Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | São Paulo medical journal (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009 |
Resumo: | CONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkins disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkins disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkins disease, and in all patients with HIV-associated Hodgkins disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status. |
id |
APM-1_b1a67ec5cf2449ab3bf9cbdb7ae06b41 |
---|---|
oai_identifier_str |
oai:scielo:S1516-31802006000300009 |
network_acronym_str |
APM-1 |
network_name_str |
São Paulo medical journal (Online) |
repository_id_str |
|
spelling |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins diseaseDNAEpstein-Barr virusHodgkin diseaseLymphomaPolymerase chain reactionCONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkins disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkins disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkins disease, and in all patients with HIV-associated Hodgkins disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status.Associação Paulista de Medicina - APM2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009Sao Paulo Medical Journal v.124 n.3 2006reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APM10.1590/S1516-31802006000300009info:eu-repo/semantics/openAccessMusacchio,Juliane GarcezCarvalho,Maria da Glória da CostaMorais,José Carlos Oliveira deSilva,Nathalie HenriquesScheliga,AdrianaRomano,SérgioSpector,Nelsoneng2006-09-13T00:00:00Zoai:scielo:S1516-31802006000300009Revistahttp://www.scielo.br/spmjhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2006-09-13T00:00São Paulo medical journal (Online) - Associação Paulista de Medicinafalse |
dc.title.none.fl_str_mv |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
title |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
spellingShingle |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease Musacchio,Juliane Garcez DNA Epstein-Barr virus Hodgkin disease Lymphoma Polymerase chain reaction |
title_short |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
title_full |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
title_fullStr |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
title_full_unstemmed |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
title_sort |
Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkins disease |
author |
Musacchio,Juliane Garcez |
author_facet |
Musacchio,Juliane Garcez Carvalho,Maria da Glória da Costa Morais,José Carlos Oliveira de Silva,Nathalie Henriques Scheliga,Adriana Romano,Sérgio Spector,Nelson |
author_role |
author |
author2 |
Carvalho,Maria da Glória da Costa Morais,José Carlos Oliveira de Silva,Nathalie Henriques Scheliga,Adriana Romano,Sérgio Spector,Nelson |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Musacchio,Juliane Garcez Carvalho,Maria da Glória da Costa Morais,José Carlos Oliveira de Silva,Nathalie Henriques Scheliga,Adriana Romano,Sérgio Spector,Nelson |
dc.subject.por.fl_str_mv |
DNA Epstein-Barr virus Hodgkin disease Lymphoma Polymerase chain reaction |
topic |
DNA Epstein-Barr virus Hodgkin disease Lymphoma Polymerase chain reaction |
description |
CONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkins disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkins disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkins disease, and in all patients with HIV-associated Hodgkins disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1516-31802006000300009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
publisher.none.fl_str_mv |
Associação Paulista de Medicina - APM |
dc.source.none.fl_str_mv |
Sao Paulo Medical Journal v.124 n.3 2006 reponame:São Paulo medical journal (Online) instname:Associação Paulista de Medicina instacron:APM |
instname_str |
Associação Paulista de Medicina |
instacron_str |
APM |
institution |
APM |
reponame_str |
São Paulo medical journal (Online) |
collection |
São Paulo medical journal (Online) |
repository.name.fl_str_mv |
São Paulo medical journal (Online) - Associação Paulista de Medicina |
repository.mail.fl_str_mv |
revistas@apm.org.br |
_version_ |
1754209261654638592 |