Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women

Detalhes bibliográficos
Autor(a) principal: de-Paris,Fernanda
Data de Publicação: 2011
Outros Autores: Machado,Alice Beatriz Mombach Pinheiro, Gheno,Tailise Conte, Ascoli,Bruna Maria, Oliveira,Kátia Ruschel Pilger de, Barth,Afonso Luis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004
Resumo: Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
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spelling Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant womenStreptococcus agalactiaepolymerase chain reactionculturepregnancyStreptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.Brazilian Society of Infectious Diseases2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004Brazilian Journal of Infectious Diseases v.15 n.4 2011reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702011000400004info:eu-repo/semantics/openAccessde-Paris,FernandaMachado,Alice Beatriz Mombach PinheiroGheno,Tailise ConteAscoli,Bruna MariaOliveira,Kátia Ruschel Pilger deBarth,Afonso Luiseng2011-08-15T00:00:00Zoai:scielo:S1413-86702011000400004Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2011-08-15T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
title Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
spellingShingle Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
de-Paris,Fernanda
Streptococcus agalactiae
polymerase chain reaction
culture
pregnancy
title_short Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
title_full Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
title_fullStr Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
title_full_unstemmed Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
title_sort Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
author de-Paris,Fernanda
author_facet de-Paris,Fernanda
Machado,Alice Beatriz Mombach Pinheiro
Gheno,Tailise Conte
Ascoli,Bruna Maria
Oliveira,Kátia Ruschel Pilger de
Barth,Afonso Luis
author_role author
author2 Machado,Alice Beatriz Mombach Pinheiro
Gheno,Tailise Conte
Ascoli,Bruna Maria
Oliveira,Kátia Ruschel Pilger de
Barth,Afonso Luis
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv de-Paris,Fernanda
Machado,Alice Beatriz Mombach Pinheiro
Gheno,Tailise Conte
Ascoli,Bruna Maria
Oliveira,Kátia Ruschel Pilger de
Barth,Afonso Luis
dc.subject.por.fl_str_mv Streptococcus agalactiae
polymerase chain reaction
culture
pregnancy
topic Streptococcus agalactiae
polymerase chain reaction
culture
pregnancy
description Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1413-86702011000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.15 n.4 2011
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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