PRP Plasma Gel protocolo de obtención para uso en procedimientos estéticos

• Autor(a) principal: Rossi Junior, Renato
• Data de Publicação: 2020
• Outros Autores: Leal Rossi, Camila, Leal Rossi, Adriana, Rossi de Souza, Bianca
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Aesthetic Orofacial Science
• Texto Completo: https://ahof.emnuvens.com.br/ahof/article/view/9
• topic: plasma rico em plaquetas; preenchedores dérmicos; estética; plasma rico em plaquetas; rellenos dérmicos; estética; platelet rich plasma; dermal fillers; esthetics

The objective of this article was to present a protocol for obtaining platelet derivatives that allows its use in facial aesthetic procedures. Methods: Platelet Rich Plasma (PRP) was obtained from blood collection in 6 tubes of 10 ml BD Vacutainer® 0.109 mol / L Buffered Sodium Citrate that were centrifuged in a 150G for 15 minutes. Then, the yellow portion of each tube was collected, and the content obtained divided into two tubes (Vacuette® tube, 9 ml, Greiner Bio-One International, without additives) which were centrifuged again at 300 g for 5 minutes. A plasma portion of 60% of the tube volume was aspirated into a 3 ml syringe and placed in a syringe warmer (APAG, Silfradent®) at 75ºC for 15 minutes to obtain a plasma gel. The remaining portion was gently mixed to resuspend the platelets in the remaining plasma thus forming a concentrated Platelet Rich Plasma. The PRP Plasma Gel was then obtained by mixing the heated Plasma Gel with the PRP concentrate in a 1:1 ratio. 2:1 and 3:1 to achieve different gel densities suitable for aesthetic purposes. Discussion: The PRP Plasma Gel produced using the 5G protocol is a practical alternative to the use of commercial fillers in some facial areas. The growth factors of the platelet concentrate, its angiogenic factors and fibrin promote tissue rejuvenation, repairing the fibers and cells lost by aging. Conclusion: The use of the PRP Plasma Gel obtained by the proposed protocol is a simple, safe and accessible procedure for a greater number of patients, promoting a progressive improvement of the skin.