Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality

Detalhes bibliográficos
Autor(a) principal: Cruz,Sara Michelly
Data de Publicação: 2013
Outros Autores: Nery,Marcela Carlota, Rocha,Adriana de Souza, Von Pinho,Édila Vilela de Resende, Andrade,Paulo César de Resende, Dias,Denise Cunha Fernandes dos Santos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Journal of Seed Science
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010
Resumo: The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.
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spelling Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed qualityaccelerated agingelectrical conductivityenzymatic profileThe aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.ABRATES - Associação Brasileira de Tecnologia de Sementes2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010Journal of Seed Science v.35 n.4 2013reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/S2317-15372013000400010info:eu-repo/semantics/openAccessCruz,Sara MichellyNery,Marcela CarlotaRocha,Adriana de SouzaVon Pinho,Édila Vilela de ResendeAndrade,Paulo César de ResendeDias,Denise Cunha Fernandes dos Santoseng2014-01-20T00:00:00Zoai:scielo:S2317-15372013000400010Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2014-01-20T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false
dc.title.none.fl_str_mv Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
spellingShingle Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
Cruz,Sara Michelly
accelerated aging
electrical conductivity
enzymatic profile
title_short Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_full Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_fullStr Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_full_unstemmed Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_sort Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
author Cruz,Sara Michelly
author_facet Cruz,Sara Michelly
Nery,Marcela Carlota
Rocha,Adriana de Souza
Von Pinho,Édila Vilela de Resende
Andrade,Paulo César de Resende
Dias,Denise Cunha Fernandes dos Santos
author_role author
author2 Nery,Marcela Carlota
Rocha,Adriana de Souza
Von Pinho,Édila Vilela de Resende
Andrade,Paulo César de Resende
Dias,Denise Cunha Fernandes dos Santos
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cruz,Sara Michelly
Nery,Marcela Carlota
Rocha,Adriana de Souza
Von Pinho,Édila Vilela de Resende
Andrade,Paulo César de Resende
Dias,Denise Cunha Fernandes dos Santos
dc.subject.por.fl_str_mv accelerated aging
electrical conductivity
enzymatic profile
topic accelerated aging
electrical conductivity
enzymatic profile
description The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S2317-15372013000400010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
publisher.none.fl_str_mv ABRATES - Associação Brasileira de Tecnologia de Sementes
dc.source.none.fl_str_mv Journal of Seed Science v.35 n.4 2013
reponame:Journal of Seed Science
instname:Associação Brasileira de Tecnologia de Sementes (ABRATES)
instacron:ABRATES
instname_str Associação Brasileira de Tecnologia de Sementes (ABRATES)
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institution ABRATES
reponame_str Journal of Seed Science
collection Journal of Seed Science
repository.name.fl_str_mv Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)
repository.mail.fl_str_mv ||abrates@abrates.org.br
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