RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/43673 |
Resumo: | Objective: Osteoradionecrosis (ORN) of the jaws is an important side effect of head and neck cancer radiotherapy. The main objective of this work was to evaluate the expression of RANK, RANKL and OPG proteins, markers of bone remodeling, in rats submitted to radiotherapy. Material and Methods: Six-month-old male Wistar rats were divided into two groups: Irradiated group (IR), which received a single dose of 20 Gy in the jaw and after seven days had their three left mandibular molars extracted; Control group (C), which didn’t receive radiation, but underwent the same procedures. Twenty one days after the dental extractions, the animals were euthanized and their mandibles were removed for analysis. The volume density (Vv) of immunoexpression of RANK/RANKL/OPG was quantified by stereology. The bone volume ratio (BV/TV), the trabecular thickness (Tb.Th), the trabecular separation (Tb.Sp) and the trabecular number (Tb.N) were analyzed by microtomography (Micro-CT). Results: Vv of immunoexpression of RANKL and OPG were, respectively, 53% higher and 50% lower in IR animals compared to C group (p<0.05). Micro-CT showed that in the IR animals there was a reduction of the bone mass compared to the C animals. BV/TV and Tb.Th were 33% and 38% lower, respectively, in the IR animals (p<0.05), while Tb.Sp was 37.5% higher in these animals (p<0.05). Conclusions: Ionizing irradiation in a single high dose, followed by dental extractions promoted ORN in mandibular bone in rats, together with changes in the markers of bone remodeling RANK and OPG. |
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Brazilian Journal of Health Review |
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RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibularRadiotherapyOsteoradionecrosisBone remodelingRANKLOPG.Objective: Osteoradionecrosis (ORN) of the jaws is an important side effect of head and neck cancer radiotherapy. The main objective of this work was to evaluate the expression of RANK, RANKL and OPG proteins, markers of bone remodeling, in rats submitted to radiotherapy. Material and Methods: Six-month-old male Wistar rats were divided into two groups: Irradiated group (IR), which received a single dose of 20 Gy in the jaw and after seven days had their three left mandibular molars extracted; Control group (C), which didn’t receive radiation, but underwent the same procedures. Twenty one days after the dental extractions, the animals were euthanized and their mandibles were removed for analysis. The volume density (Vv) of immunoexpression of RANK/RANKL/OPG was quantified by stereology. The bone volume ratio (BV/TV), the trabecular thickness (Tb.Th), the trabecular separation (Tb.Sp) and the trabecular number (Tb.N) were analyzed by microtomography (Micro-CT). Results: Vv of immunoexpression of RANKL and OPG were, respectively, 53% higher and 50% lower in IR animals compared to C group (p<0.05). Micro-CT showed that in the IR animals there was a reduction of the bone mass compared to the C animals. BV/TV and Tb.Th were 33% and 38% lower, respectively, in the IR animals (p<0.05), while Tb.Sp was 37.5% higher in these animals (p<0.05). Conclusions: Ionizing irradiation in a single high dose, followed by dental extractions promoted ORN in mandibular bone in rats, together with changes in the markers of bone remodeling RANK and OPG.Brazilian Journals Publicações de Periódicos e Editora Ltda.2022-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4367310.34119/bjhrv5n1-199Brazilian Journal of Health Review; Vol. 5 No. 1 (2022); 2258-2276Brazilian Journal of Health Review; v. 5 n. 1 (2022); 2258-22762595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/43673/pdfCopyright (c) 2022 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessdos Santos, Jefferson FerreiraFerreira-Machado, Samara CristinaSalata, CamilaNogueira, Liebert ParreirasLau, Claudio Sérgio CorreaAppoloni, Carlos RobertoTorres, Thiago da Silva2022-02-28T20:37:27Zoai:ojs2.ojs.brazilianjournals.com.br:article/43673Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2022-02-28T20:37:27Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
title |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
spellingShingle |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular dos Santos, Jefferson Ferreira Radiotherapy Osteoradionecrosis Bone remodeling RANKL OPG. |
title_short |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
title_full |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
title_fullStr |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
title_full_unstemmed |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
title_sort |
RANKL/OPG signaling affects the bone structure in rat model of mandibular osteoradionecrosis / A sinalização RANKL/OPG acomete estrutura óssea em modelo de rato de osteorradionecrose mandibular |
author |
dos Santos, Jefferson Ferreira |
author_facet |
dos Santos, Jefferson Ferreira Ferreira-Machado, Samara Cristina Salata, Camila Nogueira, Liebert Parreiras Lau, Claudio Sérgio Correa Appoloni, Carlos Roberto Torres, Thiago da Silva |
author_role |
author |
author2 |
Ferreira-Machado, Samara Cristina Salata, Camila Nogueira, Liebert Parreiras Lau, Claudio Sérgio Correa Appoloni, Carlos Roberto Torres, Thiago da Silva |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
dos Santos, Jefferson Ferreira Ferreira-Machado, Samara Cristina Salata, Camila Nogueira, Liebert Parreiras Lau, Claudio Sérgio Correa Appoloni, Carlos Roberto Torres, Thiago da Silva |
dc.subject.por.fl_str_mv |
Radiotherapy Osteoradionecrosis Bone remodeling RANKL OPG. |
topic |
Radiotherapy Osteoradionecrosis Bone remodeling RANKL OPG. |
description |
Objective: Osteoradionecrosis (ORN) of the jaws is an important side effect of head and neck cancer radiotherapy. The main objective of this work was to evaluate the expression of RANK, RANKL and OPG proteins, markers of bone remodeling, in rats submitted to radiotherapy. Material and Methods: Six-month-old male Wistar rats were divided into two groups: Irradiated group (IR), which received a single dose of 20 Gy in the jaw and after seven days had their three left mandibular molars extracted; Control group (C), which didn’t receive radiation, but underwent the same procedures. Twenty one days after the dental extractions, the animals were euthanized and their mandibles were removed for analysis. The volume density (Vv) of immunoexpression of RANK/RANKL/OPG was quantified by stereology. The bone volume ratio (BV/TV), the trabecular thickness (Tb.Th), the trabecular separation (Tb.Sp) and the trabecular number (Tb.N) were analyzed by microtomography (Micro-CT). Results: Vv of immunoexpression of RANKL and OPG were, respectively, 53% higher and 50% lower in IR animals compared to C group (p<0.05). Micro-CT showed that in the IR animals there was a reduction of the bone mass compared to the C animals. BV/TV and Tb.Th were 33% and 38% lower, respectively, in the IR animals (p<0.05), while Tb.Sp was 37.5% higher in these animals (p<0.05). Conclusions: Ionizing irradiation in a single high dose, followed by dental extractions promoted ORN in mandibular bone in rats, together with changes in the markers of bone remodeling RANK and OPG. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-02-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/43673 10.34119/bjhrv5n1-199 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/43673 |
identifier_str_mv |
10.34119/bjhrv5n1-199 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/43673/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Health Review info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Health Review |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 5 No. 1 (2022); 2258-2276 Brazilian Journal of Health Review; v. 5 n. 1 (2022); 2258-2276 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
_version_ |
1797240071900889088 |