Cerebrospinal fluid can be used for HIV genotyping when it fails in blood

Detalhes bibliográficos
Autor(a) principal: Rotta,Indianara
Data de Publicação: 2014
Outros Autores: Raboni,Sonia Mara, Ribeiro,Cléa Elisa Lopes, Riedel,Maristela, Winhescki,Maria da Graça, Smith,Davey M., Ellis,Ronald J., Almeida,Sérgio Monteiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Arquivos de neuro-psiquiatria (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2014000700506
Resumo: Blood plasma specimens are the clinical standard for HIV-1 pol gene genotyping from viral populations; however, it is not always successful, often from low viral loads or the presence of polymerase chain reaction (PCR) inhibitors. Objective To describe the successful of HIV-1 genotyping in two samples of cerebrospinal fluid (CSF), after genotype procedures failed from blood. Method Two HIV-infected patients enrolled in a neurocognitive research study were evaluated when standard HIV-1 genotyping failed from blood plasma samples. Genotyping was performed using the commercial system TRUGENE® HIV-1 Genotyping Kit and the OpenGene® DNA Sequencing System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Results CSF genotyping was performed via the same commercial platform and was successful in both cases. Conclusion This report demonstrates that CSF could be used as an alternate clinical specimen for HIV-1 genotyping when it fails from blood.
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spelling Cerebrospinal fluid can be used for HIV genotyping when it fails in bloodHIV-1cladesgenotypingcerebrospinal fluidcentral nervous systemBlood plasma specimens are the clinical standard for HIV-1 pol gene genotyping from viral populations; however, it is not always successful, often from low viral loads or the presence of polymerase chain reaction (PCR) inhibitors. Objective To describe the successful of HIV-1 genotyping in two samples of cerebrospinal fluid (CSF), after genotype procedures failed from blood. Method Two HIV-infected patients enrolled in a neurocognitive research study were evaluated when standard HIV-1 genotyping failed from blood plasma samples. Genotyping was performed using the commercial system TRUGENE® HIV-1 Genotyping Kit and the OpenGene® DNA Sequencing System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Results CSF genotyping was performed via the same commercial platform and was successful in both cases. Conclusion This report demonstrates that CSF could be used as an alternate clinical specimen for HIV-1 genotyping when it fails from blood. Academia Brasileira de Neurologia - ABNEURO2014-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2014000700506Arquivos de Neuro-Psiquiatria v.72 n.7 2014reponame:Arquivos de neuro-psiquiatria (Online)instname:Academia Brasileira de Neurologiainstacron:ABNEURO10.1590/0004-282X20140093info:eu-repo/semantics/openAccessRotta,IndianaraRaboni,Sonia MaraRibeiro,Cléa Elisa LopesRiedel,MaristelaWinhescki,Maria da GraçaSmith,Davey M.Ellis,Ronald J.Almeida,Sérgio Monteiro deeng2014-07-14T00:00:00Zoai:scielo:S0004-282X2014000700506Revistahttp://www.scielo.br/anphttps://old.scielo.br/oai/scielo-oai.php||revista.arquivos@abneuro.org1678-42270004-282Xopendoar:2014-07-14T00:00Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologiafalse
dc.title.none.fl_str_mv Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
title Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
spellingShingle Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
Rotta,Indianara
HIV-1
clades
genotyping
cerebrospinal fluid
central nervous system
title_short Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
title_full Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
title_fullStr Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
title_full_unstemmed Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
title_sort Cerebrospinal fluid can be used for HIV genotyping when it fails in blood
author Rotta,Indianara
author_facet Rotta,Indianara
Raboni,Sonia Mara
Ribeiro,Cléa Elisa Lopes
Riedel,Maristela
Winhescki,Maria da Graça
Smith,Davey M.
Ellis,Ronald J.
Almeida,Sérgio Monteiro de
author_role author
author2 Raboni,Sonia Mara
Ribeiro,Cléa Elisa Lopes
Riedel,Maristela
Winhescki,Maria da Graça
Smith,Davey M.
Ellis,Ronald J.
Almeida,Sérgio Monteiro de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rotta,Indianara
Raboni,Sonia Mara
Ribeiro,Cléa Elisa Lopes
Riedel,Maristela
Winhescki,Maria da Graça
Smith,Davey M.
Ellis,Ronald J.
Almeida,Sérgio Monteiro de
dc.subject.por.fl_str_mv HIV-1
clades
genotyping
cerebrospinal fluid
central nervous system
topic HIV-1
clades
genotyping
cerebrospinal fluid
central nervous system
description Blood plasma specimens are the clinical standard for HIV-1 pol gene genotyping from viral populations; however, it is not always successful, often from low viral loads or the presence of polymerase chain reaction (PCR) inhibitors. Objective To describe the successful of HIV-1 genotyping in two samples of cerebrospinal fluid (CSF), after genotype procedures failed from blood. Method Two HIV-infected patients enrolled in a neurocognitive research study were evaluated when standard HIV-1 genotyping failed from blood plasma samples. Genotyping was performed using the commercial system TRUGENE® HIV-1 Genotyping Kit and the OpenGene® DNA Sequencing System (Siemens Healthcare Diagnostics, Tarrytown, NY, USA). Results CSF genotyping was performed via the same commercial platform and was successful in both cases. Conclusion This report demonstrates that CSF could be used as an alternate clinical specimen for HIV-1 genotyping when it fails from blood.
publishDate 2014
dc.date.none.fl_str_mv 2014-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2014000700506
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0004-282X2014000700506
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/0004-282X20140093
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
publisher.none.fl_str_mv Academia Brasileira de Neurologia - ABNEURO
dc.source.none.fl_str_mv Arquivos de Neuro-Psiquiatria v.72 n.7 2014
reponame:Arquivos de neuro-psiquiatria (Online)
instname:Academia Brasileira de Neurologia
instacron:ABNEURO
instname_str Academia Brasileira de Neurologia
instacron_str ABNEURO
institution ABNEURO
reponame_str Arquivos de neuro-psiquiatria (Online)
collection Arquivos de neuro-psiquiatria (Online)
repository.name.fl_str_mv Arquivos de neuro-psiquiatria (Online) - Academia Brasileira de Neurologia
repository.mail.fl_str_mv ||revista.arquivos@abneuro.org
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