Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease

Detalhes bibliográficos
Autor(a) principal: Musacchio,Juliane Garcez
Data de Publicação: 2006
Outros Autores: Carvalho,Maria da Glória da Costa, Morais,José Carlos Oliveira de, Silva,Nathalie Henriques, Scheliga,Adriana, Romano,Sérgio, Spector,Nelson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: São Paulo medical journal (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009
Resumo: CONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkin’s disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkin’s disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkin’s disease, and in all patients with HIV-associated Hodgkin’s disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status.
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spelling Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s diseaseDNAEpstein-Barr virusHodgkin diseaseLymphomaPolymerase chain reactionCONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkin’s disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkin’s disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkin’s disease, and in all patients with HIV-associated Hodgkin’s disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status.Associação Paulista de Medicina - APM2006-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009Sao Paulo Medical Journal v.124 n.3 2006reponame:São Paulo medical journal (Online)instname:Associação Paulista de Medicinainstacron:APM10.1590/S1516-31802006000300009info:eu-repo/semantics/openAccessMusacchio,Juliane GarcezCarvalho,Maria da Glória da CostaMorais,José Carlos Oliveira deSilva,Nathalie HenriquesScheliga,AdrianaRomano,SérgioSpector,Nelsoneng2006-09-13T00:00:00Zoai:scielo:S1516-31802006000300009Revistahttp://www.scielo.br/spmjhttps://old.scielo.br/oai/scielo-oai.phprevistas@apm.org.br1806-94601516-3180opendoar:2006-09-13T00:00São Paulo medical journal (Online) - Associação Paulista de Medicinafalse
dc.title.none.fl_str_mv Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
title Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
spellingShingle Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
Musacchio,Juliane Garcez
DNA
Epstein-Barr virus
Hodgkin disease
Lymphoma
Polymerase chain reaction
title_short Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
title_full Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
title_fullStr Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
title_full_unstemmed Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
title_sort Detection of free circulating Epstein-Barr virus DNA in plasma of patients with Hodgkin’s disease
author Musacchio,Juliane Garcez
author_facet Musacchio,Juliane Garcez
Carvalho,Maria da Glória da Costa
Morais,José Carlos Oliveira de
Silva,Nathalie Henriques
Scheliga,Adriana
Romano,Sérgio
Spector,Nelson
author_role author
author2 Carvalho,Maria da Glória da Costa
Morais,José Carlos Oliveira de
Silva,Nathalie Henriques
Scheliga,Adriana
Romano,Sérgio
Spector,Nelson
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Musacchio,Juliane Garcez
Carvalho,Maria da Glória da Costa
Morais,José Carlos Oliveira de
Silva,Nathalie Henriques
Scheliga,Adriana
Romano,Sérgio
Spector,Nelson
dc.subject.por.fl_str_mv DNA
Epstein-Barr virus
Hodgkin disease
Lymphoma
Polymerase chain reaction
topic DNA
Epstein-Barr virus
Hodgkin disease
Lymphoma
Polymerase chain reaction
description CONTEXT AND OBJECTIVE: Free circulating Epstein-Barr virus (EBV) DNA is often present in the plasma of Hodgkin’s disease patients. The aim here was to evaluate the prevalence of this finding, its correlation with the immunohistochemical expression of LMP-1 (latent membrane protein 1) and the influence of other clinical factors. DESIGN AND SETTING: Prospective study in two public tertiary institutions: Hematology Service, Universidade Federal do Rio de Janeiro, and Oncology Service, Instituto Nacional do Câncer, Rio de Janeiro. METHODS: A cohort of 30 patients with newly diagnosed Hodgkin’s disease was studied. The control group consisted of 13 healthy adult volunteers. EBV DNA was determined by conventional polymerase chain reaction (PCR). RESULTS: The median age was 28 years, and 16 patients were women. Advanced disease was present in 19 patients, and six were HIV-positive. EBV DNA was present in the plasma of 13 patients and one control (43% versus 8%, p = 0.03). EBV DNA prevalence was higher in HIV-positive patients (100% versus 29%, p = 0.0007) and those with advanced disease (63% versus 9%, p = 0.006). Among HIV-negative patients alone, EBV DNA prevalence remained higher in those with advanced disease. EBV DNA was found in 10/11 patients with LMP-1 expression in the lymph nodes, and in 3/19 without LMP-1 expression (kappa coefficient = 0.72). CONCLUSION: EBV DNA was present in 91% of patients with EBV-associated Hodgkin’s disease, and in all patients with HIV-associated Hodgkin’s disease. EBV DNA prevalence was higher in patients with advanced disease, irrespective of HIV status.
publishDate 2006
dc.date.none.fl_str_mv 2006-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-31802006000300009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-31802006000300009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Associação Paulista de Medicina - APM
publisher.none.fl_str_mv Associação Paulista de Medicina - APM
dc.source.none.fl_str_mv Sao Paulo Medical Journal v.124 n.3 2006
reponame:São Paulo medical journal (Online)
instname:Associação Paulista de Medicina
instacron:APM
instname_str Associação Paulista de Medicina
instacron_str APM
institution APM
reponame_str São Paulo medical journal (Online)
collection São Paulo medical journal (Online)
repository.name.fl_str_mv São Paulo medical journal (Online) - Associação Paulista de Medicina
repository.mail.fl_str_mv revistas@apm.org.br
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