Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality

Detalhes bibliográficos
Autor(a) principal: Dias, Denise Cunha Fernandes dos Santos
Data de Publicação: 2013
Outros Autores: Cruz, Sara Michelly, Nery, Marcela Carlota, Rocha, Adriana de Souza, Pinho, Édila Vilela de Resende Von, Andrade, Paulo César de Resende
Tipo de documento: Artigo
Idioma: eng
Título da fonte: LOCUS Repositório Institucional da UFV
Texto Completo: http://dx.doi.org/10.1590/S2317-15372013000400010
http://locus.ufv.br//handle/123456789/25976
Resumo: The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.
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spelling Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed qualityAccelerated agingElectrical conductivityEnzymatic profileEnvelhecimento aceleradoCondutividade elétricaPerfil enzimáticoThe aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.O objetivo deste estudo foi adequar a metodologia dos testes de envelhecimento acelerado (EA) e condutividade elétrica (CE) para avaliar a qualidade de sementes de crambe, bem como verificar a atividade enzimática nessa semente em relação às diferenças de vigor. Para isso sementes de crambe (cv FMS Brilhante) foram submetidas ao teste EA, durante 0, 24, 48, 72 e 96 h, a 42 ºC, com e sem solução saturada de NaCl; e ao teste CE, com imersão das sementes em água deionizada, nas proporções de 25/25, 25/50, 50/50 e 50/75 (semente/água), durante 2, 4, 6, 8, 10, 12, 14, 16 e 18 h. O perfil eletroforético para esterase, superóxido dismutase, catalase, isocitrato liase, álcool desidrogenase e malato desidrogenase foi avaliado em gel de poliacrilamida (7,5%). O teste EA conduzido a 42 ºC, durante 96 h, foi eficiente para detectar diferenças nos níveis de qualidade; porém, o teste CE não foi adequado para avaliar o vigor dessas sementes. As bandas coradas para esterase, superóxido dismutase e catalase foram mais nítidas para sementes menos vigorosas; e as bandas para isocitrato liase e álcool desidrogenase foram menos nítidas para sementes menos vigorosas. Para a enzima malato desidrogenase a intensidade das bandas foi semelhante.Journal of Seed Science2019-06-28T13:47:40Z2019-06-28T13:47:40Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf2317-1545http://dx.doi.org/10.1590/S2317-15372013000400010http://locus.ufv.br//handle/123456789/25976engv. 35, n. 04, p. 485- 494, 2013Dias, Denise Cunha Fernandes dos SantosCruz, Sara MichellyNery, Marcela CarlotaRocha, Adriana de SouzaPinho, Édila Vilela de Resende VonAndrade, Paulo César de Resendeinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:30:51Zoai:locus.ufv.br:123456789/25976Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:30:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false
dc.title.none.fl_str_mv Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
spellingShingle Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
Dias, Denise Cunha Fernandes dos Santos
Accelerated aging
Electrical conductivity
Enzymatic profile
Envelhecimento acelerado
Condutividade elétrica
Perfil enzimático
title_short Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_full Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_fullStr Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_full_unstemmed Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
title_sort Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
author Dias, Denise Cunha Fernandes dos Santos
author_facet Dias, Denise Cunha Fernandes dos Santos
Cruz, Sara Michelly
Nery, Marcela Carlota
Rocha, Adriana de Souza
Pinho, Édila Vilela de Resende Von
Andrade, Paulo César de Resende
author_role author
author2 Cruz, Sara Michelly
Nery, Marcela Carlota
Rocha, Adriana de Souza
Pinho, Édila Vilela de Resende Von
Andrade, Paulo César de Resende
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Dias, Denise Cunha Fernandes dos Santos
Cruz, Sara Michelly
Nery, Marcela Carlota
Rocha, Adriana de Souza
Pinho, Édila Vilela de Resende Von
Andrade, Paulo César de Resende
dc.subject.por.fl_str_mv Accelerated aging
Electrical conductivity
Enzymatic profile
Envelhecimento acelerado
Condutividade elétrica
Perfil enzimático
topic Accelerated aging
Electrical conductivity
Enzymatic profile
Envelhecimento acelerado
Condutividade elétrica
Perfil enzimático
description The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.
publishDate 2013
dc.date.none.fl_str_mv 2013
2019-06-28T13:47:40Z
2019-06-28T13:47:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv 2317-1545
http://dx.doi.org/10.1590/S2317-15372013000400010
http://locus.ufv.br//handle/123456789/25976
identifier_str_mv 2317-1545
url http://dx.doi.org/10.1590/S2317-15372013000400010
http://locus.ufv.br//handle/123456789/25976
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv v. 35, n. 04, p. 485- 494, 2013
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Seed Science
publisher.none.fl_str_mv Journal of Seed Science
dc.source.none.fl_str_mv reponame:LOCUS Repositório Institucional da UFV
instname:Universidade Federal de Viçosa (UFV)
instacron:UFV
instname_str Universidade Federal de Viçosa (UFV)
instacron_str UFV
institution UFV
reponame_str LOCUS Repositório Institucional da UFV
collection LOCUS Repositório Institucional da UFV
repository.name.fl_str_mv LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)
repository.mail.fl_str_mv fabiojreis@ufv.br
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