In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Polímeros (São Carlos. Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282017000100083 |
Resumo: | Abstract The importance of monitoring in vivo interaction that occurs between cells /bio/tissue recipient in the understanding of tissue regeneration processes becomes ever greater. This study aims to monitor and evaluate the influence of scaffold implants of poly (L-co-D, L lactic acid) - PLDLA synthesized in the laboratory, previously cultured with primary osteoblastic cells heterologously stained with the fluorescent vital dye, PKH26, on the tissue regeneration process in 8 mm central critical defects of the Wistar rat calvaria. The results obtained by MTT assay and monitoring of cells stained with PKH26 dye over 14 days of culture showed that the dye was cytocompatible with osteoblastic cells and did not exert a negative influence on the growth of unstained cells. In the in vivo study, macroscopic observations made during deployment times corroborate the results in vitro, as no apparent signs of toxicity were observed in the implanted bone defect area. The use of mobile monitoring with the dye, PKH26 in vivo is an effective strategy for the understanding of cell behaviour in the presence of PLDLA polymer. |
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In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffoldsPKH26PLDLAscaffoldstissue engineeringAbstract The importance of monitoring in vivo interaction that occurs between cells /bio/tissue recipient in the understanding of tissue regeneration processes becomes ever greater. This study aims to monitor and evaluate the influence of scaffold implants of poly (L-co-D, L lactic acid) - PLDLA synthesized in the laboratory, previously cultured with primary osteoblastic cells heterologously stained with the fluorescent vital dye, PKH26, on the tissue regeneration process in 8 mm central critical defects of the Wistar rat calvaria. The results obtained by MTT assay and monitoring of cells stained with PKH26 dye over 14 days of culture showed that the dye was cytocompatible with osteoblastic cells and did not exert a negative influence on the growth of unstained cells. In the in vivo study, macroscopic observations made during deployment times corroborate the results in vitro, as no apparent signs of toxicity were observed in the implanted bone defect area. The use of mobile monitoring with the dye, PKH26 in vivo is an effective strategy for the understanding of cell behaviour in the presence of PLDLA polymer.Associação Brasileira de Polímeros2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282017000100083Polímeros v.27 n.1 2017reponame:Polímeros (São Carlos. Online)instname:Associação Brasileira de Polímeros (ABPol)instacron:ABPO10.1590/0104-1428.2372info:eu-repo/semantics/openAccessDuek,Alice RezendeCosta,Gabriel Ciambelli Dias daMás,Bruna AntunesBarbo,Maria Lourdes PerisMotta,Adriana CristinaDuek,Eliana Aparecida de Rezendeeng2017-06-20T00:00:00Zoai:scielo:S0104-14282017000100083Revistahttp://www.scielo.br/pohttps://old.scielo.br/oai/scielo-oai.php||revista@abpol.org.br1678-51690104-1428opendoar:2017-06-20T00:00Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol)false |
dc.title.none.fl_str_mv |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
title |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
spellingShingle |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds Duek,Alice Rezende PKH26 PLDLA scaffolds tissue engineering |
title_short |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
title_full |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
title_fullStr |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
title_full_unstemmed |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
title_sort |
In vitro and in vivo cell tracking of PKH26-labeled osteoblasts cultured on PLDLA scaffolds |
author |
Duek,Alice Rezende |
author_facet |
Duek,Alice Rezende Costa,Gabriel Ciambelli Dias da Más,Bruna Antunes Barbo,Maria Lourdes Peris Motta,Adriana Cristina Duek,Eliana Aparecida de Rezende |
author_role |
author |
author2 |
Costa,Gabriel Ciambelli Dias da Más,Bruna Antunes Barbo,Maria Lourdes Peris Motta,Adriana Cristina Duek,Eliana Aparecida de Rezende |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Duek,Alice Rezende Costa,Gabriel Ciambelli Dias da Más,Bruna Antunes Barbo,Maria Lourdes Peris Motta,Adriana Cristina Duek,Eliana Aparecida de Rezende |
dc.subject.por.fl_str_mv |
PKH26 PLDLA scaffolds tissue engineering |
topic |
PKH26 PLDLA scaffolds tissue engineering |
description |
Abstract The importance of monitoring in vivo interaction that occurs between cells /bio/tissue recipient in the understanding of tissue regeneration processes becomes ever greater. This study aims to monitor and evaluate the influence of scaffold implants of poly (L-co-D, L lactic acid) - PLDLA synthesized in the laboratory, previously cultured with primary osteoblastic cells heterologously stained with the fluorescent vital dye, PKH26, on the tissue regeneration process in 8 mm central critical defects of the Wistar rat calvaria. The results obtained by MTT assay and monitoring of cells stained with PKH26 dye over 14 days of culture showed that the dye was cytocompatible with osteoblastic cells and did not exert a negative influence on the growth of unstained cells. In the in vivo study, macroscopic observations made during deployment times corroborate the results in vitro, as no apparent signs of toxicity were observed in the implanted bone defect area. The use of mobile monitoring with the dye, PKH26 in vivo is an effective strategy for the understanding of cell behaviour in the presence of PLDLA polymer. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282017000100083 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-14282017000100083 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/0104-1428.2372 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
publisher.none.fl_str_mv |
Associação Brasileira de Polímeros |
dc.source.none.fl_str_mv |
Polímeros v.27 n.1 2017 reponame:Polímeros (São Carlos. Online) instname:Associação Brasileira de Polímeros (ABPol) instacron:ABPO |
instname_str |
Associação Brasileira de Polímeros (ABPol) |
instacron_str |
ABPO |
institution |
ABPO |
reponame_str |
Polímeros (São Carlos. Online) |
collection |
Polímeros (São Carlos. Online) |
repository.name.fl_str_mv |
Polímeros (São Carlos. Online) - Associação Brasileira de Polímeros (ABPol) |
repository.mail.fl_str_mv |
||revista@abpol.org.br |
_version_ |
1754212590059257856 |