Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection

Detalhes bibliográficos
Autor(a) principal: Paulini,Inarei
Data de Publicação: 2017
Outros Autores: Siqueira-Silva,Joselma, Thomaz,Luciana, Rocha,Leticia, Harsi,Charlotte, Bellei,Nancy, Granato,Celso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500
Resumo: Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2, 3, 5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice.
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spelling Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infectionAdenovirusMonoclonal antibodiesImmunochromatographic testAbstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2, 3, 5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice.Brazilian Society of Infectious Diseases2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500Brazilian Journal of Infectious Diseases v.21 n.5 2017reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2017.03.023info:eu-repo/semantics/openAccessPaulini,InareiSiqueira-Silva,JoselmaThomaz,LucianaRocha,LeticiaHarsi,CharlotteBellei,NancyGranato,Celsoeng2017-10-23T00:00:00Zoai:scielo:S1413-86702017000500500Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2017-10-23T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
title Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
spellingShingle Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
Paulini,Inarei
Adenovirus
Monoclonal antibodies
Immunochromatographic test
title_short Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
title_full Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
title_fullStr Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
title_full_unstemmed Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
title_sort Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection
author Paulini,Inarei
author_facet Paulini,Inarei
Siqueira-Silva,Joselma
Thomaz,Luciana
Rocha,Leticia
Harsi,Charlotte
Bellei,Nancy
Granato,Celso
author_role author
author2 Siqueira-Silva,Joselma
Thomaz,Luciana
Rocha,Leticia
Harsi,Charlotte
Bellei,Nancy
Granato,Celso
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paulini,Inarei
Siqueira-Silva,Joselma
Thomaz,Luciana
Rocha,Leticia
Harsi,Charlotte
Bellei,Nancy
Granato,Celso
dc.subject.por.fl_str_mv Adenovirus
Monoclonal antibodies
Immunochromatographic test
topic Adenovirus
Monoclonal antibodies
Immunochromatographic test
description Abstract Human adenoviruses comprise an important group of etiologic agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2, 3, 5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500500
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjid.2017.03.023
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.21 n.5 2017
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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