Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493 |
Resumo: | Background: Enteroparasites infections remains an important health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. |
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Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/BrazilBlastocystis sppE. histolytica/E. dispardiagnosisBackground: Enteroparasites infections remains an important health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. Brazilian Journals Publicações de Periódicos e Editora Ltda.2021-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/3049310.34119/bjhrv4n3-163Brazilian Journal of Health Review; Vol. 4 No. 3 (2021); 11741-11754Brazilian Journal of Health Review; v. 4 n. 3 (2021); 11741-117542595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493/pdfCopyright (c) 2021 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessBelchior, Vera Lucia ReisToma, Helena KeikoBatista, Keila MoreiraBernarde, Karine Ingrid De Souzade Carvalho, Laís GonçalvesPilotto, Thiago PimentelAdami, Yara LeiteXavier, Analucia Rampazzo2021-07-01T20:07:13Zoai:ojs2.ojs.brazilianjournals.com.br:article/30493Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2021-07-01T20:07:13Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
title |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
spellingShingle |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil Belchior, Vera Lucia Reis Blastocystis spp E. histolytica/E. dispar diagnosis |
title_short |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
title_full |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
title_fullStr |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
title_full_unstemmed |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
title_sort |
Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil |
author |
Belchior, Vera Lucia Reis |
author_facet |
Belchior, Vera Lucia Reis Toma, Helena Keiko Batista, Keila Moreira Bernarde, Karine Ingrid De Souza de Carvalho, Laís Gonçalves Pilotto, Thiago Pimentel Adami, Yara Leite Xavier, Analucia Rampazzo |
author_role |
author |
author2 |
Toma, Helena Keiko Batista, Keila Moreira Bernarde, Karine Ingrid De Souza de Carvalho, Laís Gonçalves Pilotto, Thiago Pimentel Adami, Yara Leite Xavier, Analucia Rampazzo |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Belchior, Vera Lucia Reis Toma, Helena Keiko Batista, Keila Moreira Bernarde, Karine Ingrid De Souza de Carvalho, Laís Gonçalves Pilotto, Thiago Pimentel Adami, Yara Leite Xavier, Analucia Rampazzo |
dc.subject.por.fl_str_mv |
Blastocystis spp E. histolytica/E. dispar diagnosis |
topic |
Blastocystis spp E. histolytica/E. dispar diagnosis |
description |
Background: Enteroparasites infections remains an important health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-28 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493 10.34119/bjhrv4n3-163 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493 |
identifier_str_mv |
10.34119/bjhrv4n3-163 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Brazilian Journal of Health Review info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Brazilian Journal of Health Review |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 4 No. 3 (2021); 11741-11754 Brazilian Journal of Health Review; v. 4 n. 3 (2021); 11741-11754 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
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1797240063205048320 |