Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392014000600022&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/130297 |
Resumo: | Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to stimulate bone regeneration by a simple and low-cost method of surface biofunctionalization of the poly (L-co-D, L lactic acid)-PLDLA scaffolds. The method was obtained by grafting reaction of carboxyl groups (-COOH) on their surface via acrylic acid (AAc) polymerization process, followed by immobilization of collagen type I (Col). Such approach resulted in a surface morphology markedly modified after treatment, with increase of pores and roughness on PLDLA-AAc surfaces and a network of fibrillar collagen deposition in nonspecific areas of PLDLA-Col surfaces. The cytocompatibility of collagen-immobilized scaffolds was significantly improved in terms of cellular adhesion, proliferation, collagen synthesis and maintenance of osteoblast-like phenotype, indicating, therefore, the fundamental role of collagen protein over the biological interactions that occur by bio-recognition mimetic mechanisms at biomaterials interface. These results indicate that the surface modification method used here may be useful as a strategy to develop biofunctional scaffolds, which provide a more successful clinical application of biomaterials in the tissue engineering field. |
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Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffoldsBioabsorbable polymersCollagen immobilizationPLDLA scaffoldsSurface modificationTissue engineeringSurface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to stimulate bone regeneration by a simple and low-cost method of surface biofunctionalization of the poly (L-co-D, L lactic acid)-PLDLA scaffolds. The method was obtained by grafting reaction of carboxyl groups (-COOH) on their surface via acrylic acid (AAc) polymerization process, followed by immobilization of collagen type I (Col). Such approach resulted in a surface morphology markedly modified after treatment, with increase of pores and roughness on PLDLA-AAc surfaces and a network of fibrillar collagen deposition in nonspecific areas of PLDLA-Col surfaces. The cytocompatibility of collagen-immobilized scaffolds was significantly improved in terms of cellular adhesion, proliferation, collagen synthesis and maintenance of osteoblast-like phenotype, indicating, therefore, the fundamental role of collagen protein over the biological interactions that occur by bio-recognition mimetic mechanisms at biomaterials interface. These results indicate that the surface modification method used here may be useful as a strategy to develop biofunctional scaffolds, which provide a more successful clinical application of biomaterials in the tissue engineering field.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)National Institute of Science and Technology in Biofabrication (INCT-Biofabris)Univ Estadual Campinas UNICAMP, Fac Mech Engn, Dept Mat Engn, BR-13083970 Campinas, SP, BrazilPontificia Univ Catolica Sao Paulo PUC SP, Lab Biomat, BR-18030070 Sorocaba, SP, BrazilUniv Estadual Paulista UNESP, Lab Technol Plasmas, BR-18085180 Sorocaba, SP, BrazilUniv Fed Sao Carlos UFSCar, Dept Mat Engn, BR-18052780 Sorocaba, SP, BrazilUniv Fed Sao Carlos UFSCar, Dept Mat Engn, BR-13565905 Sao Carlos, SP, BrazilUniv Estadual Paulista UNESP, Lab Technol Plasmas, BR-18085180 Sorocaba, SP, BrazilFAPESP: 2012/12081-2FAPESP: 2007/05089-9Univ Fed Sao Carlos, Dept Engenharia MaterialsUniversidade Estadual de Campinas (UNICAMP)Pontificia Univ Catolica Sao Paulo PUC SPUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Más, Bruna AntunesCattani, Silvia Mara de MelloRangel, Rita de Cássia Cipriano [UNESP]Ribeiro, Gabrielle de AlmeidaCruz, Nilson Cristino [UNESP]Leite, Fabio de LimaNascente, Pedro Augusto de PaulaDuek, Eliana Aparecida de Rezende2015-11-03T18:06:36Z2015-11-03T18:06:36Z2014-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1523-1534application/pdfhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392014000600022&lng=en&nrm=iso&tlng=enMaterials Research-ibero-american Journal Of Materials. Sao Carlos: Univ Fed Sao Carlos, Dept Engenharia Materials, v. 17, n. 6, p. 1523-1534, 2014.1516-1439http://hdl.handle.net/11449/13029710.1590/1516-1439.269414S1516-14392014000600022WOS:000349766900021S1516-14392014000600022.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Research-ibero-american Journal Of Materials1.1030,398info:eu-repo/semantics/openAccess2023-10-19T06:05:53Zoai:repositorio.unesp.br:11449/130297Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:20:19.375872Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
title |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
spellingShingle |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds Más, Bruna Antunes Bioabsorbable polymers Collagen immobilization PLDLA scaffolds Surface modification Tissue engineering |
title_short |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
title_full |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
title_fullStr |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
title_full_unstemmed |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
title_sort |
Surface characterization and osteoblast-like cells culture on collagen modified PLDLA scaffolds |
author |
Más, Bruna Antunes |
author_facet |
Más, Bruna Antunes Cattani, Silvia Mara de Mello Rangel, Rita de Cássia Cipriano [UNESP] Ribeiro, Gabrielle de Almeida Cruz, Nilson Cristino [UNESP] Leite, Fabio de Lima Nascente, Pedro Augusto de Paula Duek, Eliana Aparecida de Rezende |
author_role |
author |
author2 |
Cattani, Silvia Mara de Mello Rangel, Rita de Cássia Cipriano [UNESP] Ribeiro, Gabrielle de Almeida Cruz, Nilson Cristino [UNESP] Leite, Fabio de Lima Nascente, Pedro Augusto de Paula Duek, Eliana Aparecida de Rezende |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Pontificia Univ Catolica Sao Paulo PUC SP Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Más, Bruna Antunes Cattani, Silvia Mara de Mello Rangel, Rita de Cássia Cipriano [UNESP] Ribeiro, Gabrielle de Almeida Cruz, Nilson Cristino [UNESP] Leite, Fabio de Lima Nascente, Pedro Augusto de Paula Duek, Eliana Aparecida de Rezende |
dc.subject.por.fl_str_mv |
Bioabsorbable polymers Collagen immobilization PLDLA scaffolds Surface modification Tissue engineering |
topic |
Bioabsorbable polymers Collagen immobilization PLDLA scaffolds Surface modification Tissue engineering |
description |
Surface modification techniques based on the grafting of chemical functional groups and immobilization of bioactive molecules have been used to improve biocompatibility and clinical performance of bioabsorbable scaffolds in tissue engineering and medicine regenerative applications. This study aimed at developing and characterizing a biomimetic surface to stimulate bone regeneration by a simple and low-cost method of surface biofunctionalization of the poly (L-co-D, L lactic acid)-PLDLA scaffolds. The method was obtained by grafting reaction of carboxyl groups (-COOH) on their surface via acrylic acid (AAc) polymerization process, followed by immobilization of collagen type I (Col). Such approach resulted in a surface morphology markedly modified after treatment, with increase of pores and roughness on PLDLA-AAc surfaces and a network of fibrillar collagen deposition in nonspecific areas of PLDLA-Col surfaces. The cytocompatibility of collagen-immobilized scaffolds was significantly improved in terms of cellular adhesion, proliferation, collagen synthesis and maintenance of osteoblast-like phenotype, indicating, therefore, the fundamental role of collagen protein over the biological interactions that occur by bio-recognition mimetic mechanisms at biomaterials interface. These results indicate that the surface modification method used here may be useful as a strategy to develop biofunctional scaffolds, which provide a more successful clinical application of biomaterials in the tissue engineering field. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-11-01 2015-11-03T18:06:36Z 2015-11-03T18:06:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392014000600022&lng=en&nrm=iso&tlng=en Materials Research-ibero-american Journal Of Materials. Sao Carlos: Univ Fed Sao Carlos, Dept Engenharia Materials, v. 17, n. 6, p. 1523-1534, 2014. 1516-1439 http://hdl.handle.net/11449/130297 10.1590/1516-1439.269414 S1516-14392014000600022 WOS:000349766900021 S1516-14392014000600022.pdf |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-14392014000600022&lng=en&nrm=iso&tlng=en http://hdl.handle.net/11449/130297 |
identifier_str_mv |
Materials Research-ibero-american Journal Of Materials. Sao Carlos: Univ Fed Sao Carlos, Dept Engenharia Materials, v. 17, n. 6, p. 1523-1534, 2014. 1516-1439 10.1590/1516-1439.269414 S1516-14392014000600022 WOS:000349766900021 S1516-14392014000600022.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Research-ibero-american Journal Of Materials 1.103 0,398 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1523-1534 application/pdf |
dc.publisher.none.fl_str_mv |
Univ Fed Sao Carlos, Dept Engenharia Materials |
publisher.none.fl_str_mv |
Univ Fed Sao Carlos, Dept Engenharia Materials |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128497631100928 |