Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway

Detalhes bibliográficos
Autor(a) principal: Suffi, Nathália
Data de Publicação: 2024
Outros Autores: Franco, Filipe Nogueira, Chaves, Miriam Martins, Araújo, Glaucy Rodrigues de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Health Review
Texto Completo: https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982
Resumo: Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway.
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spelling Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathwayResveratrolAMPKdiabetes mellitus 2Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway.Brazilian Journals Publicações de Periódicos e Editora Ltda.2024-03-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/6798210.34119/bjhrv7n2-080Brazilian Journal of Health Review; Vol. 7 No. 2 (2024); e67982Brazilian Journal of Health Review; Vol. 7 Núm. 2 (2024); e67982Brazilian Journal of Health Review; v. 7 n. 2 (2024); e679822595-6825reponame:Brazilian Journal of Health Reviewinstname:Federação das Indústrias do Estado do Paraná (FIEP)instacron:BJRHporhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982/48415Suffi, NatháliaFranco, Filipe NogueiraChaves, Miriam MartinsAraújo, Glaucy Rodrigues deinfo:eu-repo/semantics/openAccess2024-03-15T14:11:51Zoai:ojs2.ojs.brazilianjournals.com.br:article/67982Revistahttp://www.brazilianjournals.com/index.php/BJHR/indexPRIhttps://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/oai|| brazilianjhr@gmail.com2595-68252595-6825opendoar:2024-03-15T14:11:51Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)false
dc.title.none.fl_str_mv Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
title Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
spellingShingle Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
Suffi, Nathália
Resveratrol
AMPK
diabetes mellitus 2
title_short Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
title_full Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
title_fullStr Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
title_full_unstemmed Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
title_sort Resveratrol acts as an antioxidant in leukocytes of patients with diabetes mellitus 2 through the AMPK signaling pathway
author Suffi, Nathália
author_facet Suffi, Nathália
Franco, Filipe Nogueira
Chaves, Miriam Martins
Araújo, Glaucy Rodrigues de
author_role author
author2 Franco, Filipe Nogueira
Chaves, Miriam Martins
Araújo, Glaucy Rodrigues de
author2_role author
author
author
dc.contributor.author.fl_str_mv Suffi, Nathália
Franco, Filipe Nogueira
Chaves, Miriam Martins
Araújo, Glaucy Rodrigues de
dc.subject.por.fl_str_mv Resveratrol
AMPK
diabetes mellitus 2
topic Resveratrol
AMPK
diabetes mellitus 2
description Diabetes is characterized by disturbances in serum glucose levels caused by insulin resistance, with consequent high blood glucose levels. Permanently increased blood glucose tends to lead to an increase in reactive oxygen species (ROS). Therefore, one of the mechanisms to contain this process is to consider antioxidant treatment. Within this context, resveratrol (RSV) stands out. Among the pathways of action of this compound, AMPK plays a fundamental role in regulating its antioxidant activity. The objective of the work aims to verify the antioxidant profile of RSV, through the AMPK pathway, in leukocytes of patients with type 2 diabetes mellitus (DM2) between 60 and 80 years old. 12 individuals were selected, divided into two groups: control group (n=6) and experimental group (n=6). After blood collection and leukocyte separation, the luminol-dependent chemiluminescence assay was performed. To analyze the involvement of the AMPK pathway, the inhibitor compound C was used. The result obtained showed that there was an antioxidant behavior of AMPK in cells stimulated with peroxide and treated with RSV, this in turn decreased the production of ROS in leukocytes of all samples, but this effect was less significant in DM2 patients. This oxidation, which is more pronounced in diabetics, can lead to target organ damage. As RSV played an important role in reducing ROS, we concluded that it is an important compound in preventing target organ damage in patients with this comorbidity, acting through the AMPK pathway.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-11
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982
10.34119/bjhrv7n2-080
url https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982
identifier_str_mv 10.34119/bjhrv7n2-080
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ojs.brazilianjournals.com.br/ojs/index.php/BJHR/article/view/67982/48415
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
publisher.none.fl_str_mv Brazilian Journals Publicações de Periódicos e Editora Ltda.
dc.source.none.fl_str_mv Brazilian Journal of Health Review; Vol. 7 No. 2 (2024); e67982
Brazilian Journal of Health Review; Vol. 7 Núm. 2 (2024); e67982
Brazilian Journal of Health Review; v. 7 n. 2 (2024); e67982
2595-6825
reponame:Brazilian Journal of Health Review
instname:Federação das Indústrias do Estado do Paraná (FIEP)
instacron:BJRH
instname_str Federação das Indústrias do Estado do Paraná (FIEP)
instacron_str BJRH
institution BJRH
reponame_str Brazilian Journal of Health Review
collection Brazilian Journal of Health Review
repository.name.fl_str_mv Brazilian Journal of Health Review - Federação das Indústrias do Estado do Paraná (FIEP)
repository.mail.fl_str_mv || brazilianjhr@gmail.com
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