Inhibitory effect of mouthwashes on Lactobacilus casei growth
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista Brasileira de Odontologia |
Texto Completo: | https://revista.aborj.org.br/index.php/rbo/article/view/285 |
Resumo: | This study assessed the inhibitory effect of commercially available mouth rinses on the growth of Lactobacillus casei (ATCC 7469). An inductive approach was adopted with a comparative procedure and the technique of direct observation in the laboratory. The sample consisted of six products (Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ®) which were analyzed to measure their Minimum Inhibitory Concentration (MIC) on Blood Agar solid culture medium. Serial dilutions of the mouthwash were carried out, from the commercially available solution, resulting in seven concentrations (1:0 to 1:32). A quantity of 50?L of each solution and their respective dilutions were inserted in wells (6 mm) made in the culture medium. The plates were incubated in a bacteriological incubator at 37 ° C for 24 hours. The halos of growth inhibition were measured and the MIC was considered to be the last concentration at which formation of halos greater than or equal to 10mm were observed. The MIC of the mouthwashes Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ® were measured at concentrations of 1:2, 1:8, 1:4, 1:8, 1:16, and 1:32, respectively. It was concluded that the mouthwashes studied presented antimicrobial activity against L. casei, and the largest inhibitory effect was with Periogard ® and Sanifill ®. |
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Inhibitory effect of mouthwashes on Lactobacilus casei growthEfeito inibitório de enxaguatórios bucais sobre o crescimento de Lactobacilos caseiMicrobiologiaMicrobiologia; Testes de Sensibilidade Microbiana; Lactobacillus casei; Microbiology; Microbial Sensitivity Tests;This study assessed the inhibitory effect of commercially available mouth rinses on the growth of Lactobacillus casei (ATCC 7469). An inductive approach was adopted with a comparative procedure and the technique of direct observation in the laboratory. The sample consisted of six products (Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ®) which were analyzed to measure their Minimum Inhibitory Concentration (MIC) on Blood Agar solid culture medium. Serial dilutions of the mouthwash were carried out, from the commercially available solution, resulting in seven concentrations (1:0 to 1:32). A quantity of 50?L of each solution and their respective dilutions were inserted in wells (6 mm) made in the culture medium. The plates were incubated in a bacteriological incubator at 37 ° C for 24 hours. The halos of growth inhibition were measured and the MIC was considered to be the last concentration at which formation of halos greater than or equal to 10mm were observed. The MIC of the mouthwashes Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ® were measured at concentrations of 1:2, 1:8, 1:4, 1:8, 1:16, and 1:32, respectively. It was concluded that the mouthwashes studied presented antimicrobial activity against L. casei, and the largest inhibitory effect was with Periogard ® and Sanifill ®.Este estudo avaliou efeito inibitório de enxaguatórios bucais disponíveis comercialmente sobre o crescimento de Lactobacilos casei (ATCC 7469). Adotou-se uma abordagem indutiva com procedimento comparativo e técnica de observação direta em laboratório. A amostra foi composta por seis produtos (Plax®, Listerine®, Cepacol®, Oral B®, Sanifill® e Periogard®) os quais foram analisados quanto à determinação da Concentração Inibitória Mínima (CIM) em meio de cultura sólido agar Sangue. Realizou-se diluição seriada dos enxaguatórios, a partir da solução pronta para uso, resultando em sete concentrações (1:0 até 1:32). Foram inseridos 50µL de cada solução pronta para uso, bem como de suas respectivas diluições, nos poços (6mm) confeccionados no meio de cultura. As placas foram incubadas em estufa bacteriológica a 37°C por 24h. Os halos de inibição do crescimento forma mensurados e a CIM foi considerada a última concentração na qual se observou formação de halos iguais ou superiores a 10mm. A CIM dos enxaguatórios bucais Plax®, Listerine®, Cepacol®, Oral B®, Sanifill® e Periogard® foi verificada, respectivamente, nas concentrações 1:2, 1:8, 1:4, 1:8, 1:16 e 1:32. Concluiu-se que os enxaguatórios bucais estudados apresentaram ação antimicrobiana frente a L. casei, sendo o maior efeito inibitório observado para o Periogard® e Sanifill®.Associação Brasileira de Odontologia - Seção Rio de Janeiro2012-08-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revista.aborj.org.br/index.php/rbo/article/view/28510.18363/rbo.v69n1.p.107Revista Brasileira de Odontologia; v. 69, n. 1 (2012); 107Revista Brasileira de Odontologia; v. 69, n. 1 (2012); 1071984-3747reponame:Revista Brasileira de Odontologiainstname:Associação Brasileira de Odontologia (ABO)instacron:ABOporhttps://revista.aborj.org.br/index.php/rbo/article/view/285/287Direitos autorais 2016 Revistasinfo:eu-repo/semantics/openAccessRamos, Ianny AlvesBastos Leite, RafaellaMenezes, Karyna de MeloJovito, Vanessa CarvalhoAlmeida, Leopoldina de Fátima Dantas deCavalcanti, Yuri WanderleyCastro, Ricardo Dias deCavalcanti, Alessandro Leite2012-09-27T15:50:19Zoai:revista_aborj_org_br.www.revista.aborj.org.br:article/285Revistahttps://revista.aborj.org.br/index.php/rboONGhttp://www.revista.aborj.org.br/index.php/rbo/oaihttps://revista.aborj.org.br/index.php/rbo/oai0034-72721984-3747opendoar:2012-09-27T15:50:19Revista Brasileira de Odontologia - Associação Brasileira de Odontologia (ABO)false |
dc.title.none.fl_str_mv |
Inhibitory effect of mouthwashes on Lactobacilus casei growth Efeito inibitório de enxaguatórios bucais sobre o crescimento de Lactobacilos casei |
title |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
spellingShingle |
Inhibitory effect of mouthwashes on Lactobacilus casei growth Ramos, Ianny Alves Microbiologia Microbiologia; Testes de Sensibilidade Microbiana; Lactobacillus casei; Microbiology; Microbial Sensitivity Tests; |
title_short |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
title_full |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
title_fullStr |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
title_full_unstemmed |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
title_sort |
Inhibitory effect of mouthwashes on Lactobacilus casei growth |
author |
Ramos, Ianny Alves |
author_facet |
Ramos, Ianny Alves Bastos Leite, Rafaella Menezes, Karyna de Melo Jovito, Vanessa Carvalho Almeida, Leopoldina de Fátima Dantas de Cavalcanti, Yuri Wanderley Castro, Ricardo Dias de Cavalcanti, Alessandro Leite |
author_role |
author |
author2 |
Bastos Leite, Rafaella Menezes, Karyna de Melo Jovito, Vanessa Carvalho Almeida, Leopoldina de Fátima Dantas de Cavalcanti, Yuri Wanderley Castro, Ricardo Dias de Cavalcanti, Alessandro Leite |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Ramos, Ianny Alves Bastos Leite, Rafaella Menezes, Karyna de Melo Jovito, Vanessa Carvalho Almeida, Leopoldina de Fátima Dantas de Cavalcanti, Yuri Wanderley Castro, Ricardo Dias de Cavalcanti, Alessandro Leite |
dc.subject.por.fl_str_mv |
Microbiologia Microbiologia; Testes de Sensibilidade Microbiana; Lactobacillus casei; Microbiology; Microbial Sensitivity Tests; |
topic |
Microbiologia Microbiologia; Testes de Sensibilidade Microbiana; Lactobacillus casei; Microbiology; Microbial Sensitivity Tests; |
description |
This study assessed the inhibitory effect of commercially available mouth rinses on the growth of Lactobacillus casei (ATCC 7469). An inductive approach was adopted with a comparative procedure and the technique of direct observation in the laboratory. The sample consisted of six products (Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ®) which were analyzed to measure their Minimum Inhibitory Concentration (MIC) on Blood Agar solid culture medium. Serial dilutions of the mouthwash were carried out, from the commercially available solution, resulting in seven concentrations (1:0 to 1:32). A quantity of 50?L of each solution and their respective dilutions were inserted in wells (6 mm) made in the culture medium. The plates were incubated in a bacteriological incubator at 37 ° C for 24 hours. The halos of growth inhibition were measured and the MIC was considered to be the last concentration at which formation of halos greater than or equal to 10mm were observed. The MIC of the mouthwashes Plax ®, Listerine ®, Cepacol ®, Oral B ®, Sanifill ® and Periogard ® were measured at concentrations of 1:2, 1:8, 1:4, 1:8, 1:16, and 1:32, respectively. It was concluded that the mouthwashes studied presented antimicrobial activity against L. casei, and the largest inhibitory effect was with Periogard ® and Sanifill ®. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-15 |
dc.type.none.fl_str_mv |
|
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://revista.aborj.org.br/index.php/rbo/article/view/285 10.18363/rbo.v69n1.p.107 |
url |
https://revista.aborj.org.br/index.php/rbo/article/view/285 |
identifier_str_mv |
10.18363/rbo.v69n1.p.107 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://revista.aborj.org.br/index.php/rbo/article/view/285/287 |
dc.rights.driver.fl_str_mv |
Direitos autorais 2016 Revistas info:eu-repo/semantics/openAccess |
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Direitos autorais 2016 Revistas |
eu_rights_str_mv |
openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Odontologia - Seção Rio de Janeiro |
publisher.none.fl_str_mv |
Associação Brasileira de Odontologia - Seção Rio de Janeiro |
dc.source.none.fl_str_mv |
Revista Brasileira de Odontologia; v. 69, n. 1 (2012); 107 Revista Brasileira de Odontologia; v. 69, n. 1 (2012); 107 1984-3747 reponame:Revista Brasileira de Odontologia instname:Associação Brasileira de Odontologia (ABO) instacron:ABO |
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Associação Brasileira de Odontologia (ABO) |
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ABO |
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ABO |
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Revista Brasileira de Odontologia |
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Revista Brasileira de Odontologia |
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Revista Brasileira de Odontologia - Associação Brasileira de Odontologia (ABO) |
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https://revista.aborj.org.br/index.php/rbo/oai |
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