Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/44735 |
Resumo: | The representation of the gene content of an organism is impacted by several factors, ranging from sampling to sequencing and then the genome assembly task. The genome assembly process can generate errors that are related to insufficient coverage in the data set, an inadequate assembly methodology, and finally, errors related to the limitation of the assembly software used. Thus, some genes remain unidentified both in complete and draft genomes, this incomplete gene knowledge impacts on several organisms, mainly of medical and industrial interest, such as Bifidobacterium breve, a Gram-positive bacterium, found in the gastrointestinal microbiota of mammals, including humans, and has beneficial probiotic activities. Therefore, the objective of this work is to identify the new gene products not represented in the genome of Bifidobacterium breve DS15-17 using the raw reads of this organism. The reads were produced from the sequencing with the Illumina MiSeq platform. PAN2HGENE software was used to identify new gene products. After the analysis, 44 new gene products were identified, 26 with described function and 18 hypothetical proteins. The hypothetical proteins identified were analyzed in the ProtoNet and Superfamily databases. |
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Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In SilicoBifidobacterium breveprobioticannotationnew productsgenome.The representation of the gene content of an organism is impacted by several factors, ranging from sampling to sequencing and then the genome assembly task. The genome assembly process can generate errors that are related to insufficient coverage in the data set, an inadequate assembly methodology, and finally, errors related to the limitation of the assembly software used. Thus, some genes remain unidentified both in complete and draft genomes, this incomplete gene knowledge impacts on several organisms, mainly of medical and industrial interest, such as Bifidobacterium breve, a Gram-positive bacterium, found in the gastrointestinal microbiota of mammals, including humans, and has beneficial probiotic activities. Therefore, the objective of this work is to identify the new gene products not represented in the genome of Bifidobacterium breve DS15-17 using the raw reads of this organism. The reads were produced from the sequencing with the Illumina MiSeq platform. PAN2HGENE software was used to identify new gene products. After the analysis, 44 new gene products were identified, 26 with described function and 18 hypothetical proteins. The hypothetical proteins identified were analyzed in the ProtoNet and Superfamily databases.Brazilian Journals Publicações de Periódicos e Editora Ltda.2022-03-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/4473510.34119/bjhrv5n2-012Brazilian Journal of Health Review; Vol. 5 No. 2 (2022); 4141-4159Brazilian Journal of Health Review; v. 5 n. 2 (2022); 4141-41592595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHenghttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/44735/pdfCopyright (c) 2022 Brazilian Journal of Health Reviewinfo:eu-repo/semantics/openAccessDamasceno, BiancaSantos, RonilsonOliveira, MônicaDias, LarissaLima, AlyneVeras, Adonney AllanSá, Pablo deAlves, Jorianne2022-05-02T19:42:51Zoai:ojs2.ojs.brazilianjournals.com.br:article/44735Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2022-05-02T19:42:51Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
title |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
spellingShingle |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico Damasceno, Bianca Bifidobacterium breve probiotic annotation new products genome. |
title_short |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
title_full |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
title_fullStr |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
title_full_unstemmed |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
title_sort |
Prediction of new gene products and characterization of hypothetical proteins of Bifidobacterium breve DS15-17 In Silico / Predição de novos produtos gênicos e caracterização de proteínas hipotéticas de Bifidobacterium breve DS15-17 In Silico |
author |
Damasceno, Bianca |
author_facet |
Damasceno, Bianca Santos, Ronilson Oliveira, Mônica Dias, Larissa Lima, Alyne Veras, Adonney Allan Sá, Pablo de Alves, Jorianne |
author_role |
author |
author2 |
Santos, Ronilson Oliveira, Mônica Dias, Larissa Lima, Alyne Veras, Adonney Allan Sá, Pablo de Alves, Jorianne |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Damasceno, Bianca Santos, Ronilson Oliveira, Mônica Dias, Larissa Lima, Alyne Veras, Adonney Allan Sá, Pablo de Alves, Jorianne |
dc.subject.por.fl_str_mv |
Bifidobacterium breve probiotic annotation new products genome. |
topic |
Bifidobacterium breve probiotic annotation new products genome. |
description |
The representation of the gene content of an organism is impacted by several factors, ranging from sampling to sequencing and then the genome assembly task. The genome assembly process can generate errors that are related to insufficient coverage in the data set, an inadequate assembly methodology, and finally, errors related to the limitation of the assembly software used. Thus, some genes remain unidentified both in complete and draft genomes, this incomplete gene knowledge impacts on several organisms, mainly of medical and industrial interest, such as Bifidobacterium breve, a Gram-positive bacterium, found in the gastrointestinal microbiota of mammals, including humans, and has beneficial probiotic activities. Therefore, the objective of this work is to identify the new gene products not represented in the genome of Bifidobacterium breve DS15-17 using the raw reads of this organism. The reads were produced from the sequencing with the Illumina MiSeq platform. PAN2HGENE software was used to identify new gene products. After the analysis, 44 new gene products were identified, 26 with described function and 18 hypothetical proteins. The hypothetical proteins identified were analyzed in the ProtoNet and Superfamily databases. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-03 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/44735 10.34119/bjhrv5n2-012 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/44735 |
identifier_str_mv |
10.34119/bjhrv5n2-012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/44735/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Health Review info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Health Review |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 5 No. 2 (2022); 4141-4159 Brazilian Journal of Health Review; v. 5 n. 2 (2022); 4141-4159 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
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1797240072870821888 |