Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil

Detalhes bibliográficos
Autor(a) principal: Silva Souza,Adaliany Cecília da
Data de Publicação: 2017
Outros Autores: Souza Marasca,Giórgia de, Kretzmann-Filho,Nélson Alexandre, Dall-Bello,Aline, Kliemann,Dimas Alexandre, Tovo,Cristiane Valle, Veiga,Ana Beatriz Gorini da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Infectious Diseases
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500525
Resumo: Abstract Infection by hepatitis B virus (HBV) is a worldwide public health problem. Chronic HBV infection with high viral replication may lead to cirrhosis and/or hepatocellular carcinoma. Mutant HBV strains, such as the HBV A1762T/G1764A double mutant, have been associated with poor prognosis and higher risk of the patient for developing cirrhosis and/or hepatocellular carcinoma. This study analyzed the presence of the HBV A1762T/G1764A double mutant in patients with chronic HBV and its association with clinical parameters such as viral load, aminotransferases, and HBV antigens. A total of 49 patients with chronic hepatitis B were included in the study, and the HBV A1762T/G1764A double mutant strain was detected in four samples (8.16%) by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP). The viral load was not significantly different between patients with or without the double mutant strain (p = 0.43). On the other hand, carriers of the HBV A1762T/G1764A double mutant had higher levels of ALT (p = 0.0028), while AST levels did not differ between groups (p = 0.051). In this study, 75% of the samples with the HBV A1762T/G1764A double mutation were HBeAg negative and anti-HBe positive, reflecting seroconversion even though they still displayed high viral loads. Our study has shown that the HBV A1762T/G1764A double mutant strain circulates in Brazilian patients, and is associated with elevated levels of ALT and HBeAg seroconversion.
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spelling Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern BrazilHepatitis BHBVA1762T/G1764A mutationAbstract Infection by hepatitis B virus (HBV) is a worldwide public health problem. Chronic HBV infection with high viral replication may lead to cirrhosis and/or hepatocellular carcinoma. Mutant HBV strains, such as the HBV A1762T/G1764A double mutant, have been associated with poor prognosis and higher risk of the patient for developing cirrhosis and/or hepatocellular carcinoma. This study analyzed the presence of the HBV A1762T/G1764A double mutant in patients with chronic HBV and its association with clinical parameters such as viral load, aminotransferases, and HBV antigens. A total of 49 patients with chronic hepatitis B were included in the study, and the HBV A1762T/G1764A double mutant strain was detected in four samples (8.16%) by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP). The viral load was not significantly different between patients with or without the double mutant strain (p = 0.43). On the other hand, carriers of the HBV A1762T/G1764A double mutant had higher levels of ALT (p = 0.0028), while AST levels did not differ between groups (p = 0.051). In this study, 75% of the samples with the HBV A1762T/G1764A double mutation were HBeAg negative and anti-HBe positive, reflecting seroconversion even though they still displayed high viral loads. Our study has shown that the HBV A1762T/G1764A double mutant strain circulates in Brazilian patients, and is associated with elevated levels of ALT and HBeAg seroconversion.Brazilian Society of Infectious Diseases2017-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500525Brazilian Journal of Infectious Diseases v.21 n.5 2017reponame:Brazilian Journal of Infectious Diseasesinstname:Brazilian Society of Infectious Diseases (BSID)instacron:BSID10.1016/j.bjid.2017.05.002info:eu-repo/semantics/openAccessSilva Souza,Adaliany Cecília daSouza Marasca,Giórgia deKretzmann-Filho,Nélson AlexandreDall-Bello,AlineKliemann,Dimas AlexandreTovo,Cristiane ValleVeiga,Ana Beatriz Gorini daeng2017-10-23T00:00:00Zoai:scielo:S1413-86702017000500525Revistahttps://www.bjid.org.br/https://old.scielo.br/oai/scielo-oai.phpbjid@bjid.org.br||lgoldani@ufrgs.br1678-43911413-8670opendoar:2017-10-23T00:00Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)false
dc.title.none.fl_str_mv Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
title Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
spellingShingle Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
Silva Souza,Adaliany Cecília da
Hepatitis B
HBV
A1762T/G1764A mutation
title_short Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
title_full Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
title_fullStr Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
title_full_unstemmed Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
title_sort Identification of hepatitis B virus A1762T/G1764A double mutant strain in patients in Southern Brazil
author Silva Souza,Adaliany Cecília da
author_facet Silva Souza,Adaliany Cecília da
Souza Marasca,Giórgia de
Kretzmann-Filho,Nélson Alexandre
Dall-Bello,Aline
Kliemann,Dimas Alexandre
Tovo,Cristiane Valle
Veiga,Ana Beatriz Gorini da
author_role author
author2 Souza Marasca,Giórgia de
Kretzmann-Filho,Nélson Alexandre
Dall-Bello,Aline
Kliemann,Dimas Alexandre
Tovo,Cristiane Valle
Veiga,Ana Beatriz Gorini da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva Souza,Adaliany Cecília da
Souza Marasca,Giórgia de
Kretzmann-Filho,Nélson Alexandre
Dall-Bello,Aline
Kliemann,Dimas Alexandre
Tovo,Cristiane Valle
Veiga,Ana Beatriz Gorini da
dc.subject.por.fl_str_mv Hepatitis B
HBV
A1762T/G1764A mutation
topic Hepatitis B
HBV
A1762T/G1764A mutation
description Abstract Infection by hepatitis B virus (HBV) is a worldwide public health problem. Chronic HBV infection with high viral replication may lead to cirrhosis and/or hepatocellular carcinoma. Mutant HBV strains, such as the HBV A1762T/G1764A double mutant, have been associated with poor prognosis and higher risk of the patient for developing cirrhosis and/or hepatocellular carcinoma. This study analyzed the presence of the HBV A1762T/G1764A double mutant in patients with chronic HBV and its association with clinical parameters such as viral load, aminotransferases, and HBV antigens. A total of 49 patients with chronic hepatitis B were included in the study, and the HBV A1762T/G1764A double mutant strain was detected in four samples (8.16%) by polymerase chain reaction followed by restriction fragment length analysis (PCR-RFLP). The viral load was not significantly different between patients with or without the double mutant strain (p = 0.43). On the other hand, carriers of the HBV A1762T/G1764A double mutant had higher levels of ALT (p = 0.0028), while AST levels did not differ between groups (p = 0.051). In this study, 75% of the samples with the HBV A1762T/G1764A double mutation were HBeAg negative and anti-HBe positive, reflecting seroconversion even though they still displayed high viral loads. Our study has shown that the HBV A1762T/G1764A double mutant strain circulates in Brazilian patients, and is associated with elevated levels of ALT and HBeAg seroconversion.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500525
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000500525
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjid.2017.05.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
publisher.none.fl_str_mv Brazilian Society of Infectious Diseases
dc.source.none.fl_str_mv Brazilian Journal of Infectious Diseases v.21 n.5 2017
reponame:Brazilian Journal of Infectious Diseases
instname:Brazilian Society of Infectious Diseases (BSID)
instacron:BSID
instname_str Brazilian Society of Infectious Diseases (BSID)
instacron_str BSID
institution BSID
reponame_str Brazilian Journal of Infectious Diseases
collection Brazilian Journal of Infectious Diseases
repository.name.fl_str_mv Brazilian Journal of Infectious Diseases - Brazilian Society of Infectious Diseases (BSID)
repository.mail.fl_str_mv bjid@bjid.org.br||lgoldani@ufrgs.br
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