The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa

Detalhes bibliográficos
Autor(a) principal: Franco,Paulo Celso
Data de Publicação: 2020
Outros Autores: Simões,Ricardo Santos, Carbonel,Adriana Aparecida Ferraz, Sasso,Gisela Rodrigues da Silva, Florencio-Silva,Rinaldo, Baracat,Edmund Chada, Girão,Manoel Batista Castello, Soares Júnior,José Maria, Simões,Manuel de Jesus
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Associação Médica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000200174
Resumo: SUMMARY INTRODUCTION Although estrogen therapy is widely used against post-menopausal symptoms, it can present adverse effects, including endometrial cancer. Soy isoflavones are considered a possible alternative to estrogen therapy. However, there are still concerns whether isoflavones exert trophic effects on the uterine cervix. OBJECTIVES To evaluate the histomorphometric and immunohistochemical alterations in the uterine cervix of ovariectomized rats treated with soy isoflavones (Iso). METHODS Fifteen adult Wistar rats were ovariectomized (Ovx) and divided into three groups: Group I (Ovx), administered with vehicle solution; Group II (OVX-Iso), administered with concentrated extract of Iso (150 mg/kg) by gavage; and Group III (OVX-E2), treated with 17β-estradiol (10 µg/kg), subcutaneously. After 30 days of treatments, the uterine cervix was fixed in 10% formaldehyde and processed for paraffin-embedding. Sections were stained with Hematoxylin and eosin for morphological and morphometric studies or subjected to immunohistochemistry for detections of Ki-67 and vascular endothelial growth factor-A (Vegf-A). The data obtained were subjected to statistical analysis (p ≤ 0.05). RESULTS We noted an atrophic uterine cervix in GI, whereas it was more voluminous in GII and even more voluminous in GIII. The thickness of the cervical mucosa was significantly higher in GIII, as compared to GI and GII. The cell proliferation (Ki-67) was significantly elevated in the estradiol and isoflavones treated groups, whereas Vegf-A immunoexpression was significantly higher in GIII, as compared to groups GII and GI. CONCLUSIONS Soy isoflavones cause less trophic and proliferative effects in the uterine cervix of rats as compared to estrogen.
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spelling The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosaCervix uteriIsoflavonesEstrogensOvariectomyRatsSUMMARY INTRODUCTION Although estrogen therapy is widely used against post-menopausal symptoms, it can present adverse effects, including endometrial cancer. Soy isoflavones are considered a possible alternative to estrogen therapy. However, there are still concerns whether isoflavones exert trophic effects on the uterine cervix. OBJECTIVES To evaluate the histomorphometric and immunohistochemical alterations in the uterine cervix of ovariectomized rats treated with soy isoflavones (Iso). METHODS Fifteen adult Wistar rats were ovariectomized (Ovx) and divided into three groups: Group I (Ovx), administered with vehicle solution; Group II (OVX-Iso), administered with concentrated extract of Iso (150 mg/kg) by gavage; and Group III (OVX-E2), treated with 17β-estradiol (10 µg/kg), subcutaneously. After 30 days of treatments, the uterine cervix was fixed in 10% formaldehyde and processed for paraffin-embedding. Sections were stained with Hematoxylin and eosin for morphological and morphometric studies or subjected to immunohistochemistry for detections of Ki-67 and vascular endothelial growth factor-A (Vegf-A). The data obtained were subjected to statistical analysis (p ≤ 0.05). RESULTS We noted an atrophic uterine cervix in GI, whereas it was more voluminous in GII and even more voluminous in GIII. The thickness of the cervical mucosa was significantly higher in GIII, as compared to GI and GII. The cell proliferation (Ki-67) was significantly elevated in the estradiol and isoflavones treated groups, whereas Vegf-A immunoexpression was significantly higher in GIII, as compared to groups GII and GI. CONCLUSIONS Soy isoflavones cause less trophic and proliferative effects in the uterine cervix of rats as compared to estrogen.Associação Médica Brasileira2020-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302020000200174Revista da Associação Médica Brasileira v.66 n.2 2020reponame:Revista da Associação Médica Brasileira (Online)instname:Associação Médica Brasileira (AMB)instacron:AMB10.1590/1806-9282.66.2.174info:eu-repo/semantics/openAccessFranco,Paulo CelsoSimões,Ricardo SantosCarbonel,Adriana Aparecida FerrazSasso,Gisela Rodrigues da SilvaFlorencio-Silva,RinaldoBaracat,Edmund ChadaGirão,Manoel Batista CastelloSoares Júnior,José MariaSimões,Manuel de Jesuseng2020-05-11T00:00:00Zoai:scielo:S0104-42302020000200174Revistahttps://ramb.amb.org.br/ultimas-edicoes/#https://old.scielo.br/oai/scielo-oai.php||ramb@amb.org.br1806-92820104-4230opendoar:2020-05-11T00:00Revista da Associação Médica Brasileira (Online) - Associação Médica Brasileira (AMB)false
dc.title.none.fl_str_mv The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
title The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
spellingShingle The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
Franco,Paulo Celso
Cervix uteri
Isoflavones
Estrogens
Ovariectomy
Rats
title_short The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
title_full The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
title_fullStr The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
title_full_unstemmed The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
title_sort The influence of phytoestrogens or estrogens on the proliferation of the rat endocervical mucosa
author Franco,Paulo Celso
author_facet Franco,Paulo Celso
Simões,Ricardo Santos
Carbonel,Adriana Aparecida Ferraz
Sasso,Gisela Rodrigues da Silva
Florencio-Silva,Rinaldo
Baracat,Edmund Chada
Girão,Manoel Batista Castello
Soares Júnior,José Maria
Simões,Manuel de Jesus
author_role author
author2 Simões,Ricardo Santos
Carbonel,Adriana Aparecida Ferraz
Sasso,Gisela Rodrigues da Silva
Florencio-Silva,Rinaldo
Baracat,Edmund Chada
Girão,Manoel Batista Castello
Soares Júnior,José Maria
Simões,Manuel de Jesus
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Franco,Paulo Celso
Simões,Ricardo Santos
Carbonel,Adriana Aparecida Ferraz
Sasso,Gisela Rodrigues da Silva
Florencio-Silva,Rinaldo
Baracat,Edmund Chada
Girão,Manoel Batista Castello
Soares Júnior,José Maria
Simões,Manuel de Jesus
dc.subject.por.fl_str_mv Cervix uteri
Isoflavones
Estrogens
Ovariectomy
Rats
topic Cervix uteri
Isoflavones
Estrogens
Ovariectomy
Rats
description SUMMARY INTRODUCTION Although estrogen therapy is widely used against post-menopausal symptoms, it can present adverse effects, including endometrial cancer. Soy isoflavones are considered a possible alternative to estrogen therapy. However, there are still concerns whether isoflavones exert trophic effects on the uterine cervix. OBJECTIVES To evaluate the histomorphometric and immunohistochemical alterations in the uterine cervix of ovariectomized rats treated with soy isoflavones (Iso). METHODS Fifteen adult Wistar rats were ovariectomized (Ovx) and divided into three groups: Group I (Ovx), administered with vehicle solution; Group II (OVX-Iso), administered with concentrated extract of Iso (150 mg/kg) by gavage; and Group III (OVX-E2), treated with 17β-estradiol (10 µg/kg), subcutaneously. After 30 days of treatments, the uterine cervix was fixed in 10% formaldehyde and processed for paraffin-embedding. Sections were stained with Hematoxylin and eosin for morphological and morphometric studies or subjected to immunohistochemistry for detections of Ki-67 and vascular endothelial growth factor-A (Vegf-A). The data obtained were subjected to statistical analysis (p ≤ 0.05). RESULTS We noted an atrophic uterine cervix in GI, whereas it was more voluminous in GII and even more voluminous in GIII. The thickness of the cervical mucosa was significantly higher in GIII, as compared to GI and GII. The cell proliferation (Ki-67) was significantly elevated in the estradiol and isoflavones treated groups, whereas Vegf-A immunoexpression was significantly higher in GIII, as compared to groups GII and GI. CONCLUSIONS Soy isoflavones cause less trophic and proliferative effects in the uterine cervix of rats as compared to estrogen.
publishDate 2020
dc.date.none.fl_str_mv 2020-02-01
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dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 10.1590/1806-9282.66.2.174
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Associação Médica Brasileira
publisher.none.fl_str_mv Associação Médica Brasileira
dc.source.none.fl_str_mv Revista da Associação Médica Brasileira v.66 n.2 2020
reponame:Revista da Associação Médica Brasileira (Online)
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