Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Journal of Seed Science |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372015000200102 |
Resumo: | Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality. |
id |
ABRATES-1_8a5ab2402a771e9bc4740e611972e53b |
---|---|
oai_identifier_str |
oai:scielo:S2317-15372015000200102 |
network_acronym_str |
ABRATES-1 |
network_name_str |
Journal of Seed Science |
repository_id_str |
|
spelling |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to dryingisoenzymesenzymesqRT-PCRHandroanthus serratifoliusHandroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality.ABRATES - Associação Brasileira de Tecnologia de Sementes2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372015000200102Journal of Seed Science v.37 n.2 2015reponame:Journal of Seed Scienceinstname:Associação Brasileira de Tecnologia de Sementes (ABRATES)instacron:ABRATES10.1590/2317-1545v37n2144303info:eu-repo/semantics/openAccessGonçalves,Leandra Helena do NascimentoSantos,Heloísa Oliveira dosVon Pinho,Édila Vilela de ResendeAndrade,Thaís deVon Pinho,Iolanda VilelaPereira,Rucyan Walaceeng2015-07-01T00:00:00Zoai:scielo:S2317-15372015000200102Revistahttp://www.scielo.br/scielo.php?script=sci_serial&pid=2317-1537&lng=en&nrm=isohttps://old.scielo.br/oai/scielo-oai.php||abrates@abrates.org.br2317-15452317-1537opendoar:2015-07-01T00:00Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES)false |
dc.title.none.fl_str_mv |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
title |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
spellingShingle |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying Gonçalves,Leandra Helena do Nascimento isoenzymes enzymes qRT-PCR Handroanthus serratifolius |
title_short |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
title_full |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
title_fullStr |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
title_full_unstemmed |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
title_sort |
Physiological quality and expression of genes in seeds of Handroanthus serratifolius subjected to drying |
author |
Gonçalves,Leandra Helena do Nascimento |
author_facet |
Gonçalves,Leandra Helena do Nascimento Santos,Heloísa Oliveira dos Von Pinho,Édila Vilela de Resende Andrade,Thaís de Von Pinho,Iolanda Vilela Pereira,Rucyan Walace |
author_role |
author |
author2 |
Santos,Heloísa Oliveira dos Von Pinho,Édila Vilela de Resende Andrade,Thaís de Von Pinho,Iolanda Vilela Pereira,Rucyan Walace |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gonçalves,Leandra Helena do Nascimento Santos,Heloísa Oliveira dos Von Pinho,Édila Vilela de Resende Andrade,Thaís de Von Pinho,Iolanda Vilela Pereira,Rucyan Walace |
dc.subject.por.fl_str_mv |
isoenzymes enzymes qRT-PCR Handroanthus serratifolius |
topic |
isoenzymes enzymes qRT-PCR Handroanthus serratifolius |
description |
Handroanthus serratifolius seeds are considered orthodox and have some mechanisms that assist in desiccation tolerance, like heat-resistant proteins and the presence of protective systems against free radical production. In order to investigate the drying effect on the quality of Handroanthus serratifolius seeds collected with 28% water content two methods were used: slow drying (30 °C) and fast drying (45 °C) until water contents equaled 20%, 15%, 10% and 5%. Seed quality was assessed by germination test, first count of germination, speed germination, emergence and speed emergence. The expression of heat-resistant proteins and enzymes catalase, esterase and isocitrate lyase was assessed by electrophoresis. The gene expression of sHSP17.5, CAT3 and ICL6 was quantified by real time PCR. Higher values of germination and vigor were obtained in seeds with 20% water content, subjected to fast drying. There was induction of heat-resistant proteins expression for both drying methods. There was variation in the electrophoretic pattern of catalase, esterase and isocitrate lyase in seeds subjected to different treatments. The gene expression in Handroanthus serratifolius seeds varies depending on methods of drying and water content of seeds. The drying of Handroanthus serratifolius seeds until the water content between 15 and 10% compromises their physiological quality. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372015000200102 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2317-15372015000200102 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2317-1545v37n2144303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
publisher.none.fl_str_mv |
ABRATES - Associação Brasileira de Tecnologia de Sementes |
dc.source.none.fl_str_mv |
Journal of Seed Science v.37 n.2 2015 reponame:Journal of Seed Science instname:Associação Brasileira de Tecnologia de Sementes (ABRATES) instacron:ABRATES |
instname_str |
Associação Brasileira de Tecnologia de Sementes (ABRATES) |
instacron_str |
ABRATES |
institution |
ABRATES |
reponame_str |
Journal of Seed Science |
collection |
Journal of Seed Science |
repository.name.fl_str_mv |
Journal of Seed Science - Associação Brasileira de Tecnologia de Sementes (ABRATES) |
repository.mail.fl_str_mv |
||abrates@abrates.org.br |
_version_ |
1754212982042132480 |