Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , , , |
Tipo de documento: | Relatório |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Infectious Diseases |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500002 |
Resumo: | Fulminant hepatic failure (FHF) is characterized by massive hepatocellular injury, whose physiopathology is still unclear. Hepatitis B (HBV) is probably the most common viral cause of FHF, while hepatitis A (HAV) virus seem occurs less frequently. However, the host and viral factors that determine the outcome of these infections are poorly understood. In the present study, viral load and genotyping determining regions of HAV and HBV genomes were sequenced. Eight FHF patients and one patient with severe acute hepatitis (SAH) were included. Liver and blood samples were collected during liver transplantation or necropsy procedures. HAV-RNA and HBV-DNA were extracted from serum, biopsy and paraffin liver. Nucleotide sequencing of HAV-RNA was performed from VP1/2A and HBV-DNA from PreS/S region. The amplified samples were quantified by Real-Time PCR. The cases of HAV infection were due to subgenotype IA. The cases of HBV infection were due to genotype A2 and D4. The case of HAV/HBV coinfection was infected by genotype IA and D3. Hepatitis A and B infection were associated with genotypes most prevalent in Brazil. In hepatitis A infection the mean of period evolution was 13 days. In hepatitis B, FHF patients infected by genotype D have a shorter period of evolution than FHF patients infected by genotype A (mean 15 v. 53 days). There was no association with genotype-determining region with the severity of hepatitis, however nucleotide differences and high viral load could be observed among FHF. |
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Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, BrazilFulminant hepatitis failurehepatitis A virus (HAV)hepatitis B virus (HBV) and genotype and viral loadFulminant hepatic failure (FHF) is characterized by massive hepatocellular injury, whose physiopathology is still unclear. Hepatitis B (HBV) is probably the most common viral cause of FHF, while hepatitis A (HAV) virus seem occurs less frequently. However, the host and viral factors that determine the outcome of these infections are poorly understood. In the present study, viral load and genotyping determining regions of HAV and HBV genomes were sequenced. Eight FHF patients and one patient with severe acute hepatitis (SAH) were included. Liver and blood samples were collected during liver transplantation or necropsy procedures. HAV-RNA and HBV-DNA were extracted from serum, biopsy and paraffin liver. Nucleotide sequencing of HAV-RNA was performed from VP1/2A and HBV-DNA from PreS/S region. The amplified samples were quantified by Real-Time PCR. The cases of HAV infection were due to subgenotype IA. The cases of HBV infection were due to genotype A2 and D4. The case of HAV/HBV coinfection was infected by genotype IA and D3. Hepatitis A and B infection were associated with genotypes most prevalent in Brazil. In hepatitis A infection the mean of period evolution was 13 days. In hepatitis B, FHF patients infected by genotype D have a shorter period of evolution than FHF patients infected by genotype A (mean 15 v. 53 days). There was no association with genotype-determining region with the severity of hepatitis, however nucleotide differences and high viral load could be observed among FHF.Brazilian Society of Infectious Diseases2009-10-01info:eu-repo/semantics/reportinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500002Brazilian Journal of Infectious Diseases v.13 n.5 2009reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1590/S1413-86702009000500002info:eu-repo/semantics/openAccessSantos,Damião Carlos Moraes dosMartinho,José Manoel da Silva GomesPacheco-Moreira,Lucio FilgueirasAraújo,Cristina Carvalho Viana deOliveira,Barbara Cristina Euzebio Pereira Dias deLago,Barbara VieiraPinto,Marcelo AlvesPaula,Vanessa Salete deeng2010-04-14T00:00:00Zoai:scielo:S1413-86702009000500002Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2010-04-14T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false |
dc.title.none.fl_str_mv |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
title |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
spellingShingle |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil Santos,Damião Carlos Moraes dos Fulminant hepatitis failure hepatitis A virus (HAV) hepatitis B virus (HBV) and genotype and viral load |
title_short |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
title_full |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
title_fullStr |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
title_full_unstemmed |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
title_sort |
Fulminant hepatitis failure in adults and children from a Public Hospital in Rio de Janeiro, Brazil |
author |
Santos,Damião Carlos Moraes dos |
author_facet |
Santos,Damião Carlos Moraes dos Martinho,José Manoel da Silva Gomes Pacheco-Moreira,Lucio Filgueiras Araújo,Cristina Carvalho Viana de Oliveira,Barbara Cristina Euzebio Pereira Dias de Lago,Barbara Vieira Pinto,Marcelo Alves Paula,Vanessa Salete de |
author_role |
author |
author2 |
Martinho,José Manoel da Silva Gomes Pacheco-Moreira,Lucio Filgueiras Araújo,Cristina Carvalho Viana de Oliveira,Barbara Cristina Euzebio Pereira Dias de Lago,Barbara Vieira Pinto,Marcelo Alves Paula,Vanessa Salete de |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Santos,Damião Carlos Moraes dos Martinho,José Manoel da Silva Gomes Pacheco-Moreira,Lucio Filgueiras Araújo,Cristina Carvalho Viana de Oliveira,Barbara Cristina Euzebio Pereira Dias de Lago,Barbara Vieira Pinto,Marcelo Alves Paula,Vanessa Salete de |
dc.subject.por.fl_str_mv |
Fulminant hepatitis failure hepatitis A virus (HAV) hepatitis B virus (HBV) and genotype and viral load |
topic |
Fulminant hepatitis failure hepatitis A virus (HAV) hepatitis B virus (HBV) and genotype and viral load |
description |
Fulminant hepatic failure (FHF) is characterized by massive hepatocellular injury, whose physiopathology is still unclear. Hepatitis B (HBV) is probably the most common viral cause of FHF, while hepatitis A (HAV) virus seem occurs less frequently. However, the host and viral factors that determine the outcome of these infections are poorly understood. In the present study, viral load and genotyping determining regions of HAV and HBV genomes were sequenced. Eight FHF patients and one patient with severe acute hepatitis (SAH) were included. Liver and blood samples were collected during liver transplantation or necropsy procedures. HAV-RNA and HBV-DNA were extracted from serum, biopsy and paraffin liver. Nucleotide sequencing of HAV-RNA was performed from VP1/2A and HBV-DNA from PreS/S region. The amplified samples were quantified by Real-Time PCR. The cases of HAV infection were due to subgenotype IA. The cases of HBV infection were due to genotype A2 and D4. The case of HAV/HBV coinfection was infected by genotype IA and D3. Hepatitis A and B infection were associated with genotypes most prevalent in Brazil. In hepatitis A infection the mean of period evolution was 13 days. In hepatitis B, FHF patients infected by genotype D have a shorter period of evolution than FHF patients infected by genotype A (mean 15 v. 53 days). There was no association with genotype-determining region with the severity of hepatitis, however nucleotide differences and high viral load could be observed among FHF. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/report |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
report |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500002 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000500002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1413-86702009000500002 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
publisher.none.fl_str_mv |
Brazilian Society of Infectious Diseases |
dc.source.none.fl_str_mv |
Brazilian Journal of Infectious Diseases v.13 n.5 2009 reponame:Brazilian Journal of Infectious Diseases instname:Brazilian Society of Infectious Diseases (BSID) instacron:BSID |
instname_str |
Brazilian Society of Infectious Diseases (BSID) |
instacron_str |
BSID |
institution |
BSID |
reponame_str |
Brazilian Journal of Infectious Diseases |
collection |
Brazilian Journal of Infectious Diseases |
repository.name.fl_str_mv |
Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID) |
repository.mail.fl_str_mv |
bjid@bjid.org.br||lgoldani@ufrgs.br |
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1754209241061654529 |