Comparative study of adenoviruses with monoclonal antibodies

Detalhes bibliográficos
Autor(a) principal: Paiva, Terezinha Maria de
Data de Publicação: 1992
Outros Autores: Takimoto, Sueko, Ishida, María Akíko, Souza, María Candida Oliveira de, Ishimaru, Tuneo, Neumann, Jorge, Kalil, Jorge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista do Instituto de Medicina Tropical de São Paulo
Texto Completo: https://www.revistas.usp.br/rimtsp/article/view/28886
Resumo: The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.
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spelling Comparative study of adenoviruses with monoclonal antibodies Estudo comparativo de diferentes tipos de adenovirus através de anticorpos monoclonais AdenovirusSpecies SpecificMonoclonal antibodiesAdenovirus 4Antigenic Relationship The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained. O estudo relata a obtenção de anticorpos monoclonais para o adenovirus tipo (espécie) 4. A especificidade dos anticorpos monoclonais, selecionados nesse experimento, foi determinada testando-os frente às diferentes espécies de adenovírus: 1, 2, 5 (subgênero C), 4 (subgênero E), 7 e 16 (subgênero B) e 9 (subgênero D), pelas técnicas de neutralização em cultura de células, imunofluorescência e ensaio imunoenzimático (ELISA). Os resultados demonstram a obtenção de anticorpos monoclonais que reagiram de maneira específica para o adenovírus 4 (1CIII e 3DIII) e anticorpo monoclonal apresentando reação cruzada com as espécies de adenovírus 4 e 7 (2HIII). Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo1992-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rimtsp/article/view/28886Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 19-26 Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 19-26 Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 19-26 1678-99460036-4665reponame:Revista do Instituto de Medicina Tropical de São Pauloinstname:Instituto de Medicina Tropical (IMT)instacron:IMTenghttps://www.revistas.usp.br/rimtsp/article/view/28886/30740Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Pauloinfo:eu-repo/semantics/openAccessPaiva, Terezinha Maria deTakimoto, SuekoIshida, María AkíkoSouza, María Candida Oliveira deIshimaru, TuneoNeumann, JorgeKalil, Jorge2012-07-02T01:32:20Zoai:revistas.usp.br:article/28886Revistahttp://www.revistas.usp.br/rimtsp/indexPUBhttps://www.revistas.usp.br/rimtsp/oai||revimtsp@usp.br1678-99460036-4665opendoar:2022-12-13T16:50:39.059081Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)true
dc.title.none.fl_str_mv Comparative study of adenoviruses with monoclonal antibodies
Estudo comparativo de diferentes tipos de adenovirus através de anticorpos monoclonais
title Comparative study of adenoviruses with monoclonal antibodies
spellingShingle Comparative study of adenoviruses with monoclonal antibodies
Paiva, Terezinha Maria de
Adenovirus
Species Specific
Monoclonal antibodies
Adenovirus 4
Antigenic Relationship
title_short Comparative study of adenoviruses with monoclonal antibodies
title_full Comparative study of adenoviruses with monoclonal antibodies
title_fullStr Comparative study of adenoviruses with monoclonal antibodies
title_full_unstemmed Comparative study of adenoviruses with monoclonal antibodies
title_sort Comparative study of adenoviruses with monoclonal antibodies
author Paiva, Terezinha Maria de
author_facet Paiva, Terezinha Maria de
Takimoto, Sueko
Ishida, María Akíko
Souza, María Candida Oliveira de
Ishimaru, Tuneo
Neumann, Jorge
Kalil, Jorge
author_role author
author2 Takimoto, Sueko
Ishida, María Akíko
Souza, María Candida Oliveira de
Ishimaru, Tuneo
Neumann, Jorge
Kalil, Jorge
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Paiva, Terezinha Maria de
Takimoto, Sueko
Ishida, María Akíko
Souza, María Candida Oliveira de
Ishimaru, Tuneo
Neumann, Jorge
Kalil, Jorge
dc.subject.por.fl_str_mv Adenovirus
Species Specific
Monoclonal antibodies
Adenovirus 4
Antigenic Relationship
topic Adenovirus
Species Specific
Monoclonal antibodies
Adenovirus 4
Antigenic Relationship
description The obtainment of monoclonal antibodies for adenovirus species 4(Ad4) is described.The specificities of selected monoclonal antibodies were determined by means of viral neutralization test in cell culture, immunofluorescence and Enzyme-Linked Immunosorbent Assay (ELISA), in the presence of the following species of human adenovirus: 1, 2, 5 (subgenus C), 4 (subgenus E), 7 and 16 (subgenus B) and 9 (subgenus D). Two monoclonal antibodies species specific to adenovirus 4 (1CIII and 3DIII) and one monoclonal antibody that cross reacted with adenovirus species 4 and 7 (2HIII) were obtained.
publishDate 1992
dc.date.none.fl_str_mv 1992-02-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28886
url https://www.revistas.usp.br/rimtsp/article/view/28886
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rimtsp/article/view/28886/30740
dc.rights.driver.fl_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Revista do Instituto de Medicina Tropical de São Paulo
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
publisher.none.fl_str_mv Universidade de São Paulo. Instituto de Medicina Tropical de São Paulo
dc.source.none.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 No. 1 (1992); 19-26
Revista do Instituto de Medicina Tropical de São Paulo; Vol. 34 Núm. 1 (1992); 19-26
Revista do Instituto de Medicina Tropical de São Paulo; v. 34 n. 1 (1992); 19-26
1678-9946
0036-4665
reponame:Revista do Instituto de Medicina Tropical de São Paulo
instname:Instituto de Medicina Tropical (IMT)
instacron:IMT
instname_str Instituto de Medicina Tropical (IMT)
instacron_str IMT
institution IMT
reponame_str Revista do Instituto de Medicina Tropical de São Paulo
collection Revista do Instituto de Medicina Tropical de São Paulo
repository.name.fl_str_mv Revista do Instituto de Medicina Tropical de São Paulo - Instituto de Medicina Tropical (IMT)
repository.mail.fl_str_mv ||revimtsp@usp.br
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