COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway

Detalhes bibliográficos
Autor(a) principal: Guo,Hong-Li
Data de Publicação: 2020
Outros Autores: Chen,Gang, Song,Ze-Long, Sun,Jia, Gao,Xi-Hai, Han,Yu-Xia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000600740
Resumo: SUMMARY OBJECTIVE In this study, we aimed to investigate the role of COL6A3 on cell motility and the PI3K/AKT signaling pathway in osteosarcoma. METHODS The relative expression of COL6A3 was achieved from a GEO dataset in osteosarcoma tissue. siRNA technology was applied to decrease the COL6A3 expression in cells, and cell counting kit-8 (CCK-8) assay and colony formation analysis were used to examine the cell proliferation potential. Knockdown COL6A3 made the proliferation and colony formation abilities worse than the COL6A3 without interference. Likewise, in contrast to the si-con group, cell invasion and migration were inhibited in the si-COL6A3 group. Moreover, the western blot results suggested that the PI3K/AKT signaling pathway was manipulated by measuring the protein expression of the PI3K/AKT pathway-related markers, due to the COL6A3 inhibition. CONCLUSION COL6A3 plays a crucial role in modulating various aspects of the progression of osteosarcoma, which would provide a potentially effective treatment for osteosarcoma.
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spelling COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathwayOsteosarcomaNeoplasms, bone tissueMolecular targeted therapyCollagen type VISUMMARY OBJECTIVE In this study, we aimed to investigate the role of COL6A3 on cell motility and the PI3K/AKT signaling pathway in osteosarcoma. METHODS The relative expression of COL6A3 was achieved from a GEO dataset in osteosarcoma tissue. siRNA technology was applied to decrease the COL6A3 expression in cells, and cell counting kit-8 (CCK-8) assay and colony formation analysis were used to examine the cell proliferation potential. Knockdown COL6A3 made the proliferation and colony formation abilities worse than the COL6A3 without interference. Likewise, in contrast to the si-con group, cell invasion and migration were inhibited in the si-COL6A3 group. Moreover, the western blot results suggested that the PI3K/AKT signaling pathway was manipulated by measuring the protein expression of the PI3K/AKT pathway-related markers, due to the COL6A3 inhibition. CONCLUSION COL6A3 plays a crucial role in modulating various aspects of the progression of osteosarcoma, which would provide a potentially effective treatment for osteosarcoma.Associação Médica Brasileira2020-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000600740Revista da Associação Médica Brasileira v.66 n.6 2020reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.66.6.740info:eu-repo/semantics/openAccessGuo,Hong-LiChen,GangSong,Ze-LongSun,JiaGao,Xi-HaiHan,Yu-Xiaeng2020-07-17T00:00:00Zoai:scielo:S0104-42302020000600740Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2020-07-17T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
title COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
spellingShingle COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
Guo,Hong-Li
Osteosarcoma
Neoplasms, bone tissue
Molecular targeted therapy
Collagen type VI
title_short COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
title_full COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
title_fullStr COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
title_full_unstemmed COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
title_sort COL6A3 promotes cellular malignancy of osteosarcoma by activating the PI3K/AKT pathway
author Guo,Hong-Li
author_facet Guo,Hong-Li
Chen,Gang
Song,Ze-Long
Sun,Jia
Gao,Xi-Hai
Han,Yu-Xia
author_role author
author2 Chen,Gang
Song,Ze-Long
Sun,Jia
Gao,Xi-Hai
Han,Yu-Xia
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Guo,Hong-Li
Chen,Gang
Song,Ze-Long
Sun,Jia
Gao,Xi-Hai
Han,Yu-Xia
dc.subject.por.fl_str_mv Osteosarcoma
Neoplasms, bone tissue
Molecular targeted therapy
Collagen type VI
topic Osteosarcoma
Neoplasms, bone tissue
Molecular targeted therapy
Collagen type VI
description SUMMARY OBJECTIVE In this study, we aimed to investigate the role of COL6A3 on cell motility and the PI3K/AKT signaling pathway in osteosarcoma. METHODS The relative expression of COL6A3 was achieved from a GEO dataset in osteosarcoma tissue. siRNA technology was applied to decrease the COL6A3 expression in cells, and cell counting kit-8 (CCK-8) assay and colony formation analysis were used to examine the cell proliferation potential. Knockdown COL6A3 made the proliferation and colony formation abilities worse than the COL6A3 without interference. Likewise, in contrast to the si-con group, cell invasion and migration were inhibited in the si-COL6A3 group. Moreover, the western blot results suggested that the PI3K/AKT signaling pathway was manipulated by measuring the protein expression of the PI3K/AKT pathway-related markers, due to the COL6A3 inhibition. CONCLUSION COL6A3 plays a crucial role in modulating various aspects of the progression of osteosarcoma, which would provide a potentially effective treatment for osteosarcoma.
publishDate 2020
dc.date.none.fl_str_mv 2020-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000600740
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000600740
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9282.66.6.740
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.66 n.6 2020
reponame:Revista da Associação Médica Brasileira (Online)
instname:Associação Médica Brasileira (AMB)
instacron:AMB
instname_str Associação Médica Brasileira (AMB)
instacron_str AMB
institution AMB
reponame_str Revista da Associação Médica Brasileira (Online)
collection Revista da Associação Médica Brasileira (Online)
repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
repository.mail.fl_str_mv ||ramb@amb.org.br
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