Antimicrobial activity in vitro of latex from Jatropha multifida L.
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Research, Society and Development |
Texto Completo: | https://rsdjournal.org/index.php/rsd/article/view/17511 |
Resumo: | Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies. |
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Antimicrobial activity in vitro of latex from Jatropha multifida L.Actividad antimicrobiana in vitro del latex de Jatropha multifida L.Atividade antimicrobiana in vitro do látex da Jatropha multifida L. MicrobiologíaPlanta medicinalPruebas de sensibilidad microbiana.MicrobiologyMedicinal plantMicrobial sensitivity tests.MicrobiologiaPlanta medicinalTestes de sensibilidade microbiana. Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies.Jatropha multifida L., conocida popularmente como mertiolate, es un arbusto cuyas flores rojas con anteras amarillentas parecen pequeños trozos de coral y un tallo con presencia de látex. Se utiliza en la medicina popular para tratar diversas afecciones, como heridas infectadas, úlceras y dermatosis. El objetivo del estudio fue verificar la actividad antimicrobiana del látex in natura y liofilizado de J. multifida. Esta es una investigación cuantitativa, de tipo experimental. El látex fue recolectado en la ciudad de Igreja Nova - Alagoas, mediante el método de sangrado, en el que se realizaban cortes oblicuos en el tallo, obteniendo el extracto in natura. Para la obtención del extracto en polvo se utilizó la liofilización de la materia prima. Para evaluar la actividad antimicrobiana, se utilizó el método de microdilución en caldo para determinar la Concentración Inhibitoria Mínima (MIC), seguido de plaqueamiento en agar para obtener la Concentración Bactericida Mínima (CBM) de los extractos contra los microorganismos estandarizados por la American Type Culture Collection - ATCC: S. aureus (25923), S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) y Concentración Fungicida Mínima (MFM) para C. albicans (24433). Los resultados evidenciaron que ambos extractos de látex de J. multifida presentaron una actividad antimicrobiana moderada contra la mayoría de las cepas bacterianas probadas y una actividad fúngica débil contra C. albicans, con énfasis en el extracto liofilizado. Se concluye que los extractos fueron más prometedores contra cepas de S. aureus, S. epidermidis, E. faecalis y P. aeruginosa, lo que abre posibilidades para nuevos estudios.Jatropha multifida L., conhecida popularmente como mertiolate, é um arbusto, cujas flores vermelhas com anteras amareladas, parecem pequenas peças de coral e caule com presença de látex. É usada na medicina popular para tratar diversas afecções, como feridas infectadas, úlceras e dermatoses. O objetivo do estudo foi verificar a atividade antimicrobiana do látex in natura e liofilizado da J. multifida. Trata-se de pesquisa quantitativa, do tipo experimental. O látex foi coletado no município de Igreja Nova – Alagoas, utilizando-se o método de sangria, em que se realizou cortes oblíquos no caule, obtendo-se o extrato in natura. Já para obtenção do extrato em pó, utilizou-se a liofilização do material in natura. Para avaliação da atividade antimicrobiana foi empregado o método de microdiluição em caldo para determinação da Concentração Inibitória Mínima (CIM), seguido de plaqueamento em ágar para obtenção da Concentração Bactericida Mínima (CBM) dos extratos frente aos microrganismos padronizados pela American Type Culture Collection – ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) e Concentração Fungicida Mínima (CFM) para C. albicans (24433). Os resultados apontaram que ambos os extratos do látex da J. multifida apresentaram uma atividade antimicrobiana moderada contra a maioria das cepas bacterianas testadas e uma atividade fúngica fraca frente a C. albicans, com destaque para o extrato liofilizado. Conclui-se que os extratos foram mais promissores contra as cepas de S. aureus, S. epidermidis, E. faecalis e P. aeruginosa, abrindo possibilidades para evolução de estudos complementares.Research, Society and Development2021-08-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://rsdjournal.org/index.php/rsd/article/view/1751110.33448/rsd-v10i10.17511Research, Society and Development; Vol. 10 No. 10; e334101017511Research, Society and Development; Vol. 10 Núm. 10; e334101017511Research, Society and Development; v. 10 n. 10; e3341010175112525-3409reponame:Research, Society and Developmentinstname:Universidade Federal de Itajubá (UNIFEI)instacron:UNIFEIporhttps://rsdjournal.org/index.php/rsd/article/view/17511/16878Copyright (c) 2021 Daniglayse Santos Vieira; Irinaldo Diniz Basílio Junior; Rossana Teotonio de Farias Moreira; Rodrigo José Nunes Calumby; Rafael Aleixo dos Santos Silva; Valdemir da Costa Silva; Maria Lysete de Assis Bastoshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessVieira, Daniglayse Santos Basílio Junior, Irinaldo Diniz Moreira, Rossana Teotonio de Farias Calumby, Rodrigo José Nunes Silva, Rafael Aleixo dos Santos Silva, Valdemir da Costa Bastos, Maria Lysete de Assis 2021-10-02T21:49:16Zoai:ojs.pkp.sfu.ca:article/17511Revistahttps://rsdjournal.org/index.php/rsd/indexPUBhttps://rsdjournal.org/index.php/rsd/oairsd.articles@gmail.com2525-34092525-3409opendoar:2024-01-17T09:37:50.142237Research, Society and Development - Universidade Federal de Itajubá (UNIFEI)false |
dc.title.none.fl_str_mv |
Antimicrobial activity in vitro of latex from Jatropha multifida L. Actividad antimicrobiana in vitro del latex de Jatropha multifida L. Atividade antimicrobiana in vitro do látex da Jatropha multifida L. |
title |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
spellingShingle |
Antimicrobial activity in vitro of latex from Jatropha multifida L. Vieira, Daniglayse Santos Microbiología Planta medicinal Pruebas de sensibilidad microbiana. Microbiology Medicinal plant Microbial sensitivity tests. Microbiologia Planta medicinal Testes de sensibilidade microbiana. |
title_short |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
title_full |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
title_fullStr |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
title_full_unstemmed |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
title_sort |
Antimicrobial activity in vitro of latex from Jatropha multifida L. |
author |
Vieira, Daniglayse Santos |
author_facet |
Vieira, Daniglayse Santos Basílio Junior, Irinaldo Diniz Moreira, Rossana Teotonio de Farias Calumby, Rodrigo José Nunes Silva, Rafael Aleixo dos Santos Silva, Valdemir da Costa Bastos, Maria Lysete de Assis |
author_role |
author |
author2 |
Basílio Junior, Irinaldo Diniz Moreira, Rossana Teotonio de Farias Calumby, Rodrigo José Nunes Silva, Rafael Aleixo dos Santos Silva, Valdemir da Costa Bastos, Maria Lysete de Assis |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Daniglayse Santos Basílio Junior, Irinaldo Diniz Moreira, Rossana Teotonio de Farias Calumby, Rodrigo José Nunes Silva, Rafael Aleixo dos Santos Silva, Valdemir da Costa Bastos, Maria Lysete de Assis |
dc.subject.por.fl_str_mv |
Microbiología Planta medicinal Pruebas de sensibilidad microbiana. Microbiology Medicinal plant Microbial sensitivity tests. Microbiologia Planta medicinal Testes de sensibilidade microbiana. |
topic |
Microbiología Planta medicinal Pruebas de sensibilidad microbiana. Microbiology Medicinal plant Microbial sensitivity tests. Microbiologia Planta medicinal Testes de sensibilidade microbiana. |
description |
Jatropha multifida L., popularly known as mertiolate, is a shrub whose red flowers with yellowish anthers look like small pieces of coral and a stem with latex presence. It is used in folk medicine to treat a variety of conditions, such as infected wounds, ulcers and dermatoses. The aim of the study was to verify the antimicrobial activity of in natura and lyophilized latex from J. multifida. It is a quantitative research, of the experimental type. The latex was collected in the city of Igreja Nova - Alagoas, using the bleeding method, in which oblique cuts were made on the stem, obtaining the extract in natura. To obtain the powdered extract, the lyophilization of the raw material was used. To evaluate the antimicrobial activity, the broth microdilution method was used to determine the Minimum Inhibitory Concentration (MIC), followed by plating on agar to obtain the Minimum Bactericidal Concentration (CBM) of the extracts against the microorganisms standardized by the American Type Culture Collection - ATCC: S. aureus (25923) S. epidermidis (31488), E. faecalis (29212), P. aeruginosa (27853), E. coli (25922), E. aerogenes (13048) and Minimum Fungicidal Concentration (MFM) for C . albicans (24433). The results showed that both J. multifida latex extracts showed moderate antimicrobial activity against most of the bacterial strains tested and a weak fungal activity against C. albicans, with emphasis on the lyophilized extract. It is concluded that the extracts were more promising against strains of S. aureus, S. epidermidis, E. faecalis and P. aeruginosa, opening up possibilities for further studies. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08-12 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17511 10.33448/rsd-v10i10.17511 |
url |
https://rsdjournal.org/index.php/rsd/article/view/17511 |
identifier_str_mv |
10.33448/rsd-v10i10.17511 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rsdjournal.org/index.php/rsd/article/view/17511/16878 |
dc.rights.driver.fl_str_mv |
https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Research, Society and Development |
publisher.none.fl_str_mv |
Research, Society and Development |
dc.source.none.fl_str_mv |
Research, Society and Development; Vol. 10 No. 10; e334101017511 Research, Society and Development; Vol. 10 Núm. 10; e334101017511 Research, Society and Development; v. 10 n. 10; e334101017511 2525-3409 reponame:Research, Society and Development instname:Universidade Federal de Itajubá (UNIFEI) instacron:UNIFEI |
instname_str |
Universidade Federal de Itajubá (UNIFEI) |
instacron_str |
UNIFEI |
institution |
UNIFEI |
reponame_str |
Research, Society and Development |
collection |
Research, Society and Development |
repository.name.fl_str_mv |
Research, Society and Development - Universidade Federal de Itajubá (UNIFEI) |
repository.mail.fl_str_mv |
rsd.articles@gmail.com |
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1797052833182253056 |