In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista brasileira de hematologia e hemoterapia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000400005 |
Resumo: | Glyphosate [N-(phosphonomethyl)-glycine] is a broad-spectrum, non-selective, post-emergence herbicide that is extensively used in agriculture. Published data referring to the effects of this product on human health are contradictory. We showed previously that long-term treatment of rats with low doses of Glyphosate-Biocarb® may induce hepatic histological changes and bleeding without decreasing platelet counts. The aim of the current study was to investigate, in vitro, the effect of glyphosate on human blood platelet aggregation and coagulation. Materials and methods: Platelet aggregation was determined in the platelet-rich plasma using the agents: 6µM-adenosine diphosphate, 6µM-epinephrine and 4µg/mL-collagen. Pretreatment with 500µg/mL glyphosate showed significant hypofunction of the three aggregating agents. The inhibitory effect was dose-dependent at concentrations from 50 to 500 µg/mL. The release of ATP was lower for glyphosate-treated platelets after stimulation by collagen. On the other hand, glyphosate did not promote any inhibitory effects on prothrombin time, thromboplastin time and thrombin time. In conclusion, the results demonstrate that glyphosate promotes changes in the platelet metabolism with an inhibitory effect on primary hemostasis. |
id |
ABHHTC-1_357caa9a151680c31082bf6e4dbac1b0 |
---|---|
oai_identifier_str |
oai:scielo:S1516-84842010000400005 |
network_acronym_str |
ABHHTC-1 |
network_name_str |
Revista brasileira de hematologia e hemoterapia (Online) |
repository_id_str |
|
spelling |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulationGlyphosateplatelet aggregationplatelet secretionblood coagulationherbicide resistanceGlyphosate [N-(phosphonomethyl)-glycine] is a broad-spectrum, non-selective, post-emergence herbicide that is extensively used in agriculture. Published data referring to the effects of this product on human health are contradictory. We showed previously that long-term treatment of rats with low doses of Glyphosate-Biocarb® may induce hepatic histological changes and bleeding without decreasing platelet counts. The aim of the current study was to investigate, in vitro, the effect of glyphosate on human blood platelet aggregation and coagulation. Materials and methods: Platelet aggregation was determined in the platelet-rich plasma using the agents: 6µM-adenosine diphosphate, 6µM-epinephrine and 4µg/mL-collagen. Pretreatment with 500µg/mL glyphosate showed significant hypofunction of the three aggregating agents. The inhibitory effect was dose-dependent at concentrations from 50 to 500 µg/mL. The release of ATP was lower for glyphosate-treated platelets after stimulation by collagen. On the other hand, glyphosate did not promote any inhibitory effects on prothrombin time, thromboplastin time and thrombin time. In conclusion, the results demonstrate that glyphosate promotes changes in the platelet metabolism with an inhibitory effect on primary hemostasis.Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000400005Revista Brasileira de Hematologia e Hemoterapia v.32 n.4 2010reponame:Revista brasileira de hematologia e hemoterapia (Online)instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)instacron:ABHHTC10.1590/S1516-84842010005000087info:eu-repo/semantics/openAccessNeiva,Teresinha de Jesus C.Moraes,Ana Carolina R.Schwyzer,RafaellaVituri,Cidônia de LourdesRocha,Tania Rubia F.Fries,Diana M.Silva,Márcio A.Benedetti,Aloisio Luizeng2012-02-01T00:00:00Zoai:scielo:S1516-84842010000400005Revistahttp://www.rbhh.org/pt/archivo/https://old.scielo.br/oai/scielo-oai.phpsbhh@terra.com.br||secretaria@rbhh.org1806-08701516-8484opendoar:2012-02-01T00:00Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC)false |
dc.title.none.fl_str_mv |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
title |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
spellingShingle |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation Neiva,Teresinha de Jesus C. Glyphosate platelet aggregation platelet secretion blood coagulation herbicide resistance |
title_short |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
title_full |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
title_fullStr |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
title_full_unstemmed |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
title_sort |
In vitro effect of the herbicide glyphosate on human blood platelet aggregation and coagulation |
author |
Neiva,Teresinha de Jesus C. |
author_facet |
Neiva,Teresinha de Jesus C. Moraes,Ana Carolina R. Schwyzer,Rafaella Vituri,Cidônia de Lourdes Rocha,Tania Rubia F. Fries,Diana M. Silva,Márcio A. Benedetti,Aloisio Luiz |
author_role |
author |
author2 |
Moraes,Ana Carolina R. Schwyzer,Rafaella Vituri,Cidônia de Lourdes Rocha,Tania Rubia F. Fries,Diana M. Silva,Márcio A. Benedetti,Aloisio Luiz |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Neiva,Teresinha de Jesus C. Moraes,Ana Carolina R. Schwyzer,Rafaella Vituri,Cidônia de Lourdes Rocha,Tania Rubia F. Fries,Diana M. Silva,Márcio A. Benedetti,Aloisio Luiz |
dc.subject.por.fl_str_mv |
Glyphosate platelet aggregation platelet secretion blood coagulation herbicide resistance |
topic |
Glyphosate platelet aggregation platelet secretion blood coagulation herbicide resistance |
description |
Glyphosate [N-(phosphonomethyl)-glycine] is a broad-spectrum, non-selective, post-emergence herbicide that is extensively used in agriculture. Published data referring to the effects of this product on human health are contradictory. We showed previously that long-term treatment of rats with low doses of Glyphosate-Biocarb® may induce hepatic histological changes and bleeding without decreasing platelet counts. The aim of the current study was to investigate, in vitro, the effect of glyphosate on human blood platelet aggregation and coagulation. Materials and methods: Platelet aggregation was determined in the platelet-rich plasma using the agents: 6µM-adenosine diphosphate, 6µM-epinephrine and 4µg/mL-collagen. Pretreatment with 500µg/mL glyphosate showed significant hypofunction of the three aggregating agents. The inhibitory effect was dose-dependent at concentrations from 50 to 500 µg/mL. The release of ATP was lower for glyphosate-treated platelets after stimulation by collagen. On the other hand, glyphosate did not promote any inhibitory effects on prothrombin time, thromboplastin time and thrombin time. In conclusion, the results demonstrate that glyphosate promotes changes in the platelet metabolism with an inhibitory effect on primary hemostasis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000400005 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-84842010000400005 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-84842010005000087 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
publisher.none.fl_str_mv |
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular |
dc.source.none.fl_str_mv |
Revista Brasileira de Hematologia e Hemoterapia v.32 n.4 2010 reponame:Revista brasileira de hematologia e hemoterapia (Online) instname:Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC |
instname_str |
Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) |
instacron_str |
ABHHTC |
institution |
ABHHTC |
reponame_str |
Revista brasileira de hematologia e hemoterapia (Online) |
collection |
Revista brasileira de hematologia e hemoterapia (Online) |
repository.name.fl_str_mv |
Revista brasileira de hematologia e hemoterapia (Online) - Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) |
repository.mail.fl_str_mv |
sbhh@terra.com.br||secretaria@rbhh.org |
_version_ |
1754213111129178112 |