Antimicrobial activity in vitro of latex from Jatropha multifida L.

Detalhes bibliográficos
Autor(a) principal: Vieira, Daniglayse Santos
Data de Publicação: 2021
Outros Autores: Basílio Junior, Irinaldo Diniz, Moreira, Rossana Teotonio de Farias, Calumby, Rodrigo José Nunes, Silva, Rafael Aleixo dos Santos, Silva, Valdemir da Costa, Bastos, Maria Lysete de Assis
Tipo de documento: Artigo
Idioma: por
Título da fonte: Research, Society and Development
Texto Completo: https://rsdjournal.org/index.php/rsd/article/view/17511
Resumo: Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies.
id UNIFEI_b80221f4c1490843c4aea5209b461fb1
oai_identifier_str oai:ojs.pkp.sfu.ca:article/17511
network_acronym_str UNIFEI
network_name_str Research, Society and Development
repository_id_str
spelling Antimicrobial activity in vitro of latex from Jatropha multifida L.Actividad antimicrobiana in vitro del latex de Jatropha multifida L.Atividade antimicrobiana in vitro do látex da Jatropha multifida L. MicrobiologíaPlanta medicinalPruebas de sensibilidad microbiana.MicrobiologyMedicinal plantMicrobial sensitivity tests.MicrobiologiaPlanta medicinalTestes de sensibilidade microbiana. Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies.Jatropha multifida L., conocida popularmente como mertiolate, es un arbusto cuyas flores rojas con anteras amarillentas parecen pequeños trozos de coral y un tallo con presencia de látex. Se utiliza en la medicina popular para tratar diversas afecciones, como heridas infectadas, úlceras y dermatosis. El objetivo del estudio fue verificar la actividad antimicrobiana del látex in natura y liofilizado de J. multifida. Esta es una investigación cuantitativa, de tipo experimental. El látex fue recolectado en la ciudad de Igreja Nova - Alagoas, mediante el método de sangrado, en el que se realizaban cortes oblicuos en el tallo, obteniendo el extracto in natura. Para la obtención del extracto en polvo se utilizó la liofilización de la materia prima. Para evaluar la actividad antimicrobiana, se utilizó el método de microdilución en caldo para determinar la Concentración Inhibitoria Mínima (MIC), seguido de plaqueamiento en agar para obtener la Concentración Bactericida Mínima (CBM) de los extractos contra los microorganismos estandarizados por la American Type Culture Collection - ATCC: S. aureus (25923), S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) y Concentración Fungicida Mínima (MFM) para C. albicans (24433). Los resultados evidenciaron que ambos extractos de látex de J. multifida presentaron una actividad antimicrobiana moderada contra la mayoría de las cepas bacterianas probadas y una actividad fúngica débil contra C. albicans, con énfasis en el extracto liofilizado. Se concluye que los extractos fueron más prometedores contra cepas de S. aureus, S. epidermidis, E. faecalis y P. aeruginosa, lo que abre posibilidades para nuevos estudios.Jatropha multifida L., conhecida popularmente como mertiolate, é um arbusto, cujas flores vermelhas com anteras amareladas, parecem pequenas peças de coral e caule com presença de látex. É usada na medicina popular para tratar diversas afecções, como feridas infectadas, úlceras e dermatoses. O objetivo do estudo foi verificar a atividade antimicrobiana do látex in natura e liofilizado da J. multifida. Trata-se de pesquisa quantitativa, do tipo experimental. O látex foi coletado no município de Igreja Nova – Alagoas, utilizando-se o método de sangria, em que se realizou cortes oblíquos no caule, obtendo-se o extrato in natura. Já para obtenção do extrato em pó, utilizou-se a liofilização do material in natura. Para avaliação da atividade antimicrobiana foi empregado o método de microdiluição em caldo para determinação da Concentração Inibitória Mínima (CIM), seguido de plaqueamento em ágar para obtenção da Concentração Bactericida Mínima (CBM) dos extratos frente aos microrganismos padronizados pela American Type Culture Collection – ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) e Concentração Fungicida Mínima (CFM) para C. albicans (24433). Os resultados apontaram que ambos os extratos do látex da J. multifida apresentaram uma atividade antimicrobiana moderada contra a maioria das cepas bacterianas testadas e uma atividade fúngica fraca frente a C. albicans, com destaque para o extrato liofilizado. Conclui-se que os extratos foram mais promissores contra as cepas de S. aureus, S. epidermidis, E. faecalis e P. aeruginosa, abrindo possibilidades para evolução de estudos complementares.Research, Society and Development2021-08-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1751110.33448/rsd-v10i10.17511Research, Society and Development; Vol. 10 No. 10; e334101017511Research, Society and Development; Vol. 10 Núm. 10; e334101017511Research, Society and Development; v. 10 n. 10; e3341010175112525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17511/16878Copyright (c) 2021 Daniglayse Santos Vieira; Irinaldo Diniz Basílio Junior; Rossana Teotonio de Farias Moreira; Rodrigo José Nunes Calumby; Rafael Aleixo dos Santos Silva; Valdemir da Costa Silva; Maria Lysete de Assis Bastoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVieira, Daniglayse Santos Basílio Junior, Irinaldo Diniz Moreira, Rossana Teotonio de Farias Calumby, Rodrigo José Nunes Silva, Rafael Aleixo dos Santos Silva, Valdemir da Costa Bastos, Maria Lysete de Assis 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/17511Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:50.142237Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false
dc.title.none.fl_str_mv Antimicrobial activity in vitro of latex from Jatropha multifida L.
Actividad antimicrobiana in vitro del latex de Jatropha multifida L.
Atividade antimicrobiana in vitro do látex da Jatropha multifida L.
title Antimicrobial activity in vitro of latex from Jatropha multifida L.
spellingShingle Antimicrobial activity in vitro of latex from Jatropha multifida L.
Vieira, Daniglayse Santos
Microbiología
Planta medicinal
Pruebas de sensibilidad microbiana.
Microbiology
Medicinal plant
Microbial sensitivity tests.
Microbiologia
Planta medicinal
Testes de sensibilidade microbiana.
title_short Antimicrobial activity in vitro of latex from Jatropha multifida L.
title_full Antimicrobial activity in vitro of latex from Jatropha multifida L.
title_fullStr Antimicrobial activity in vitro of latex from Jatropha multifida L.
title_full_unstemmed Antimicrobial activity in vitro of latex from Jatropha multifida L.
title_sort Antimicrobial activity in vitro of latex from Jatropha multifida L.
author Vieira, Daniglayse Santos
author_facet Vieira, Daniglayse Santos
Basílio Junior, Irinaldo Diniz
Moreira, Rossana Teotonio de Farias
Calumby, Rodrigo José Nunes
Silva, Rafael Aleixo dos Santos
Silva, Valdemir da Costa
Bastos, Maria Lysete de Assis
author_role author
author2 Basílio Junior, Irinaldo Diniz
Moreira, Rossana Teotonio de Farias
Calumby, Rodrigo José Nunes
Silva, Rafael Aleixo dos Santos
Silva, Valdemir da Costa
Bastos, Maria Lysete de Assis
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Daniglayse Santos
Basílio Junior, Irinaldo Diniz
Moreira, Rossana Teotonio de Farias
Calumby, Rodrigo José Nunes
Silva, Rafael Aleixo dos Santos
Silva, Valdemir da Costa
Bastos, Maria Lysete de Assis
dc.subject.por.fl_str_mv Microbiología
Planta medicinal
Pruebas de sensibilidad microbiana.
Microbiology
Medicinal plant
Microbial sensitivity tests.
Microbiologia
Planta medicinal
Testes de sensibilidade microbiana.
topic Microbiología
Planta medicinal
Pruebas de sensibilidad microbiana.
Microbiology
Medicinal plant
Microbial sensitivity tests.
Microbiologia
Planta medicinal
Testes de sensibilidade microbiana.
description Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-12
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17511
10.33448/rsd-v10i10.17511
url https://rsdjournal.org/index.php/rsd/article/view/17511
identifier_str_mv 10.33448/rsd-v10i10.17511
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rsdjournal.org/index.php/rsd/article/view/17511/16878
dc.rights.driver.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Research, Society and Development
publisher.none.fl_str_mv Research, Society and Development
dc.source.none.fl_str_mv Research, Society and Development; Vol. 10 No. 10; e334101017511
Research, Society and Development; Vol. 10 Núm. 10; e334101017511
Research, Society and Development; v. 10 n. 10; e334101017511
2525-3409
reponame:Research, Society and Development
instname:Universidade Federal de Itajubá (UNIFEI)
instacron:UNIFEI
instname_str Universidade Federal de Itajubá (UNIFEI)
instacron_str UNIFEI
institution UNIFEI
reponame_str Research, Society and Development
collection Research, Society and Development
repository.name.fl_str_mv Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)
repository.mail.fl_str_mv rsd.articles@gmail.com
_version_ 1797052833182253056