Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil

Detalhes bibliográficos
Autor(a) principal: Belchior, Vera Lucia Reis
Data de Publicação: 2021
Outros Autores: Toma, Helena Keiko, Batista, Keila Moreira, Bernarde, Karine Ingrid De Souza, de Carvalho, Laís Gonçalves, Pilotto, Thiago Pimentel, Adami, Yara Leite, Xavier, Analucia Rampazzo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Health Review
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493
Resumo: Background: Enteroparasites infections remains an important  health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai  techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. 
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spelling Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/BrazilBlastocystis sppE. histolytica/E. dispardiagnosisBackground: Enteroparasites infections remains an important  health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai  techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. Brazilian Journals Publicações de Periódicos e Editora Ltda.2021-05-28info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/3049310.34119/bjhrv4n3-163Brazilian Journal of Health Review; Vol. 4 No. 3 (2021); 11741-11754Brazilian Journal of Health Review; v. 4 n. 3 (2021); 11741-117542595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493/pdfCopyright (c) 2021 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessBelchior, Vera Lucia ReisToma, Helena KeikoBatista, Keila MoreiraBernarde, Karine Ingrid De Souzade Carvalho, Laís GonçalvesPilotto, Thiago PimentelAdami, Yara LeiteXavier, Analucia Rampazzo2021-07-01T20:07:13Zoai:ojs2.ojs.brazilianjournals.com.br:article/30493Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2021-07-01T20:07:13Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false
dc.title.none.fl_str_mv Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
title Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
spellingShingle Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
Belchior, Vera Lucia Reis
Blastocystis spp
E. histolytica/E. dispar
diagnosis
title_short Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
title_full Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
title_fullStr Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
title_full_unstemmed Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
title_sort Predominance of Blastocystis spp, entamoeba histolytica /e.dispar and other protozoan evaluated through classical and molecular diagnostic techniques in two communities of Niterói-rj/Brazil. / Predominância de Blastocystis spp, entamoeba histolytica /e.dispar e outros protozoários avaliados através de técnicas de diagnóstico clássico e molecular em duas comunidades de Niterói-rj/Brazil
author Belchior, Vera Lucia Reis
author_facet Belchior, Vera Lucia Reis
Toma, Helena Keiko
Batista, Keila Moreira
Bernarde, Karine Ingrid De Souza
de Carvalho, Laís Gonçalves
Pilotto, Thiago Pimentel
Adami, Yara Leite
Xavier, Analucia Rampazzo
author_role author
author2 Toma, Helena Keiko
Batista, Keila Moreira
Bernarde, Karine Ingrid De Souza
de Carvalho, Laís Gonçalves
Pilotto, Thiago Pimentel
Adami, Yara Leite
Xavier, Analucia Rampazzo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Belchior, Vera Lucia Reis
Toma, Helena Keiko
Batista, Keila Moreira
Bernarde, Karine Ingrid De Souza
de Carvalho, Laís Gonçalves
Pilotto, Thiago Pimentel
Adami, Yara Leite
Xavier, Analucia Rampazzo
dc.subject.por.fl_str_mv Blastocystis spp
E. histolytica/E. dispar
diagnosis
topic Blastocystis spp
E. histolytica/E. dispar
diagnosis
description Background: Enteroparasites infections remains an important  health issue in Brazil as contaminations are still detected among inhabitants of socially vulnerable populations of the country. This study was performed in two low income communities in Niterói, Rio de Janeiro State, Brazil. Methods: Health educational actions in two selected communities were performed and in parallel, 150 stool samples were collected. Coproparasitological diagnosis was carried out employing Hoffmann, Pons & Janer , Willis and Rugai  techniques 5,6,7. Molecular diagnosis using Paglia & Visca protocol was performed to detect and differentiate E. histolytica/E.dispar infections 8. Results: Enteroparasites were found in more than 50% of the samples and protozoan infections prevailed. Blastocystis spp was the most prevalent protozoan (55.08%) followed by Endolimax nana (16.10%), Giardia lamblia (15.56%) and Entamoeba coli (11.02%). For helminth species, only Enterobius vermicularis and Strongyloides stercoralis were found, in 4.24% of positive samples. All samples were negative for E.histolytica/E. dispar infections through coproscopy, however, PCR analysis of 143 suitable stool samples showed that both species were present - as a complex or not - in 15% of the samples, in both institutions evaluated. Conclusions: The high frequency of protozoan infections may indicate extensive faecal-oral contamination. Additionally, it can ensure the basis for directed intervention actions in some communities to prevent water transmitted parasites providing sanitary infrastructure. Also, educational health programs for inhabitants of low income communities of Niterói, Rio de Janeiro State, Brazil, are necessary in order to provide a better understanding of enteroparasites and its prophylatic measures. 
publishDate 2021
dc.date.none.fl_str_mv 2021-05-28
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493
10.34119/bjhrv4n3-163
url https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493
identifier_str_mv 10.34119/bjhrv4n3-163
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/30493/pdf
dc.rights.driver.fl_str_mv Copyright (c) 2021 Brazilian Journal of Health Review
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2021 Brazilian Journal of Health Review
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Health Review; Vol. 4 No. 3 (2021); 11741-11754
Brazilian Journal of Health Review; v. 4 n. 3 (2021); 11741-11754
2595-6825
reponame:Brazilian Journal of Health Review
instname:Federação das Indústrias do Estado do Paraná (FIEP)
instacron:BJRH
instname_str Federação das Indústrias do Estado do Paraná (FIEP)
instacron_str BJRH
institution BJRH
reponame_str Brazilian Journal of Health Review
collection Brazilian Journal of Health Review
repository.name.fl_str_mv Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)
repository.mail.fl_str_mv || brazilianjhr@gmail.com
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