Performance of a pulsed-cap microcolumn for protein extraction

Detalhes bibliográficos
Autor(a) principal: Rabelo,A.P.B.
Data de Publicação: 2003
Outros Autores: Tambourgi,E.B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Chemical Engineering
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000400003
Resumo: This work presents the results obtained using a microcolumn agitated by pulsed caps, using aqueous two-phase systems formed polyethylene glycol and salts (monobasic and dibasic) potassium phosphate for protein extraction. Proteins used were extracted cytochrome b5 and the enzyme ascorbic oxidoreductase. It was observed that operation of this equipment was stable and high efficiency values were achieved.
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network_name_str Brazilian Journal of Chemical Engineering
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spelling Performance of a pulsed-cap microcolumn for protein extractionaqueous two-phase systemdownstream processingcontinuous extraction equipmentproteinpurificationsolvent extractionThis work presents the results obtained using a microcolumn agitated by pulsed caps, using aqueous two-phase systems formed polyethylene glycol and salts (monobasic and dibasic) potassium phosphate for protein extraction. Proteins used were extracted cytochrome b5 and the enzyme ascorbic oxidoreductase. It was observed that operation of this equipment was stable and high efficiency values were achieved.Brazilian Society of Chemical Engineering2003-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000400003Brazilian Journal of Chemical Engineering v.20 n.4 2003reponame:Brazilian Journal of Chemical Engineeringinstname:Associação Brasileira de Engenharia Química (ABEQ)instacron:ABEQ10.1590/S0104-66322003000400003info:eu-repo/semantics/openAccessRabelo,A.P.B.Tambourgi,E.B.eng2003-12-04T00:00:00Zoai:scielo:S0104-66322003000400003Revistahttps://www.scielo.br/j/bjce/https://old.scielo.br/oai/scielo-oai.phprgiudici@usp.br||rgiudici@usp.br1678-43830104-6632opendoar:2003-12-04T00:00Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)false
dc.title.none.fl_str_mv Performance of a pulsed-cap microcolumn for protein extraction
title Performance of a pulsed-cap microcolumn for protein extraction
spellingShingle Performance of a pulsed-cap microcolumn for protein extraction
Rabelo,A.P.B.
aqueous two-phase system
downstream processing
continuous extraction equipment
protein
purification
solvent extraction
title_short Performance of a pulsed-cap microcolumn for protein extraction
title_full Performance of a pulsed-cap microcolumn for protein extraction
title_fullStr Performance of a pulsed-cap microcolumn for protein extraction
title_full_unstemmed Performance of a pulsed-cap microcolumn for protein extraction
title_sort Performance of a pulsed-cap microcolumn for protein extraction
author Rabelo,A.P.B.
author_facet Rabelo,A.P.B.
Tambourgi,E.B.
author_role author
author2 Tambourgi,E.B.
author2_role author
dc.contributor.author.fl_str_mv Rabelo,A.P.B.
Tambourgi,E.B.
dc.subject.por.fl_str_mv aqueous two-phase system
downstream processing
continuous extraction equipment
protein
purification
solvent extraction
topic aqueous two-phase system
downstream processing
continuous extraction equipment
protein
purification
solvent extraction
description This work presents the results obtained using a microcolumn agitated by pulsed caps, using aqueous two-phase systems formed polyethylene glycol and salts (monobasic and dibasic) potassium phosphate for protein extraction. Proteins used were extracted cytochrome b5 and the enzyme ascorbic oxidoreductase. It was observed that operation of this equipment was stable and high efficiency values were achieved.
publishDate 2003
dc.date.none.fl_str_mv 2003-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000400003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-66322003000400003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0104-66322003000400003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
publisher.none.fl_str_mv Brazilian Society of Chemical Engineering
dc.source.none.fl_str_mv Brazilian Journal of Chemical Engineering v.20 n.4 2003
reponame:Brazilian Journal of Chemical Engineering
instname:Associação Brasileira de Engenharia Química (ABEQ)
instacron:ABEQ
instname_str Associação Brasileira de Engenharia Química (ABEQ)
instacron_str ABEQ
institution ABEQ
reponame_str Brazilian Journal of Chemical Engineering
collection Brazilian Journal of Chemical Engineering
repository.name.fl_str_mv Brazilian Journal of Chemical Engineering - Associação Brasileira de Engenharia Química (ABEQ)
repository.mail.fl_str_mv rgiudici@usp.br||rgiudici@usp.br
_version_ 1754213171499892736

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