Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
Autor(a) principal: | |
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Data de Publicação: | 2024 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Health Review |
Texto Completo: | https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982 |
Resumo: | Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway. |
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Brazilian Journal of Health Review |
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Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathwayResveratrolAMPKdiabetes mellitus 2Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway.Brazilian Journals Publicações de Periódicos e Editora Ltda.2024-03-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/6798210.34119/bjhrv7n2-080Brazilian Journal of Health Review; Vol. 7 No. 2 (2024); e67982Brazilian Journal of Health Review; Vol. 7 Núm. 2 (2024); e67982Brazilian Journal of Health Review; v. 7 n. 2 (2024); e679822595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982/48415Suffi, NatháliaFranco, Filipe NogueiraChaves, Miriam MartinsAraújo, Glaucy Rodrigues deinfo:eu-repo/semantics/openAccess2024-03-15T14:11:51Zoai:ojs2.ojs.brazilianjournals.com.br:article/67982Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2024-03-15T14:11:51Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false |
dc.title.none.fl_str_mv |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
title |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
spellingShingle |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway Suffi, Nathália Resveratrol AMPK diabetes mellitus 2 |
title_short |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
title_full |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
title_fullStr |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
title_full_unstemmed |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
title_sort |
Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway |
author |
Suffi, Nathália |
author_facet |
Suffi, Nathália Franco, Filipe Nogueira Chaves, Miriam Martins Araújo, Glaucy Rodrigues de |
author_role |
author |
author2 |
Franco, Filipe Nogueira Chaves, Miriam Martins Araújo, Glaucy Rodrigues de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Suffi, Nathália Franco, Filipe Nogueira Chaves, Miriam Martins Araújo, Glaucy Rodrigues de |
dc.subject.por.fl_str_mv |
Resveratrol AMPK diabetes mellitus 2 |
topic |
Resveratrol AMPK diabetes mellitus 2 |
description |
Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982 10.34119/bjhrv7n2-080 |
url |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982 |
identifier_str_mv |
10.34119/bjhrv7n2-080 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982/48415 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
publisher.none.fl_str_mv |
Brazilian Journals Publicações de Periódicos e Editora Ltda. |
dc.source.none.fl_str_mv |
Brazilian Journal of Health Review; Vol. 7 No. 2 (2024); e67982 Brazilian Journal of Health Review; Vol. 7 Núm. 2 (2024); e67982 Brazilian Journal of Health Review; v. 7 n. 2 (2024); e67982 2595-6825 reponame:Brazilian Journal of Health Review instname:Federação das Indústrias do Estado do Paraná (FIEP) instacron:BJRH |
instname_str |
Federação das Indústrias do Estado do Paraná (FIEP) |
instacron_str |
BJRH |
institution |
BJRH |
reponame_str |
Brazilian Journal of Health Review |
collection |
Brazilian Journal of Health Review |
repository.name.fl_str_mv |
Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP) |
repository.mail.fl_str_mv |
|| brazilianjhr@gmail.com |
_version_ |
1797240044103139328 |