Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004 |
Resumo: | Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection. |
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Brazilian Journal of Infectious Diseases |
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spelling |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant womenStreptococcus agalactiaepolymerase chain reactionculturepregnancyStreptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection.Brazilian Society of Infectious Diseases2011-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004Brazilian Journal of Infectious Diseases v.15 n.4 2011reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702011000400004info:eu-repo/semantics/openAccessde-Paris,FernandaMachado,Alice Beatriz Mombach PinheiroGheno,Tailise ConteAscoli,Bruna MariaOliveira,Kátia Ruschel Pilger deBarth,Afonso Luiseng2011-08-15T00:00:00Zoai:scielo:S1413-86702011000400004Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2011-08-15T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
title |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
spellingShingle |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women de-Paris,Fernanda Streptococcus agalactiae polymerase chain reaction culture pregnancy |
title_short |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
title_full |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
title_fullStr |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
title_full_unstemmed |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
title_sort |
Group B Streptococcus detection: comparison of PCR assay and culture as a screening method for pregnant women |
author |
de-Paris,Fernanda |
author_facet |
de-Paris,Fernanda Machado,Alice Beatriz Mombach Pinheiro Gheno,Tailise Conte Ascoli,Bruna Maria Oliveira,Kátia Ruschel Pilger de Barth,Afonso Luis |
author_role |
author |
author2 |
Machado,Alice Beatriz Mombach Pinheiro Gheno,Tailise Conte Ascoli,Bruna Maria Oliveira,Kátia Ruschel Pilger de Barth,Afonso Luis |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
de-Paris,Fernanda Machado,Alice Beatriz Mombach Pinheiro Gheno,Tailise Conte Ascoli,Bruna Maria Oliveira,Kátia Ruschel Pilger de Barth,Afonso Luis |
dc.subject.por.fl_str_mv |
Streptococcus agalactiae polymerase chain reaction culture pregnancy |
topic |
Streptococcus agalactiae polymerase chain reaction culture pregnancy |
description |
Streptococcus agalactiae or group B Streptococcus (GBS) is one of the most important causal agents of serious neonatal infections. Numerous assays have been evaluated for GBS screening in order to validate a fast and efficient method. The aim of this study was to compare the culture technique (established as the gold standard) with the molecular method of polymerase chain reaction (PCR) with specific primers (atr gene). Two hundred and sixty-three samples were analyzed. Vaginal samples were collected, according to the Centers for Disease Control and Prevention (CDC) recommendations, from women over 35 weeks of pregnancy at Hospital de Clínicas de Porto Alegre (HCPA). Two different extraction methods were tested in all samples collected. PCR technique yielded 71 (26.99%) positive results. Sensitivity and specificity for PCR were 100% and 86.88%, respectively. PCR demonstrated a shorter turnaround time than the culture. The molecular methodology proved to be a useful screening for GBS, allowing effective treatment to be initiated in shorter time to prevent newborn infection. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-08-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000400004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702011000400004 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.15 n.4 2011 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
_version_ |
1754209241914146816 |