Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | LOCUS Repositório Institucional da UFV |
Texto Completo: | http://dx.doi.org/10.1590/S2317-15372013000400010 http://locus.ufv.br//handle/123456789/25976 |
Resumo: | The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar. |
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Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed qualityAccelerated agingElectrical conductivityEnzymatic profileEnvelhecimento aceleradoCondutividade elétricaPerfil enzimáticoThe aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar.O objetivo deste estudo foi adequar a metodologia dos testes de envelhecimento acelerado (EA) e condutividade elétrica (CE) para avaliar a qualidade de sementes de crambe, bem como verificar a atividade enzimática nessa semente em relação às diferenças de vigor. Para isso sementes de crambe (cv FMS Brilhante) foram submetidas ao teste EA, durante 0, 24, 48, 72 e 96 h, a 42 ºC, com e sem solução saturada de NaCl; e ao teste CE, com imersão das sementes em água deionizada, nas proporções de 25/25, 25/50, 50/50 e 50/75 (semente/água), durante 2, 4, 6, 8, 10, 12, 14, 16 e 18 h. O perfil eletroforético para esterase, superóxido dismutase, catalase, isocitrato liase, álcool desidrogenase e malato desidrogenase foi avaliado em gel de poliacrilamida (7,5%). O teste EA conduzido a 42 ºC, durante 96 h, foi eficiente para detectar diferenças nos níveis de qualidade; porém, o teste CE não foi adequado para avaliar o vigor dessas sementes. As bandas coradas para esterase, superóxido dismutase e catalase foram mais nítidas para sementes menos vigorosas; e as bandas para isocitrato liase e álcool desidrogenase foram menos nítidas para sementes menos vigorosas. Para a enzima malato desidrogenase a intensidade das bandas foi semelhante.Journal of Seed Science2019-06-28T13:47:40Z2019-06-28T13:47:40Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlepdfapplication/pdf2317-1545http://dx.doi.org/10.1590/S2317-15372013000400010http://locus.ufv.br//handle/123456789/25976engv. 35, n. 04, p. 485- 494, 2013Dias, Denise Cunha Fernandes dos SantosCruz, Sara MichellyNery, Marcela CarlotaRocha, Adriana de SouzaPinho, Édila Vilela de Resende VonAndrade, Paulo César de Resendeinfo:eu-repo/semantics/openAccessreponame:LOCUS Repositório Institucional da UFVinstname:Universidade Federal de Viçosa (UFV)instacron:UFV2024-07-12T06:30:51Zoai:locus.ufv.br:123456789/25976Repositório InstitucionalPUBhttps://www.locus.ufv.br/oai/requestfabiojreis@ufv.bropendoar:21452024-07-12T06:30:51LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV)false |
dc.title.none.fl_str_mv |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
spellingShingle |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality Dias, Denise Cunha Fernandes dos Santos Accelerated aging Electrical conductivity Enzymatic profile Envelhecimento acelerado Condutividade elétrica Perfil enzimático |
title_short |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_full |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_fullStr |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_full_unstemmed |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
title_sort |
Vigor tests for evaluation of crambe (Crambe abyssinica Hochst) seed quality |
author |
Dias, Denise Cunha Fernandes dos Santos |
author_facet |
Dias, Denise Cunha Fernandes dos Santos Cruz, Sara Michelly Nery, Marcela Carlota Rocha, Adriana de Souza Pinho, Édila Vilela de Resende Von Andrade, Paulo César de Resende |
author_role |
author |
author2 |
Cruz, Sara Michelly Nery, Marcela Carlota Rocha, Adriana de Souza Pinho, Édila Vilela de Resende Von Andrade, Paulo César de Resende |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dias, Denise Cunha Fernandes dos Santos Cruz, Sara Michelly Nery, Marcela Carlota Rocha, Adriana de Souza Pinho, Édila Vilela de Resende Von Andrade, Paulo César de Resende |
dc.subject.por.fl_str_mv |
Accelerated aging Electrical conductivity Enzymatic profile Envelhecimento acelerado Condutividade elétrica Perfil enzimático |
topic |
Accelerated aging Electrical conductivity Enzymatic profile Envelhecimento acelerado Condutividade elétrica Perfil enzimático |
description |
The aim of this study was adapting the methodology of the accelerated aging test (AA) and electrical conductivity test (EC) to evaluate seed quality of crambe, as well as verifying enzymatic activity in relation to vigor differences. Crambe seed lots, cv. FMS Brilhante, were evaluated by the AA test, for periods of 0, 24, 48, 72 and 96 h, at 42 °C, with and without NaCl saturated solution; and the EC test, by soaking seeds into deionized water (mL) in the ratios of 25/25, 25/50, 50/50 and 50/75 (seed/water) for 2, 4, 6, 8, 10, 12, 14, 16 and 18 h. The electrophoretic profile for isozymes esterase, superoxide dismutase, catalase, isocitrate lyase, alcohol dehydrogenase and malate dehydrogenase was evaluated on polyacrylamide gel (7.5%). The AA test conducted at 42 °C, for 96 h, was efficient to detect differences on seed quality levels; however, the EC test was not suitable to evaluate vigor of those seeds. The bands stained for isozymes esterase, superoxide dismutase and catalase were evident for the more vigorous seeds; and the bands for isozymes isocitrate lyase and alcohol dehydrogenase were less evident for the less vigorous seeds. For enzyme malate dehydrogenase enzyme intensity of bands was similar. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2019-06-28T13:47:40Z 2019-06-28T13:47:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
2317-1545 http://dx.doi.org/10.1590/S2317-15372013000400010 http://locus.ufv.br//handle/123456789/25976 |
identifier_str_mv |
2317-1545 |
url |
http://dx.doi.org/10.1590/S2317-15372013000400010 http://locus.ufv.br//handle/123456789/25976 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
v. 35, n. 04, p. 485- 494, 2013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
pdf application/pdf |
dc.publisher.none.fl_str_mv |
Journal of Seed Science |
publisher.none.fl_str_mv |
Journal of Seed Science |
dc.source.none.fl_str_mv |
reponame:LOCUS Repositório Institucional da UFV instname:Universidade Federal de Viçosa (UFV) instacron:UFV |
instname_str |
Universidade Federal de Viçosa (UFV) |
instacron_str |
UFV |
institution |
UFV |
reponame_str |
LOCUS Repositório Institucional da UFV |
collection |
LOCUS Repositório Institucional da UFV |
repository.name.fl_str_mv |
LOCUS Repositório Institucional da UFV - Universidade Federal de Viçosa (UFV) |
repository.mail.fl_str_mv |
fabiojreis@ufv.br |
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1817559843563634688 |