Evaluation of minimum detection limit to hepatitis B virus (HBV) PCR “nested

• Main Author: Coser, Tiago Bottin
• Publication Date: 2008
• Other Authors: Chesky, Marisa, de-Paris, Fernanda, Barth, Afonso Luis, Schmitt, Virginia Minghelli, Pinheiro Machado, Alice Beatriz Mombach
• Format: Article
• Language: por
• Source: Clinical and Biomedical Research
• Download full: https://seer.ufrgs.br/index.php/hcpa/article/view/2945
• topic: HBV; PCR; Hepatite B; Hepatite B

All over the world, the hepatitis B virus (HBV) is considered one of the major problems of public health, despite vaccination. World Health Organization (WHO) estimates that more than 2 billions of persons are infected by HBV. Brazil is classified as an area of intermediary incidence by WHO. However, prevalence studies have detected differences of infection indexes in geographic re-gions: 8% in the Amazonian region, 2,5% in middle-west and Northeast, 2% in Southeast and 1% in South. A sensitive and specific diagnosis is very important to the HBV carrier patients. The aim of this study was to determine the minimum limit of detection of the nested PCR in house technique for HBV. Serial dilutions of one quantified sample of HBV (1000 copies/mL; 750 copies/mL; 500 copies/mL; 250 copies/mL) were submitted to a nested PCR. The target of PCR was viral core and pre-core region. Commercial kit, QiAmp, was employed to purify nucleic acids from the sample. The minimum detection limit found was 500 copies/mL or 10 copies per PCR reaction.