ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer

Detalhes bibliográficos
Autor(a) principal: Shang,Xue-Qin
Data de Publicação: 2020
Outros Autores: Liu,Kui-Liang, Li,Qian, Lao,Yue-Qiong, Li,Nan-Shan, Wu,Jing
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000100042
Resumo: SUMMARY OBJECTIVE ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression. Methods The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness. Results The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings. Conclusion The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.
id AMB-1_c9128dc1c2331fb012623ee698033443
oai_identifier_str oai:scielo:S0104-42302020000100042
network_acronym_str AMB-1
network_name_str Revista da Associação Médica Brasileira (Online)
repository_id_str
spelling ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancerNeoplasmsNeoplasm MetastasisADAMTS4 ProteinSUMMARY OBJECTIVE ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression. Methods The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness. Results The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings. Conclusion The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.Associação Médica Brasileira2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000100042Revista da Associação Médica Brasileira v.66 n.1 2020reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.66.1.42info:eu-repo/semantics/openAccessShang,Xue-QinLiu,Kui-LiangLi,QianLao,Yue-QiongLi,Nan-ShanWu,Jingeng2020-02-19T00:00:00Zoai:scielo:S0104-42302020000100042Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2020-02-19T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
title ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
spellingShingle ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
Shang,Xue-Qin
Neoplasms
Neoplasm Metastasis
ADAMTS4 Protein
title_short ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
title_full ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
title_fullStr ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
title_full_unstemmed ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
title_sort ADAMTS4 is upregulated in colorectal cancer and could be a useful prognostic indicator of colorectal cancer
author Shang,Xue-Qin
author_facet Shang,Xue-Qin
Liu,Kui-Liang
Li,Qian
Lao,Yue-Qiong
Li,Nan-Shan
Wu,Jing
author_role author
author2 Liu,Kui-Liang
Li,Qian
Lao,Yue-Qiong
Li,Nan-Shan
Wu,Jing
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Shang,Xue-Qin
Liu,Kui-Liang
Li,Qian
Lao,Yue-Qiong
Li,Nan-Shan
Wu,Jing
dc.subject.por.fl_str_mv Neoplasms
Neoplasm Metastasis
ADAMTS4 Protein
topic Neoplasms
Neoplasm Metastasis
ADAMTS4 Protein
description SUMMARY OBJECTIVE ADAMTS4 is a member of the ADAMTS4 family, which secretes proteinases. The mechanism of tumor metastasis may be correlated to its promotion of angiogenesis. It was determined whether ADAMTS4 participates in colorectal cancer progression. Methods The expression in clinical samples and CRC cell lines was investigated. Using immunohistochemistry (IHC), fluorescence in situ hybridization (FISH), and RT-PCR, the expression of ADAMTS4 was determined in colorectal tumors of different cancer stages and anatomic sites, and in three cell lines of different aggressiveness. Results The overexpression of ADAMTS4 was observed in tissue samples by IHC, and this was mainly located in the cytoplasm, as detected by FISH. The qRT-PCR and western blot analyses further supported the clinical sample findings. Conclusion The present data support the notion that the overexpression of ADAMTS4 in CRC might be useful as a non-invasive biomarker for detecting CRC in patients.
publishDate 2020
dc.date.none.fl_str_mv 2020-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000100042
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000100042
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1806-9282.66.1.42
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.66 n.1 2020
reponame:Revista da Associação Médica Brasileira (Online)
instname:Associação Médica Brasileira (AMB)
instacron:AMB
instname_str Associação Médica Brasileira (AMB)
instacron_str AMB
institution AMB
reponame_str Revista da Associação Médica Brasileira (Online)
collection Revista da Associação Médica Brasileira (Online)
repository.name.fl_str_mv Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)
repository.mail.fl_str_mv ||ramb@amb.org.br
_version_ 1754212834715107328

Registros relacionados