering. São Paulo. vol. 23, n. 4 (oct./dec. 2006), p. 435-442
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dc.format.none.fl_str_mv application/pdf
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