Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010 |
Resumo: | The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar. |
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Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed qualityaccelerated agingelectrical conductivityenzymatic profileThe aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.ABRATES - Associação Brasileira de Tecnologia de Sementes2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010Journal of Seed Science v.35 n.4 2013reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/S2317-15372013000400010info:eu-repo/semantics/openAccessCruz,Sara MichellyNery,Marcela CarlotaRocha,Adriana de SouzaVon Pinho,Édila Vilela de ResendeAndrade,Paulo César de ResendeDias,Denise Cunha Fernandes dos Santoseng2014-01-20T00:00:00Zoai:scielo:S2317-15372013000400010Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2014-01-20T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
spellingShingle |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality Cruz,Sara Michelly accelerated aging electrical conductivity enzymatic profile |
title_short |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_full |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_fullStr |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_full_unstemmed |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_sort |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
author |
Cruz,Sara Michelly |
author_facet |
Cruz,Sara Michelly Nery,Marcela Carlota Rocha,Adriana de Souza Von Pinho,Édila Vilela de Resende Andrade,Paulo César de Resende Dias,Denise Cunha Fernandes dos Santos |
author_role |
author |
author2 |
Nery,Marcela Carlota Rocha,Adriana de Souza Von Pinho,Édila Vilela de Resende Andrade,Paulo César de Resende Dias,Denise Cunha Fernandes dos Santos |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cruz,Sara Michelly Nery,Marcela Carlota Rocha,Adriana de Souza Von Pinho,Édila Vilela de Resende Andrade,Paulo César de Resende Dias,Denise Cunha Fernandes dos Santos |
dc.subject.por.fl_str_mv |
accelerated aging electrical conductivity enzymatic profile |
topic |
accelerated aging electrical conductivity enzymatic profile |
description |
The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372013000400010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S2317-15372013000400010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.35 n.4 2013 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
_version_ |
1754212981665693696 |