Ki-67 expression in mature B-cell neoplasms: a flow cytometry study

Detalhes bibliográficos
Autor(a) principal: Marcondes,Natália
Data de Publicação: 2018
Outros Autores: Fernandes,Flavo, Faulhaber,Gustavo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302018000600525
Resumo: SUMMARY OBJECTIVE: Ki-67 is a nuclear protein associated with cellular proliferation in normal or leukemic conditions that can help identify more aggressive diseases and is usually evaluated with immunohistochemistry. The aim of this was to assess Ki-67 expression on mature B-cell neoplasms samples with flow cytometry immunophenotyping. METHOD: After surface staining with CD19 and CD45, intracellular staining for Ki-67 was performed in leukemic mature B-cells. Ki-67 expression was evaluated with flow cytometry. RESULTS: Ki-67 expression was higher in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma cases. It was also associated with CD38 mean fluorescence intensity. CONCLUSIONS: Ki-67 expression evaluated by flow cytometry can be a useful tool in the diagnosis of mature B-cell neoplasms. More studies are needed to validate Ki-67 assessment with flow cytometry immunophenotyping.
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spelling Ki-67 expression in mature B-cell neoplasms: a flow cytometry studyFlow cytometryImmunophenotypingKi-67 AntigenSUMMARY OBJECTIVE: Ki-67 is a nuclear protein associated with cellular proliferation in normal or leukemic conditions that can help identify more aggressive diseases and is usually evaluated with immunohistochemistry. The aim of this was to assess Ki-67 expression on mature B-cell neoplasms samples with flow cytometry immunophenotyping. METHOD: After surface staining with CD19 and CD45, intracellular staining for Ki-67 was performed in leukemic mature B-cells. Ki-67 expression was evaluated with flow cytometry. RESULTS: Ki-67 expression was higher in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma cases. It was also associated with CD38 mean fluorescence intensity. CONCLUSIONS: Ki-67 expression evaluated by flow cytometry can be a useful tool in the diagnosis of mature B-cell neoplasms. More studies are needed to validate Ki-67 assessment with flow cytometry immunophenotyping.Associação Médica Brasileira2018-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302018000600525Revista da Associação Médica Brasileira v.64 n.6 2018reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.64.06.525info:eu-repo/semantics/openAccessMarcondes,NatáliaFernandes,FlavoFaulhaber,Gustavoeng2018-09-19T00:00:00Zoai:scielo:S0104-42302018000600525Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2018-09-19T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
title Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
spellingShingle Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
Marcondes,Natália
Flow cytometry
Immunophenotyping
Ki-67 Antigen
title_short Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
title_full Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
title_fullStr Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
title_full_unstemmed Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
title_sort Ki-67 expression in mature B-cell neoplasms: a flow cytometry study
author Marcondes,Natália
author_facet Marcondes,Natália
Fernandes,Flavo
Faulhaber,Gustavo
author_role author
author2 Fernandes,Flavo
Faulhaber,Gustavo
author2_role author
author
dc.contributor.author.fl_str_mv Marcondes,Natália
Fernandes,Flavo
Faulhaber,Gustavo
dc.subject.por.fl_str_mv Flow cytometry
Immunophenotyping
Ki-67 Antigen
topic Flow cytometry
Immunophenotyping
Ki-67 Antigen
description SUMMARY OBJECTIVE: Ki-67 is a nuclear protein associated with cellular proliferation in normal or leukemic conditions that can help identify more aggressive diseases and is usually evaluated with immunohistochemistry. The aim of this was to assess Ki-67 expression on mature B-cell neoplasms samples with flow cytometry immunophenotyping. METHOD: After surface staining with CD19 and CD45, intracellular staining for Ki-67 was performed in leukemic mature B-cells. Ki-67 expression was evaluated with flow cytometry. RESULTS: Ki-67 expression was higher in mantle cell lymphoma, Burkitt lymphoma, and diffuse large B-cell lymphoma cases. It was also associated with CD38 mean fluorescence intensity. CONCLUSIONS: Ki-67 expression evaluated by flow cytometry can be a useful tool in the diagnosis of mature B-cell neoplasms. More studies are needed to validate Ki-67 assessment with flow cytometry immunophenotyping.
publishDate 2018
dc.date.none.fl_str_mv 2018-06-01
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dc.relation.none.fl_str_mv 10.1590/1806-9282.64.06.525
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dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.64 n.6 2018
reponame:Revista da Associação Médica Brasileira (Online)
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